Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus
Bacillus cereus sensu lato strain CCBLR15 is highly efficient in inducing in vitro the mortality of coffee-parasitic nematodes, including Pratylenchus coffeae and Radopholus duriophilus. In the present study, this strain was further characterised to investigate (1) its taxonomic position, (2) the secreted/non-secreted nature of its nematicidal compounds, and (3) its in planta biocontrol capacity towards R. duriophilus. Firstly, analysis of 16S rRNA gene sequences and motility test indicated that CCBLR15 was closely related to B. cereus sensu stricto. Secondly, nematodes separate expositions in vitro to CCBLR15 culture filtrate and cell lysate revealed that nematicidal activity was attributed to compounds released after bacterial cell lysis. Thirdly, CCBLR15 nematode biocontrol capacity was studied using three Coffea arabica cultivars, either seed- or cutting-propagated. Plants were therefore characterised by different developmental stages, referred to as 'early' and 'advanced', with respectively 2-3 and 6-8 leaf pairs. Additionally, plants were grown in unsterilised soil, with a one-month delay between inoculations of bacteria and nematodes to assess the persistence of the nematicidal effects. The presence of R. duriophilus in roots decreased plant growth only in one cultivar whose plants were inoculated at the 'early' stage. Nonetheless, CCBLR15 pre-inoculation significantly reduced the number of nematodes in roots of all cultivars and inhibited the negative effects of nematodes in 'early'-inoculated plants. These results demonstrate the capacity of CCBLR15 to control R. duriophilus and constitute an important step before studying the persistence of its biocontrol effects on a longer period or after plants establishment in the field.
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Subjects: | H01 - Protection des végétaux - Considérations générales, H50 - Troubles divers des plantes, lutte antinématode, endophyte, nématode des plantes, nématocide, Bacillus cereus, protection biologique des plantes, Coffea arabica, http://aims.fao.org/aos/agrovoc/c_5110, http://aims.fao.org/aos/agrovoc/c_32439, http://aims.fao.org/aos/agrovoc/c_5972, http://aims.fao.org/aos/agrovoc/c_5109, http://aims.fao.org/aos/agrovoc/c_26384, http://aims.fao.org/aos/agrovoc/c_35818, http://aims.fao.org/aos/agrovoc/c_1721, |
Online Access: | http://agritrop.cirad.fr/600924/ http://agritrop.cirad.fr/600924/7/600924.pdf |
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dig-cirad-fr-6009242024-01-29T19:05:03Z http://agritrop.cirad.fr/600924/ http://agritrop.cirad.fr/600924/ Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus. Duong Benoit, Marraccini Pierre, Etienne Hervé, Villain Luc, Hoang Thi Giang, Khong Ngan Giang, Lebrun Michel, Duponnois Robin. 2022. Biocontrol Science and Technology, 32 (8) : 971-988.https://doi.org/10.1080/09583157.2022.2070600 <https://doi.org/10.1080/09583157.2022.2070600> Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus Duong, Benoit Marraccini, Pierre Etienne, Hervé Villain, Luc Hoang, Thi Giang Khong, Ngan Giang Lebrun, Michel Duponnois, Robin eng 2022 Biocontrol Science and Technology H01 - Protection des végétaux - Considérations générales H50 - Troubles divers des plantes lutte antinématode endophyte nématode des plantes nématocide Bacillus cereus protection biologique des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_5110 http://aims.fao.org/aos/agrovoc/c_32439 http://aims.fao.org/aos/agrovoc/c_5972 http://aims.fao.org/aos/agrovoc/c_5109 http://aims.fao.org/aos/agrovoc/c_26384 http://aims.fao.org/aos/agrovoc/c_35818 http://aims.fao.org/aos/agrovoc/c_1721 Bacillus cereus sensu lato strain CCBLR15 is highly efficient in inducing in vitro the mortality of coffee-parasitic nematodes, including Pratylenchus coffeae and Radopholus duriophilus. In the present study, this strain was further characterised to investigate (1) its taxonomic position, (2) the secreted/non-secreted nature of its nematicidal compounds, and (3) its in planta biocontrol capacity towards R. duriophilus. Firstly, analysis of 16S rRNA gene sequences and motility test indicated that CCBLR15 was closely related to B. cereus sensu stricto. Secondly, nematodes separate expositions in vitro to CCBLR15 culture filtrate and cell lysate revealed that nematicidal activity was attributed to compounds released after bacterial cell lysis. Thirdly, CCBLR15 nematode biocontrol capacity was studied using three Coffea arabica cultivars, either seed- or cutting-propagated. Plants were therefore characterised by different developmental stages, referred to as 'early' and 'advanced', with respectively 2-3 and 6-8 leaf pairs. Additionally, plants were grown in unsterilised soil, with a one-month delay between inoculations of bacteria and nematodes to assess the persistence of the nematicidal effects. The presence of R. duriophilus in roots decreased plant growth only in one cultivar whose plants were inoculated at the 'early' stage. Nonetheless, CCBLR15 pre-inoculation significantly reduced the number of nematodes in roots of all cultivars and inhibited the negative effects of nematodes in 'early'-inoculated plants. These results demonstrate the capacity of CCBLR15 to control R. duriophilus and constitute an important step before studying the persistence of its biocontrol effects on a longer period or after plants establishment in the field. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/600924/7/600924.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1080/09583157.2022.2070600 10.1080/09583157.2022.2070600 info:eu-repo/semantics/altIdentifier/doi/10.1080/09583157.2022.2070600 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1080/09583157.2022.2070600 info:eu-repo/grantAgreement/EC/H2020/727934//(EU) BREEDing Coffee for AgroForestry Systems/BREEDCAFS |
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H01 - Protection des végétaux - Considérations générales H50 - Troubles divers des plantes lutte antinématode endophyte nématode des plantes nématocide Bacillus cereus protection biologique des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_5110 http://aims.fao.org/aos/agrovoc/c_32439 http://aims.fao.org/aos/agrovoc/c_5972 http://aims.fao.org/aos/agrovoc/c_5109 http://aims.fao.org/aos/agrovoc/c_26384 http://aims.fao.org/aos/agrovoc/c_35818 http://aims.fao.org/aos/agrovoc/c_1721 H01 - Protection des végétaux - Considérations générales H50 - Troubles divers des plantes lutte antinématode endophyte nématode des plantes nématocide Bacillus cereus protection biologique des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_5110 http://aims.fao.org/aos/agrovoc/c_32439 http://aims.fao.org/aos/agrovoc/c_5972 http://aims.fao.org/aos/agrovoc/c_5109 http://aims.fao.org/aos/agrovoc/c_26384 http://aims.fao.org/aos/agrovoc/c_35818 http://aims.fao.org/aos/agrovoc/c_1721 |
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H01 - Protection des végétaux - Considérations générales H50 - Troubles divers des plantes lutte antinématode endophyte nématode des plantes nématocide Bacillus cereus protection biologique des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_5110 http://aims.fao.org/aos/agrovoc/c_32439 http://aims.fao.org/aos/agrovoc/c_5972 http://aims.fao.org/aos/agrovoc/c_5109 http://aims.fao.org/aos/agrovoc/c_26384 http://aims.fao.org/aos/agrovoc/c_35818 http://aims.fao.org/aos/agrovoc/c_1721 H01 - Protection des végétaux - Considérations générales H50 - Troubles divers des plantes lutte antinématode endophyte nématode des plantes nématocide Bacillus cereus protection biologique des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_5110 http://aims.fao.org/aos/agrovoc/c_32439 http://aims.fao.org/aos/agrovoc/c_5972 http://aims.fao.org/aos/agrovoc/c_5109 http://aims.fao.org/aos/agrovoc/c_26384 http://aims.fao.org/aos/agrovoc/c_35818 http://aims.fao.org/aos/agrovoc/c_1721 Duong, Benoit Marraccini, Pierre Etienne, Hervé Villain, Luc Hoang, Thi Giang Khong, Ngan Giang Lebrun, Michel Duponnois, Robin Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus |
description |
Bacillus cereus sensu lato strain CCBLR15 is highly efficient in inducing in vitro the mortality of coffee-parasitic nematodes, including Pratylenchus coffeae and Radopholus duriophilus. In the present study, this strain was further characterised to investigate (1) its taxonomic position, (2) the secreted/non-secreted nature of its nematicidal compounds, and (3) its in planta biocontrol capacity towards R. duriophilus. Firstly, analysis of 16S rRNA gene sequences and motility test indicated that CCBLR15 was closely related to B. cereus sensu stricto. Secondly, nematodes separate expositions in vitro to CCBLR15 culture filtrate and cell lysate revealed that nematicidal activity was attributed to compounds released after bacterial cell lysis. Thirdly, CCBLR15 nematode biocontrol capacity was studied using three Coffea arabica cultivars, either seed- or cutting-propagated. Plants were therefore characterised by different developmental stages, referred to as 'early' and 'advanced', with respectively 2-3 and 6-8 leaf pairs. Additionally, plants were grown in unsterilised soil, with a one-month delay between inoculations of bacteria and nematodes to assess the persistence of the nematicidal effects. The presence of R. duriophilus in roots decreased plant growth only in one cultivar whose plants were inoculated at the 'early' stage. Nonetheless, CCBLR15 pre-inoculation significantly reduced the number of nematodes in roots of all cultivars and inhibited the negative effects of nematodes in 'early'-inoculated plants. These results demonstrate the capacity of CCBLR15 to control R. duriophilus and constitute an important step before studying the persistence of its biocontrol effects on a longer period or after plants establishment in the field. |
format |
article |
topic_facet |
H01 - Protection des végétaux - Considérations générales H50 - Troubles divers des plantes lutte antinématode endophyte nématode des plantes nématocide Bacillus cereus protection biologique des plantes Coffea arabica http://aims.fao.org/aos/agrovoc/c_5110 http://aims.fao.org/aos/agrovoc/c_32439 http://aims.fao.org/aos/agrovoc/c_5972 http://aims.fao.org/aos/agrovoc/c_5109 http://aims.fao.org/aos/agrovoc/c_26384 http://aims.fao.org/aos/agrovoc/c_35818 http://aims.fao.org/aos/agrovoc/c_1721 |
author |
Duong, Benoit Marraccini, Pierre Etienne, Hervé Villain, Luc Hoang, Thi Giang Khong, Ngan Giang Lebrun, Michel Duponnois, Robin |
author_facet |
Duong, Benoit Marraccini, Pierre Etienne, Hervé Villain, Luc Hoang, Thi Giang Khong, Ngan Giang Lebrun, Michel Duponnois, Robin |
author_sort |
Duong, Benoit |
title |
Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus |
title_short |
Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus |
title_full |
Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus |
title_fullStr |
Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus |
title_full_unstemmed |
Potential of the coffee endophytic Bacillus cereus sensu lato strain CCBLR15 to control the plant-parasitic nematode Radopholus duriophilus |
title_sort |
potential of the coffee endophytic bacillus cereus sensu lato strain ccblr15 to control the plant-parasitic nematode radopholus duriophilus |
url |
http://agritrop.cirad.fr/600924/ http://agritrop.cirad.fr/600924/7/600924.pdf |
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