RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines
Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different P. halstedii pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different Pl genes. High-throughput sequencing of cDNA from P. halstedii led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six P. halstedii putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of P. halstedii effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in Pl loci-mediated resistances.
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Subjects: | résistance aux maladies, pathotype, effecteur moléculaire, résistance génétique, Plasmopara halstedii, Helianthus annuus, expression des gènes, pouvoir pathogène, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_24059, http://aims.fao.org/aos/agrovoc/c_27506, http://aims.fao.org/aos/agrovoc/c_35130, http://aims.fao.org/aos/agrovoc/c_34555, http://aims.fao.org/aos/agrovoc/c_3539, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_5629, |
Online Access: | http://agritrop.cirad.fr/600454/ http://agritrop.cirad.fr/600454/1/Gasuel_et_al_2016_Front_Plant_Sci.pdf |
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dig-cirad-fr-6004542024-01-29T05:46:18Z http://agritrop.cirad.fr/600454/ http://agritrop.cirad.fr/600454/ RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines. Gascuel Quentin, Buendia Luis, Pecrix Yann, Blanchet Nicolas, Muños Stéphane, Vear Felicity, Godiard Laurence. 2016. Frontiers in Plant Science, 7:1887, 15 p.https://doi.org/10.3389/fpls.2016.01887 <https://doi.org/10.3389/fpls.2016.01887> RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines Gascuel, Quentin Buendia, Luis Pecrix, Yann Blanchet, Nicolas Muños, Stéphane Vear, Felicity Godiard, Laurence eng 2016 Frontiers in Plant Science résistance aux maladies pathotype effecteur moléculaire résistance génétique Plasmopara halstedii Helianthus annuus expression des gènes pouvoir pathogène http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24059 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_34555 http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5629 Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different P. halstedii pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different Pl genes. High-throughput sequencing of cDNA from P. halstedii led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six P. halstedii putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of P. halstedii effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in Pl loci-mediated resistances. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/600454/1/Gasuel_et_al_2016_Front_Plant_Sci.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3389/fpls.2016.01887 10.3389/fpls.2016.01887 info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2016.01887 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3389/fpls.2016.01887 |
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résistance aux maladies pathotype effecteur moléculaire résistance génétique Plasmopara halstedii Helianthus annuus expression des gènes pouvoir pathogène http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24059 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_34555 http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5629 résistance aux maladies pathotype effecteur moléculaire résistance génétique Plasmopara halstedii Helianthus annuus expression des gènes pouvoir pathogène http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24059 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_34555 http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5629 |
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résistance aux maladies pathotype effecteur moléculaire résistance génétique Plasmopara halstedii Helianthus annuus expression des gènes pouvoir pathogène http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24059 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_34555 http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5629 résistance aux maladies pathotype effecteur moléculaire résistance génétique Plasmopara halstedii Helianthus annuus expression des gènes pouvoir pathogène http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24059 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_34555 http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5629 Gascuel, Quentin Buendia, Luis Pecrix, Yann Blanchet, Nicolas Muños, Stéphane Vear, Felicity Godiard, Laurence RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines |
description |
Plasmopara halstedii is an obligate biotrophic oomycete causing downy mildew disease on sunflower, Helianthus annuus, an economically important oil crop. Severe symptoms of the disease (e.g., plant dwarfism, leaf bleaching, sporulation and production of infertile flower) strongly impair seed yield. Pl resistance genes conferring resistance to specific P. halstedii pathotypes were located on sunflower genetic map but yet not cloned. They are present in cultivated lines to protect them against downy mildew disease. Among the 16 different P. halstedii pathotypes recorded in France, pathotype 710 is frequently found, and therefore continuously controlled in sunflower by different Pl genes. High-throughput sequencing of cDNA from P. halstedii led us to identify potential effectors with the characteristic RXLR or CRN motifs described in other oomycetes. Expression of six P. halstedii putative effectors, five RXLR and one CRN, was analyzed by qRT-PCR in pathogen spores and in the pathogen infecting sunflower leaves and selected for functional analyses. We developed a new method for transient expression in sunflower plant leaves and showed for the first time subcellular localization of P. halstedii effectors fused to a fluorescent protein in sunflower leaf cells. Overexpression of the CRN and of 3 RXLR effectors induced hypersensitive-like cell death reactions in some sunflower near-isogenic lines resistant to pathotype 710 and not in susceptible corresponding lines, suggesting they could be involved in Pl loci-mediated resistances. |
format |
article |
topic_facet |
résistance aux maladies pathotype effecteur moléculaire résistance génétique Plasmopara halstedii Helianthus annuus expression des gènes pouvoir pathogène http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_24059 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_34555 http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5629 |
author |
Gascuel, Quentin Buendia, Luis Pecrix, Yann Blanchet, Nicolas Muños, Stéphane Vear, Felicity Godiard, Laurence |
author_facet |
Gascuel, Quentin Buendia, Luis Pecrix, Yann Blanchet, Nicolas Muños, Stéphane Vear, Felicity Godiard, Laurence |
author_sort |
Gascuel, Quentin |
title |
RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines |
title_short |
RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines |
title_full |
RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines |
title_fullStr |
RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines |
title_full_unstemmed |
RXLR and CRN effectors from the sunflower downy mildew pathogen Plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines |
title_sort |
rxlr and crn effectors from the sunflower downy mildew pathogen plasmopara halstedii induce hypersensitive-like responses in resistant sunflower lines |
url |
http://agritrop.cirad.fr/600454/ http://agritrop.cirad.fr/600454/1/Gasuel_et_al_2016_Front_Plant_Sci.pdf |
work_keys_str_mv |
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1792500317208707072 |