Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus
Aedes albopictus is a major vector of arboviruses. Better understanding of its sex determination is crucial for developing mosquito control tools, especially genetic sexing strains. In Aedes aegypti, Nix is the primary gene responsible for masculinization and Nix-expressing genetic females develop into fertile, albeit flightless, males. In Ae. albopictus, Nix has also been implicated in masculinization but its role remains to be further characterized. In this work, we establish Ae. albopictus transgenic lines ectopically expressing Nix. Several are composed exclusively of genetic females, with transgenic individuals being phenotypic and functional males due to the expression of the Nix transgene. Their reproductive fitness is marginally impaired, while their flight performance is similar to controls. Overall, our results show that Nix is sufficient for full masculinization in Ae. albopictus. Moreover, the transgene construct contains a fluorescence marker allowing efficient automated sex sorting. Consequently, such strains constitute valuable sexing strains for genetic control.
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Subjects: | H10 - Ravageurs des plantes, S50 - Santé humaine, Aedes albopictus, vecteur de maladie, lutte anti-insecte, génomique fonctionnelle, expression des gènes, gène, masculinisation, génétique moléculaire, sex ratio, http://aims.fao.org/aos/agrovoc/c_30483, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_3885, http://aims.fao.org/aos/agrovoc/c_4112bc6e, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_51b187f7, http://aims.fao.org/aos/agrovoc/c_27577, http://aims.fao.org/aos/agrovoc/c_7014, |
Online Access: | http://agritrop.cirad.fr/600403/ http://agritrop.cirad.fr/600403/1/Publi%20Nix%20Celia_Comm%20Biology_2022.pdf |
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dig-cirad-fr-6004032024-01-29T19:02:29Z http://agritrop.cirad.fr/600403/ http://agritrop.cirad.fr/600403/ Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus. Lutrat Célia, Olmo Roenick P., Baldet Thierry, Bouyer Jérémy, Marois Eric. 2022. Communications Biology, 5:210, 10 p.https://doi.org/10.1038/s42003-022-03165-7 <https://doi.org/10.1038/s42003-022-03165-7> Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus Lutrat, Célia Olmo, Roenick P. Baldet, Thierry Bouyer, Jérémy Marois, Eric eng 2022 Communications Biology H10 - Ravageurs des plantes S50 - Santé humaine Aedes albopictus vecteur de maladie lutte anti-insecte génomique fonctionnelle expression des gènes gène masculinisation génétique moléculaire sex ratio http://aims.fao.org/aos/agrovoc/c_30483 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_4112bc6e http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_51b187f7 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_7014 Aedes albopictus is a major vector of arboviruses. Better understanding of its sex determination is crucial for developing mosquito control tools, especially genetic sexing strains. In Aedes aegypti, Nix is the primary gene responsible for masculinization and Nix-expressing genetic females develop into fertile, albeit flightless, males. In Ae. albopictus, Nix has also been implicated in masculinization but its role remains to be further characterized. In this work, we establish Ae. albopictus transgenic lines ectopically expressing Nix. Several are composed exclusively of genetic females, with transgenic individuals being phenotypic and functional males due to the expression of the Nix transgene. Their reproductive fitness is marginally impaired, while their flight performance is similar to controls. Overall, our results show that Nix is sufficient for full masculinization in Ae. albopictus. Moreover, the transgene construct contains a fluorescence marker allowing efficient automated sex sorting. Consequently, such strains constitute valuable sexing strains for genetic control. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/600403/1/Publi%20Nix%20Celia_Comm%20Biology_2022.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1038/s42003-022-03165-7 10.1038/s42003-022-03165-7 info:eu-repo/semantics/altIdentifier/doi/10.1038/s42003-022-03165-7 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/s42003-022-03165-7 info:eu-repo/semantics/dataset/purl/https://www.addgene.org/search/catalog/plasmids/?q=%23173505 info:eu-repo/grantAgreement/EC/H2020/682387//(EU) Revolutionizing Insect Control/REVOLINC |
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H10 - Ravageurs des plantes S50 - Santé humaine Aedes albopictus vecteur de maladie lutte anti-insecte génomique fonctionnelle expression des gènes gène masculinisation génétique moléculaire sex ratio http://aims.fao.org/aos/agrovoc/c_30483 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_4112bc6e http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_51b187f7 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_7014 H10 - Ravageurs des plantes S50 - Santé humaine Aedes albopictus vecteur de maladie lutte anti-insecte génomique fonctionnelle expression des gènes gène masculinisation génétique moléculaire sex ratio http://aims.fao.org/aos/agrovoc/c_30483 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_4112bc6e http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_51b187f7 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_7014 |
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H10 - Ravageurs des plantes S50 - Santé humaine Aedes albopictus vecteur de maladie lutte anti-insecte génomique fonctionnelle expression des gènes gène masculinisation génétique moléculaire sex ratio http://aims.fao.org/aos/agrovoc/c_30483 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_4112bc6e http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_51b187f7 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_7014 H10 - Ravageurs des plantes S50 - Santé humaine Aedes albopictus vecteur de maladie lutte anti-insecte génomique fonctionnelle expression des gènes gène masculinisation génétique moléculaire sex ratio http://aims.fao.org/aos/agrovoc/c_30483 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_4112bc6e http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_51b187f7 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_7014 Lutrat, Célia Olmo, Roenick P. Baldet, Thierry Bouyer, Jérémy Marois, Eric Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus |
description |
Aedes albopictus is a major vector of arboviruses. Better understanding of its sex determination is crucial for developing mosquito control tools, especially genetic sexing strains. In Aedes aegypti, Nix is the primary gene responsible for masculinization and Nix-expressing genetic females develop into fertile, albeit flightless, males. In Ae. albopictus, Nix has also been implicated in masculinization but its role remains to be further characterized. In this work, we establish Ae. albopictus transgenic lines ectopically expressing Nix. Several are composed exclusively of genetic females, with transgenic individuals being phenotypic and functional males due to the expression of the Nix transgene. Their reproductive fitness is marginally impaired, while their flight performance is similar to controls. Overall, our results show that Nix is sufficient for full masculinization in Ae. albopictus. Moreover, the transgene construct contains a fluorescence marker allowing efficient automated sex sorting. Consequently, such strains constitute valuable sexing strains for genetic control. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes S50 - Santé humaine Aedes albopictus vecteur de maladie lutte anti-insecte génomique fonctionnelle expression des gènes gène masculinisation génétique moléculaire sex ratio http://aims.fao.org/aos/agrovoc/c_30483 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_3885 http://aims.fao.org/aos/agrovoc/c_4112bc6e http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_51b187f7 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_7014 |
author |
Lutrat, Célia Olmo, Roenick P. Baldet, Thierry Bouyer, Jérémy Marois, Eric |
author_facet |
Lutrat, Célia Olmo, Roenick P. Baldet, Thierry Bouyer, Jérémy Marois, Eric |
author_sort |
Lutrat, Célia |
title |
Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus |
title_short |
Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus |
title_full |
Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus |
title_fullStr |
Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus |
title_full_unstemmed |
Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus |
title_sort |
transgenic expression of nix converts genetic females into males and allows automated sex sorting in aedes albopictus |
url |
http://agritrop.cirad.fr/600403/ http://agritrop.cirad.fr/600403/1/Publi%20Nix%20Celia_Comm%20Biology_2022.pdf |
work_keys_str_mv |
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