Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses
Viral metagenomic studies have enabled the discovery of many unknown viruses and revealed that viral communities are much more diverse and ubiquitous than previously thought. Some viruses have multiple genome components that are encapsidated either in separate virions (multipartite viruses) or in the same virion (segmented viruses). In this study, we identify what is possibly a novel bipartite plant-associated circular single-stranded DNA virus in a wild prickly pear cactus, Opuntia discolor, that is endemic to the Chaco ecoregion in South America. Two ~1.8 kb virus-like circular DNA components were recovered, one encoding a replication-associated protein (Rep) and the other a capsid protein (CP). Both of the inferred protein sequences of the Rep and CP are homologous to those encoded by members of the family Geminiviridae. These two putatively cognate components each have a nonanucleotide sequence within a likely hairpin structure that is homologous to the origins of rolling-circle replication (RCR), found in diverse circular single-stranded DNA viruses. In addition, the two components share similar putative replication-associated iterative sequences (iterons), which in circular single-stranded DNA viruses are important for Rep binding during the initiation of RCR. Such molecular features provide support for the possible bipartite nature of this virus, which we named utkilio virus (common name of the Opuntia discolor in South America) components A and B. In the infectivity assays conducted in Nicotiana benthamiana plants, only the A component of utkilio virus, which encodes the Rep protein, was found to move and replicate systemically in N. benthamiana. This was not true for component B, for which we did not detect replication, which may have been due to this being a defective molecule or because of the model plants (N. benthamiana) used for the infection assays. Future experiments need to be conducted with other plants, including O. discolor, to understand more about the biology of these viral components.
Main Authors: | , , , , , , , , , , , |
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Language: | eng |
Subjects: | Geminiviridae, virus des végétaux, begomovirus, Cocos nucifera, virus ADNsb, virologie, identification, Opuntia, phylogénie, adn, http://aims.fao.org/aos/agrovoc/c_32859, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_61d49fca, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_d469d8f8, http://aims.fao.org/aos/agrovoc/c_8259, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_5373, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_1486, http://aims.fao.org/aos/agrovoc/c_603, http://aims.fao.org/aos/agrovoc/c_331403, http://aims.fao.org/aos/agrovoc/c_1070, |
Online Access: | http://agritrop.cirad.fr/600123/ http://agritrop.cirad.fr/600123/1/Fontenele_Utkilio_2021.pdf |
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Geminiviridae virus des végétaux begomovirus Cocos nucifera virus ADNsb virologie identification Opuntia phylogénie adn http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_d469d8f8 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_5373 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_1486 http://aims.fao.org/aos/agrovoc/c_603 http://aims.fao.org/aos/agrovoc/c_331403 http://aims.fao.org/aos/agrovoc/c_1070 Geminiviridae virus des végétaux begomovirus Cocos nucifera virus ADNsb virologie identification Opuntia phylogénie adn http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_d469d8f8 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_5373 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_1486 http://aims.fao.org/aos/agrovoc/c_603 http://aims.fao.org/aos/agrovoc/c_331403 http://aims.fao.org/aos/agrovoc/c_1070 |
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Geminiviridae virus des végétaux begomovirus Cocos nucifera virus ADNsb virologie identification Opuntia phylogénie adn http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_d469d8f8 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_5373 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_1486 http://aims.fao.org/aos/agrovoc/c_603 http://aims.fao.org/aos/agrovoc/c_331403 http://aims.fao.org/aos/agrovoc/c_1070 Geminiviridae virus des végétaux begomovirus Cocos nucifera virus ADNsb virologie identification Opuntia phylogénie adn http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_d469d8f8 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_5373 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_1486 http://aims.fao.org/aos/agrovoc/c_603 http://aims.fao.org/aos/agrovoc/c_331403 http://aims.fao.org/aos/agrovoc/c_1070 Fontenele, Rafaela S Köhler, Matias Majure, Lucas C. Avalos‐Callero, Jesús A. Argüello‐Astorga, Gerardo R. Font, Fabián Vidal, Andreza H. Roumagnac, Philippe Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses |
description |
Viral metagenomic studies have enabled the discovery of many unknown viruses and revealed that viral communities are much more diverse and ubiquitous than previously thought. Some viruses have multiple genome components that are encapsidated either in separate virions (multipartite viruses) or in the same virion (segmented viruses). In this study, we identify what is possibly a novel bipartite plant-associated circular single-stranded DNA virus in a wild prickly pear cactus, Opuntia discolor, that is endemic to the Chaco ecoregion in South America. Two ~1.8 kb virus-like circular DNA components were recovered, one encoding a replication-associated protein (Rep) and the other a capsid protein (CP). Both of the inferred protein sequences of the Rep and CP are homologous to those encoded by members of the family Geminiviridae. These two putatively cognate components each have a nonanucleotide sequence within a likely hairpin structure that is homologous to the origins of rolling-circle replication (RCR), found in diverse circular single-stranded DNA viruses. In addition, the two components share similar putative replication-associated iterative sequences (iterons), which in circular single-stranded DNA viruses are important for Rep binding during the initiation of RCR. Such molecular features provide support for the possible bipartite nature of this virus, which we named utkilio virus (common name of the Opuntia discolor in South America) components A and B. In the infectivity assays conducted in Nicotiana benthamiana plants, only the A component of utkilio virus, which encodes the Rep protein, was found to move and replicate systemically in N. benthamiana. This was not true for component B, for which we did not detect replication, which may have been due to this being a defective molecule or because of the model plants (N. benthamiana) used for the infection assays. Future experiments need to be conducted with other plants, including O. discolor, to understand more about the biology of these viral components. |
format |
article |
topic_facet |
Geminiviridae virus des végétaux begomovirus Cocos nucifera virus ADNsb virologie identification Opuntia phylogénie adn http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_d469d8f8 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_5373 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_1486 http://aims.fao.org/aos/agrovoc/c_603 http://aims.fao.org/aos/agrovoc/c_331403 http://aims.fao.org/aos/agrovoc/c_1070 |
author |
Fontenele, Rafaela S Köhler, Matias Majure, Lucas C. Avalos‐Callero, Jesús A. Argüello‐Astorga, Gerardo R. Font, Fabián Vidal, Andreza H. Roumagnac, Philippe Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind |
author_facet |
Fontenele, Rafaela S Köhler, Matias Majure, Lucas C. Avalos‐Callero, Jesús A. Argüello‐Astorga, Gerardo R. Font, Fabián Vidal, Andreza H. Roumagnac, Philippe Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind |
author_sort |
Fontenele, Rafaela S |
title |
Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses |
title_short |
Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses |
title_full |
Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses |
title_fullStr |
Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses |
title_full_unstemmed |
Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses |
title_sort |
novel circular dna virus identified in opuntia discolor (cactaceae) that codes for proteins with similarity to those of geminiviruses |
url |
http://agritrop.cirad.fr/600123/ http://agritrop.cirad.fr/600123/1/Fontenele_Utkilio_2021.pdf |
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1792500295096336384 |
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dig-cirad-fr-6001232024-01-29T05:45:27Z http://agritrop.cirad.fr/600123/ http://agritrop.cirad.fr/600123/ Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses. Fontenele Rafaela S, Köhler Matias, Majure Lucas C., Avalos‐Callero Jesús A., Argüello‐Astorga Gerardo R., Font Fabián, Vidal Andreza H., Roumagnac Philippe, Kraberger Simona, Martin Darren Patrick, Lefeuvre Pierre, Varsani Arvind. 2021. Journal of General Virology, 102 (11), 7 p.https://doi.org/10.1099/jgv.0.001671 <https://doi.org/10.1099/jgv.0.001671> Novel circular DNA virus identified in Opuntia discolor (Cactaceae) that codes for proteins with similarity to those of geminiviruses Fontenele, Rafaela S Köhler, Matias Majure, Lucas C. Avalos‐Callero, Jesús A. Argüello‐Astorga, Gerardo R. Font, Fabián Vidal, Andreza H. Roumagnac, Philippe Kraberger, Simona Martin, Darren Patrick Lefeuvre, Pierre Varsani, Arvind eng 2021 Journal of General Virology Geminiviridae virus des végétaux begomovirus Cocos nucifera virus ADNsb virologie identification Opuntia phylogénie adn http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_61d49fca http://aims.fao.org/aos/agrovoc/c_1716 http://aims.fao.org/aos/agrovoc/c_d469d8f8 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_5373 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_2347 Chaco Argentine Bolivie (État plurinational de) Brésil http://aims.fao.org/aos/agrovoc/c_1486 http://aims.fao.org/aos/agrovoc/c_603 http://aims.fao.org/aos/agrovoc/c_331403 http://aims.fao.org/aos/agrovoc/c_1070 Viral metagenomic studies have enabled the discovery of many unknown viruses and revealed that viral communities are much more diverse and ubiquitous than previously thought. Some viruses have multiple genome components that are encapsidated either in separate virions (multipartite viruses) or in the same virion (segmented viruses). In this study, we identify what is possibly a novel bipartite plant-associated circular single-stranded DNA virus in a wild prickly pear cactus, Opuntia discolor, that is endemic to the Chaco ecoregion in South America. Two ~1.8 kb virus-like circular DNA components were recovered, one encoding a replication-associated protein (Rep) and the other a capsid protein (CP). Both of the inferred protein sequences of the Rep and CP are homologous to those encoded by members of the family Geminiviridae. These two putatively cognate components each have a nonanucleotide sequence within a likely hairpin structure that is homologous to the origins of rolling-circle replication (RCR), found in diverse circular single-stranded DNA viruses. In addition, the two components share similar putative replication-associated iterative sequences (iterons), which in circular single-stranded DNA viruses are important for Rep binding during the initiation of RCR. Such molecular features provide support for the possible bipartite nature of this virus, which we named utkilio virus (common name of the Opuntia discolor in South America) components A and B. In the infectivity assays conducted in Nicotiana benthamiana plants, only the A component of utkilio virus, which encodes the Rep protein, was found to move and replicate systemically in N. benthamiana. This was not true for component B, for which we did not detect replication, which may have been due to this being a defective molecule or because of the model plants (N. benthamiana) used for the infection assays. Future experiments need to be conducted with other plants, including O. discolor, to understand more about the biology of these viral components. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/600123/1/Fontenele_Utkilio_2021.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1099/jgv.0.001671 10.1099/jgv.0.001671 info:eu-repo/semantics/altIdentifier/doi/10.1099/jgv.0.001671 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1099/jgv.0.001671 |