A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen
Fusarium oxysporum f. sp. cubense (Foc) is a soil-borne fungus that causes Fusarium wilt, a destructive plant disease that has resulted in devastating economic losses to banana production worldwide. The fungus has a complex evolutionary history and taxonomic repute and consists of three pathogenic races and at least 24 vegetative compatibility groups (VCGs). Surveys conducted in Asia, Africa, the Sultanate of Oman and Mauritius encountered isolates of F. oxysporum pathogenic to banana that were not compatible to any of the known Foc VCGs. Genetic relatedness between the undescribed and known Foc VCGs were determined using a multi-gene phylogeny and diversity array technology (DArT) sequencing. The presence of putative effector genes, the secreted in xylem (SIX) genes, were also determined. Fourteen novel Foc VCGs and 17 single-member VCGs were identified. The multi-gene tree was congruent with the DArT-seq phylogeny and divided the novel VCGs into three clades. Clustering analysis of the DArT-seq data supported the separation of Foc isolates into eight distinct clusters, with the suite of SIX genes mostly conserved within these clusters. Results from this study indicates that Foc is more diverse than hitherto assumed.
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Language: | eng |
Subjects: | H20 - Maladies des plantes, Fusarium oxysporum, flétrissement fusarien, Musa, phylogénie, séquençage à haut débit, technique génétique, http://aims.fao.org/aos/agrovoc/c_16244, http://aims.fao.org/aos/agrovoc/c_a776deb2, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_0a9daef1, http://aims.fao.org/aos/agrovoc/c_331008, |
Online Access: | http://agritrop.cirad.fr/600110/ http://agritrop.cirad.fr/600110/1/microorganisms-10-00269.pdf |
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dig-cirad-fr-6001102024-01-29T19:05:29Z http://agritrop.cirad.fr/600110/ http://agritrop.cirad.fr/600110/ A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen. Mostert Diane, Wicker Emmanuel, de Jager Mignon M., Al Kaabi Saif M., O'Neill Wayne, Perry Suzy, Li Chunyu, Ganyun Yi, Pegg Kenneth G., Mostert Lizel, Viljoen Altus. 2022. Microorganisms, 10 (2):269, 18 p.https://doi.org/10.3390/microorganisms10020269 <https://doi.org/10.3390/microorganisms10020269> A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen Mostert, Diane Wicker, Emmanuel de Jager, Mignon M. Al Kaabi, Saif M. O'Neill, Wayne Perry, Suzy Li, Chunyu Ganyun, Yi Pegg, Kenneth G. Mostert, Lizel Viljoen, Altus eng 2022 Microorganisms H20 - Maladies des plantes Fusarium oxysporum flétrissement fusarien Musa phylogénie séquençage à haut débit technique génétique http://aims.fao.org/aos/agrovoc/c_16244 http://aims.fao.org/aos/agrovoc/c_a776deb2 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_0a9daef1 http://aims.fao.org/aos/agrovoc/c_331008 Fusarium oxysporum f. sp. cubense (Foc) is a soil-borne fungus that causes Fusarium wilt, a destructive plant disease that has resulted in devastating economic losses to banana production worldwide. The fungus has a complex evolutionary history and taxonomic repute and consists of three pathogenic races and at least 24 vegetative compatibility groups (VCGs). Surveys conducted in Asia, Africa, the Sultanate of Oman and Mauritius encountered isolates of F. oxysporum pathogenic to banana that were not compatible to any of the known Foc VCGs. Genetic relatedness between the undescribed and known Foc VCGs were determined using a multi-gene phylogeny and diversity array technology (DArT) sequencing. The presence of putative effector genes, the secreted in xylem (SIX) genes, were also determined. Fourteen novel Foc VCGs and 17 single-member VCGs were identified. The multi-gene tree was congruent with the DArT-seq phylogeny and divided the novel VCGs into three clades. Clustering analysis of the DArT-seq data supported the separation of Foc isolates into eight distinct clusters, with the suite of SIX genes mostly conserved within these clusters. Results from this study indicates that Foc is more diverse than hitherto assumed. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/600110/1/microorganisms-10-00269.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3390/microorganisms10020269 10.3390/microorganisms10020269 info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10020269 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3390/microorganisms10020269 |
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H20 - Maladies des plantes Fusarium oxysporum flétrissement fusarien Musa phylogénie séquençage à haut débit technique génétique http://aims.fao.org/aos/agrovoc/c_16244 http://aims.fao.org/aos/agrovoc/c_a776deb2 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_0a9daef1 http://aims.fao.org/aos/agrovoc/c_331008 H20 - Maladies des plantes Fusarium oxysporum flétrissement fusarien Musa phylogénie séquençage à haut débit technique génétique http://aims.fao.org/aos/agrovoc/c_16244 http://aims.fao.org/aos/agrovoc/c_a776deb2 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_0a9daef1 http://aims.fao.org/aos/agrovoc/c_331008 |
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H20 - Maladies des plantes Fusarium oxysporum flétrissement fusarien Musa phylogénie séquençage à haut débit technique génétique http://aims.fao.org/aos/agrovoc/c_16244 http://aims.fao.org/aos/agrovoc/c_a776deb2 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_0a9daef1 http://aims.fao.org/aos/agrovoc/c_331008 H20 - Maladies des plantes Fusarium oxysporum flétrissement fusarien Musa phylogénie séquençage à haut débit technique génétique http://aims.fao.org/aos/agrovoc/c_16244 http://aims.fao.org/aos/agrovoc/c_a776deb2 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_0a9daef1 http://aims.fao.org/aos/agrovoc/c_331008 Mostert, Diane Wicker, Emmanuel de Jager, Mignon M. Al Kaabi, Saif M. O'Neill, Wayne Perry, Suzy Li, Chunyu Ganyun, Yi Pegg, Kenneth G. Mostert, Lizel Viljoen, Altus A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
description |
Fusarium oxysporum f. sp. cubense (Foc) is a soil-borne fungus that causes Fusarium wilt, a destructive plant disease that has resulted in devastating economic losses to banana production worldwide. The fungus has a complex evolutionary history and taxonomic repute and consists of three pathogenic races and at least 24 vegetative compatibility groups (VCGs). Surveys conducted in Asia, Africa, the Sultanate of Oman and Mauritius encountered isolates of F. oxysporum pathogenic to banana that were not compatible to any of the known Foc VCGs. Genetic relatedness between the undescribed and known Foc VCGs were determined using a multi-gene phylogeny and diversity array technology (DArT) sequencing. The presence of putative effector genes, the secreted in xylem (SIX) genes, were also determined. Fourteen novel Foc VCGs and 17 single-member VCGs were identified. The multi-gene tree was congruent with the DArT-seq phylogeny and divided the novel VCGs into three clades. Clustering analysis of the DArT-seq data supported the separation of Foc isolates into eight distinct clusters, with the suite of SIX genes mostly conserved within these clusters. Results from this study indicates that Foc is more diverse than hitherto assumed. |
format |
article |
topic_facet |
H20 - Maladies des plantes Fusarium oxysporum flétrissement fusarien Musa phylogénie séquençage à haut débit technique génétique http://aims.fao.org/aos/agrovoc/c_16244 http://aims.fao.org/aos/agrovoc/c_a776deb2 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_0a9daef1 http://aims.fao.org/aos/agrovoc/c_331008 |
author |
Mostert, Diane Wicker, Emmanuel de Jager, Mignon M. Al Kaabi, Saif M. O'Neill, Wayne Perry, Suzy Li, Chunyu Ganyun, Yi Pegg, Kenneth G. Mostert, Lizel Viljoen, Altus |
author_facet |
Mostert, Diane Wicker, Emmanuel de Jager, Mignon M. Al Kaabi, Saif M. O'Neill, Wayne Perry, Suzy Li, Chunyu Ganyun, Yi Pegg, Kenneth G. Mostert, Lizel Viljoen, Altus |
author_sort |
Mostert, Diane |
title |
A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
title_short |
A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
title_full |
A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
title_fullStr |
A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
title_full_unstemmed |
A polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
title_sort |
polyphasic approach reveals novel genotypes and updates the genetic structure of the banana fusarium wilt pathogen |
url |
http://agritrop.cirad.fr/600110/ http://agritrop.cirad.fr/600110/1/microorganisms-10-00269.pdf |
work_keys_str_mv |
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