Genetic characterization of African swine fever virus in Cameroon, 2010–2018
African swine fever (ASF) is a highly lethal haemorrhagic disease in domestic and wild swine that has acquired great importance in sub-Saharan Africa since 1997. ASF was first reported in Cameroon in 1982 and was detected only in Southern Cameroon (South, West, East, Northwest, Southwest, Littoral, and Centre regions) until February 2010 when suspected ASF outbreaks were reported in the North and Far North regions. We investigated those outbreaks by analysing samples that were collected from sick pigs between 2010 and 2018. We confirmed 428 positive samples by ELISA and real-time PCR and molecularly characterized 48 representative isolates. All the identified virus isolates were classified as ASFV genotype I based on the partial B646L gene (C-terminal end of VP72 gene) and the full E183L gene encoding p54 protein analysis. Furthermore, analysis of the central variable region (CVR) within the B602L gene demonstrated that there were 3 different variants of ASFV genotype I, with 19, 20, and 21 tetrameric tandem repeat sequences (TRSs), that were involved in the 2010–2018 outbreaks in Cameroon. Among them, only variant A (19 TRSs) was identical to the Cam/82 isolate found in the country during the first outbreaks in 1981–1982. This study demonstrated that the three variants of ASFV isolates involved in these outbreaks were similar to those of neighbouring countries, suggesting a movement of ASFV strains across borders. Designing common control measures in affected regions and providing a compensation programme for farmers will help reduce the incidence and spread of this disease.
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Subjects: | L73 - Maladies des animaux, épidémiologie, virus peste porcine africaine, peste porcine africaine, Enquête pathologique, génétique, maladie des animaux, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_8649, http://aims.fao.org/aos/agrovoc/c_26754, http://aims.fao.org/aos/agrovoc/c_28665, http://aims.fao.org/aos/agrovoc/c_3222, http://aims.fao.org/aos/agrovoc/c_426, http://aims.fao.org/aos/agrovoc/c_1229, |
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dig-cirad-fr-5998962024-01-29T03:56:06Z http://agritrop.cirad.fr/599896/ http://agritrop.cirad.fr/599896/ Genetic characterization of African swine fever virus in Cameroon, 2010–2018. Wade Abel, Achenbach Jenna Elizabeth, Gallardo Carmina, Settypalli Tirumala Bharani Kumar, Souley Abdoulkadiri, Djonwe Gaston, Loitsch Angelika, Dauphin Gwenaelle, Essia Ngang Jean Justin, Boyomo Onana, Cattoli Giovanni, Diallo Adama, Lamien Charles Euloge. 2019. Journal of Microbiology, 57 (4) : 316-324.https://doi.org/10.1007/s12275-019-8457-4 <https://doi.org/10.1007/s12275-019-8457-4> Genetic characterization of African swine fever virus in Cameroon, 2010–2018 Wade, Abel Achenbach, Jenna Elizabeth Gallardo, Carmina Settypalli, Tirumala Bharani Kumar Souley, Abdoulkadiri Djonwe, Gaston Loitsch, Angelika Dauphin, Gwenaelle Essia Ngang, Jean Justin Boyomo, Onana Cattoli, Giovanni Diallo, Adama Lamien, Charles Euloge eng 2019 Journal of Microbiology L73 - Maladies des animaux épidémiologie virus peste porcine africaine peste porcine africaine Enquête pathologique génétique maladie des animaux http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_26754 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_426 Cameroun http://aims.fao.org/aos/agrovoc/c_1229 African swine fever (ASF) is a highly lethal haemorrhagic disease in domestic and wild swine that has acquired great importance in sub-Saharan Africa since 1997. ASF was first reported in Cameroon in 1982 and was detected only in Southern Cameroon (South, West, East, Northwest, Southwest, Littoral, and Centre regions) until February 2010 when suspected ASF outbreaks were reported in the North and Far North regions. We investigated those outbreaks by analysing samples that were collected from sick pigs between 2010 and 2018. We confirmed 428 positive samples by ELISA and real-time PCR and molecularly characterized 48 representative isolates. All the identified virus isolates were classified as ASFV genotype I based on the partial B646L gene (C-terminal end of VP72 gene) and the full E183L gene encoding p54 protein analysis. Furthermore, analysis of the central variable region (CVR) within the B602L gene demonstrated that there were 3 different variants of ASFV genotype I, with 19, 20, and 21 tetrameric tandem repeat sequences (TRSs), that were involved in the 2010–2018 outbreaks in Cameroon. Among them, only variant A (19 TRSs) was identical to the Cam/82 isolate found in the country during the first outbreaks in 1981–1982. This study demonstrated that the three variants of ASFV isolates involved in these outbreaks were similar to those of neighbouring countries, suggesting a movement of ASFV strains across borders. Designing common control measures in affected regions and providing a compensation programme for farmers will help reduce the incidence and spread of this disease. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/599896/1/599896.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s12275-019-8457-4 10.1007/s12275-019-8457-4 info:eu-repo/semantics/altIdentifier/doi/10.1007/s12275-019-8457-4 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s12275-019-8457-4 |
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L73 - Maladies des animaux épidémiologie virus peste porcine africaine peste porcine africaine Enquête pathologique génétique maladie des animaux http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_26754 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_1229 L73 - Maladies des animaux épidémiologie virus peste porcine africaine peste porcine africaine Enquête pathologique génétique maladie des animaux http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_26754 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_1229 |
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L73 - Maladies des animaux épidémiologie virus peste porcine africaine peste porcine africaine Enquête pathologique génétique maladie des animaux http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_26754 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_1229 L73 - Maladies des animaux épidémiologie virus peste porcine africaine peste porcine africaine Enquête pathologique génétique maladie des animaux http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_26754 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_1229 Wade, Abel Achenbach, Jenna Elizabeth Gallardo, Carmina Settypalli, Tirumala Bharani Kumar Souley, Abdoulkadiri Djonwe, Gaston Loitsch, Angelika Dauphin, Gwenaelle Essia Ngang, Jean Justin Boyomo, Onana Cattoli, Giovanni Diallo, Adama Lamien, Charles Euloge Genetic characterization of African swine fever virus in Cameroon, 2010–2018 |
description |
African swine fever (ASF) is a highly lethal haemorrhagic disease in domestic and wild swine that has acquired great importance in sub-Saharan Africa since 1997. ASF was first reported in Cameroon in 1982 and was detected only in Southern Cameroon (South, West, East, Northwest, Southwest, Littoral, and Centre regions) until February 2010 when suspected ASF outbreaks were reported in the North and Far North regions. We investigated those outbreaks by analysing samples that were collected from sick pigs between 2010 and 2018. We confirmed 428 positive samples by ELISA and real-time PCR and molecularly characterized 48 representative isolates. All the identified virus isolates were classified as ASFV genotype I based on the partial B646L gene (C-terminal end of VP72 gene) and the full E183L gene encoding p54 protein analysis. Furthermore, analysis of the central variable region (CVR) within the B602L gene demonstrated that there were 3 different variants of ASFV genotype I, with 19, 20, and 21 tetrameric tandem repeat sequences (TRSs), that were involved in the 2010–2018 outbreaks in Cameroon. Among them, only variant A (19 TRSs) was identical to the Cam/82 isolate found in the country during the first outbreaks in 1981–1982. This study demonstrated that the three variants of ASFV isolates involved in these outbreaks were similar to those of neighbouring countries, suggesting a movement of ASFV strains across borders. Designing common control measures in affected regions and providing a compensation programme for farmers will help reduce the incidence and spread of this disease. |
format |
article |
topic_facet |
L73 - Maladies des animaux épidémiologie virus peste porcine africaine peste porcine africaine Enquête pathologique génétique maladie des animaux http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_26754 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_1229 |
author |
Wade, Abel Achenbach, Jenna Elizabeth Gallardo, Carmina Settypalli, Tirumala Bharani Kumar Souley, Abdoulkadiri Djonwe, Gaston Loitsch, Angelika Dauphin, Gwenaelle Essia Ngang, Jean Justin Boyomo, Onana Cattoli, Giovanni Diallo, Adama Lamien, Charles Euloge |
author_facet |
Wade, Abel Achenbach, Jenna Elizabeth Gallardo, Carmina Settypalli, Tirumala Bharani Kumar Souley, Abdoulkadiri Djonwe, Gaston Loitsch, Angelika Dauphin, Gwenaelle Essia Ngang, Jean Justin Boyomo, Onana Cattoli, Giovanni Diallo, Adama Lamien, Charles Euloge |
author_sort |
Wade, Abel |
title |
Genetic characterization of African swine fever virus in Cameroon, 2010–2018 |
title_short |
Genetic characterization of African swine fever virus in Cameroon, 2010–2018 |
title_full |
Genetic characterization of African swine fever virus in Cameroon, 2010–2018 |
title_fullStr |
Genetic characterization of African swine fever virus in Cameroon, 2010–2018 |
title_full_unstemmed |
Genetic characterization of African swine fever virus in Cameroon, 2010–2018 |
title_sort |
genetic characterization of african swine fever virus in cameroon, 2010–2018 |
url |
http://agritrop.cirad.fr/599896/ http://agritrop.cirad.fr/599896/1/599896.pdf |
work_keys_str_mv |
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