In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses
Background: Arthropod borne virus infections are the cause of severe emerging diseases. Among the diseases due to arboviruses, dengue (DEN) and Rift Valley fever (RVF) are in the top ten in the list of diseases responsible of severe human cases worldwide. Understanding the effects of viral infection on gene expression in competent vectors is a challenge for the development of early diagnostic tools and may enable researchers and policy makers to better anticipate outbreaks in the next future. Methods: In this study, alterations in gene expression across the entire Aedes aegypti genome during infection with DENV and RVFV were investigated in vitro at two time points of infection, the early phase (24 h) and the late phase (6 days) of infection using the RNA sequencing approach Results: A total of 10 upregulated genes that share a similar expression profile during infection with both viruses at early and late phases of infection were identified. Family B and D clip-domain serine proteases (CLIP) were clearly overrepresented as well as C-type lectins and transferrin. Conclusions: Our data highlight the presence of 10 viral genes upregulated in Ae. aegypti during infection. They may also be targeted in the case of the development of broad-spectrum anti-viral diagnostic tools focusing the mosquito vectors rather than the mammalian hosts as they may predict the emergence of outbreaks.
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Subjects: | S50 - Santé humaine, L72 - Organismes nuisibles des animaux, L10 - Génétique et amélioration des animaux, épidémiologie, virologie, expression des gènes, Aedes aegypti, vecteur de maladie, Virus de la fièvre de la vallée du Rift, arbovirus, transcriptome, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_8259, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_30482, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_16463, http://aims.fao.org/aos/agrovoc/c_331431, http://aims.fao.org/aos/agrovoc/c_620c5378, |
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dig-cirad-fr-5996822024-04-24T13:07:20Z http://agritrop.cirad.fr/599682/ http://agritrop.cirad.fr/599682/ In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses. Licciardi Séverine, Loire Etienne, Cardinale Eric, Gislard Marie, Dubois Emeric, Cetre-Sossah Catherine. 2020. Parasites and Vectors, 13 (1):395, 10 p.https://doi.org/10.1186/s13071-020-04253-5 <https://doi.org/10.1186/s13071-020-04253-5> In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses Licciardi, Séverine Loire, Etienne Cardinale, Eric Gislard, Marie Dubois, Emeric Cetre-Sossah, Catherine eng 2020 Parasites and Vectors S50 - Santé humaine L72 - Organismes nuisibles des animaux L10 - Génétique et amélioration des animaux épidémiologie virologie expression des gènes Aedes aegypti vecteur de maladie Virus de la fièvre de la vallée du Rift arbovirus transcriptome http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_30482 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_331431 http://aims.fao.org/aos/agrovoc/c_620c5378 Background: Arthropod borne virus infections are the cause of severe emerging diseases. Among the diseases due to arboviruses, dengue (DEN) and Rift Valley fever (RVF) are in the top ten in the list of diseases responsible of severe human cases worldwide. Understanding the effects of viral infection on gene expression in competent vectors is a challenge for the development of early diagnostic tools and may enable researchers and policy makers to better anticipate outbreaks in the next future. Methods: In this study, alterations in gene expression across the entire Aedes aegypti genome during infection with DENV and RVFV were investigated in vitro at two time points of infection, the early phase (24 h) and the late phase (6 days) of infection using the RNA sequencing approach Results: A total of 10 upregulated genes that share a similar expression profile during infection with both viruses at early and late phases of infection were identified. Family B and D clip-domain serine proteases (CLIP) were clearly overrepresented as well as C-type lectins and transferrin. Conclusions: Our data highlight the presence of 10 viral genes upregulated in Ae. aegypti during infection. They may also be targeted in the case of the development of broad-spectrum anti-viral diagnostic tools focusing the mosquito vectors rather than the mammalian hosts as they may predict the emergence of outbreaks. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/599682/1/599682.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1186/s13071-020-04253-5 10.1186/s13071-020-04253-5 info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-020-04253-5 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/s13071-020-04253-5 info:eu-repo/semantics/reference/purl/https://github.com/loire/CCS_RNAseq_analysis |
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S50 - Santé humaine L72 - Organismes nuisibles des animaux L10 - Génétique et amélioration des animaux épidémiologie virologie expression des gènes Aedes aegypti vecteur de maladie Virus de la fièvre de la vallée du Rift arbovirus transcriptome http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_30482 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_331431 http://aims.fao.org/aos/agrovoc/c_620c5378 S50 - Santé humaine L72 - Organismes nuisibles des animaux L10 - Génétique et amélioration des animaux épidémiologie virologie expression des gènes Aedes aegypti vecteur de maladie Virus de la fièvre de la vallée du Rift arbovirus transcriptome http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_30482 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_331431 http://aims.fao.org/aos/agrovoc/c_620c5378 |
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S50 - Santé humaine L72 - Organismes nuisibles des animaux L10 - Génétique et amélioration des animaux épidémiologie virologie expression des gènes Aedes aegypti vecteur de maladie Virus de la fièvre de la vallée du Rift arbovirus transcriptome http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_30482 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_331431 http://aims.fao.org/aos/agrovoc/c_620c5378 S50 - Santé humaine L72 - Organismes nuisibles des animaux L10 - Génétique et amélioration des animaux épidémiologie virologie expression des gènes Aedes aegypti vecteur de maladie Virus de la fièvre de la vallée du Rift arbovirus transcriptome http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_30482 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_331431 http://aims.fao.org/aos/agrovoc/c_620c5378 Licciardi, Séverine Loire, Etienne Cardinale, Eric Gislard, Marie Dubois, Emeric Cetre-Sossah, Catherine In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses |
description |
Background: Arthropod borne virus infections are the cause of severe emerging diseases. Among the diseases due to arboviruses, dengue (DEN) and Rift Valley fever (RVF) are in the top ten in the list of diseases responsible of severe human cases worldwide. Understanding the effects of viral infection on gene expression in competent vectors is a challenge for the development of early diagnostic tools and may enable researchers and policy makers to better anticipate outbreaks in the next future. Methods: In this study, alterations in gene expression across the entire Aedes aegypti genome during infection with DENV and RVFV were investigated in vitro at two time points of infection, the early phase (24 h) and the late phase (6 days) of infection using the RNA sequencing approach Results: A total of 10 upregulated genes that share a similar expression profile during infection with both viruses at early and late phases of infection were identified. Family B and D clip-domain serine proteases (CLIP) were clearly overrepresented as well as C-type lectins and transferrin. Conclusions: Our data highlight the presence of 10 viral genes upregulated in Ae. aegypti during infection. They may also be targeted in the case of the development of broad-spectrum anti-viral diagnostic tools focusing the mosquito vectors rather than the mammalian hosts as they may predict the emergence of outbreaks. |
format |
article |
topic_facet |
S50 - Santé humaine L72 - Organismes nuisibles des animaux L10 - Génétique et amélioration des animaux épidémiologie virologie expression des gènes Aedes aegypti vecteur de maladie Virus de la fièvre de la vallée du Rift arbovirus transcriptome http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_8259 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_30482 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_331431 http://aims.fao.org/aos/agrovoc/c_620c5378 |
author |
Licciardi, Séverine Loire, Etienne Cardinale, Eric Gislard, Marie Dubois, Emeric Cetre-Sossah, Catherine |
author_facet |
Licciardi, Séverine Loire, Etienne Cardinale, Eric Gislard, Marie Dubois, Emeric Cetre-Sossah, Catherine |
author_sort |
Licciardi, Séverine |
title |
In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses |
title_short |
In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses |
title_full |
In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses |
title_fullStr |
In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses |
title_full_unstemmed |
In vitro shared transcriptomic responses of Aedes aegypti to arboviral infections: Example of dengue and Rift Valley fever viruses |
title_sort |
in vitro shared transcriptomic responses of aedes aegypti to arboviral infections: example of dengue and rift valley fever viruses |
url |
http://agritrop.cirad.fr/599682/ http://agritrop.cirad.fr/599682/1/599682.pdf |
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