Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development
Moniliophthora perniciosa is a hemibiotrophic fungus that causes witches' broom disease in cacao (Theobroma cacao L.). The biotrophic fungal phase initiates the disease and is characterized by a monokaryotic mycelium, while the necrotrophic phase is characterized by a dikaryotic mycelium and leads to necrosis of infected tissues. A study of the necrotrophic phase was conducted on bran-based solid medium, which is the only medium that enables basidiocarp and basidiospore production. Six different fungal developmental phases were observed according to the mycelium colour or the organ produced: white, yellow, pink, dark pink, primordium and basidiocarp. In this study, we identified notable proteins in each phase, particularly those accumulated prior to basidiocarp formation. Proteins were analysed by proteomics; 2-D gels showed 300–550 spots. Statistically differentially accumulated spots were sequenced by mass spectrometry and 259 proteins were identified and categorized into nine functional classes. Proteins related to energy metabolism, protein folding and morphogenesis that were potentially involved in primordium and basidiocarp formation were identified; these proteins may represent useful candidates for further analysis related to the spread and pathogenesis of this fungus. To the best of our knowledge, this report describes the first proteomic analysis of the developmental phases of Moniliophthora perniciosa.
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Subjects: | H50 - Troubles divers des plantes, U60 - Sciences de la vie et de la Terre, maladie fongique, Theobroma cacao, mycelium, cycle de développement, basidiospore, protéomique, mycoprotéine, protéine, Moniliophthora, maladie du balai de sorcière, http://aims.fao.org/aos/agrovoc/c_11042, http://aims.fao.org/aos/agrovoc/c_7713, http://aims.fao.org/aos/agrovoc/c_5017, http://aims.fao.org/aos/agrovoc/c_4317, http://aims.fao.org/aos/agrovoc/c_35121, http://aims.fao.org/aos/agrovoc/c_38248aa9, http://aims.fao.org/aos/agrovoc/c_25437, http://aims.fao.org/aos/agrovoc/c_6259, http://aims.fao.org/aos/agrovoc/c_31727, http://aims.fao.org/aos/agrovoc/c_15380, |
Online Access: | http://agritrop.cirad.fr/599462/ http://agritrop.cirad.fr/599462/1/2021%20Gomes%20et%20al%20FGB.pdf |
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dig-cirad-fr-5994622024-01-29T03:49:38Z http://agritrop.cirad.fr/599462/ http://agritrop.cirad.fr/599462/ Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development. Santos Gomes Dayane, De Andrade Silva Edson Mario, De Andrade Rosa Emily Caroline, Silva Gualberto Nina Gabriela, de Jesus Souza Átila Monaliza, Santos Gesivaldo, Priminho Pirovani Carlos, Micheli Fabienne. 2021. Fungal Genetics and Biology, 157:103635, 16 p.https://doi.org/10.1016/j.fgb.2021.103635 <https://doi.org/10.1016/j.fgb.2021.103635> Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development Santos Gomes, Dayane De Andrade Silva, Edson Mario De Andrade Rosa, Emily Caroline Silva Gualberto, Nina Gabriela de Jesus Souza, Átila Monaliza Santos, Gesivaldo Priminho Pirovani, Carlos Micheli, Fabienne eng 2021 Fungal Genetics and Biology H50 - Troubles divers des plantes U60 - Sciences de la vie et de la Terre maladie fongique Theobroma cacao mycelium cycle de développement basidiospore protéomique mycoprotéine protéine Moniliophthora maladie du balai de sorcière http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_5017 http://aims.fao.org/aos/agrovoc/c_4317 http://aims.fao.org/aos/agrovoc/c_35121 http://aims.fao.org/aos/agrovoc/c_38248aa9 http://aims.fao.org/aos/agrovoc/c_25437 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_31727 http://aims.fao.org/aos/agrovoc/c_15380 Moniliophthora perniciosa is a hemibiotrophic fungus that causes witches' broom disease in cacao (Theobroma cacao L.). The biotrophic fungal phase initiates the disease and is characterized by a monokaryotic mycelium, while the necrotrophic phase is characterized by a dikaryotic mycelium and leads to necrosis of infected tissues. A study of the necrotrophic phase was conducted on bran-based solid medium, which is the only medium that enables basidiocarp and basidiospore production. Six different fungal developmental phases were observed according to the mycelium colour or the organ produced: white, yellow, pink, dark pink, primordium and basidiocarp. In this study, we identified notable proteins in each phase, particularly those accumulated prior to basidiocarp formation. Proteins were analysed by proteomics; 2-D gels showed 300–550 spots. Statistically differentially accumulated spots were sequenced by mass spectrometry and 259 proteins were identified and categorized into nine functional classes. Proteins related to energy metabolism, protein folding and morphogenesis that were potentially involved in primordium and basidiocarp formation were identified; these proteins may represent useful candidates for further analysis related to the spread and pathogenesis of this fungus. To the best of our knowledge, this report describes the first proteomic analysis of the developmental phases of Moniliophthora perniciosa. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/599462/1/2021%20Gomes%20et%20al%20FGB.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.fgb.2021.103635 10.1016/j.fgb.2021.103635 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fgb.2021.103635 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.fgb.2021.103635 |
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H50 - Troubles divers des plantes U60 - Sciences de la vie et de la Terre maladie fongique Theobroma cacao mycelium cycle de développement basidiospore protéomique mycoprotéine protéine Moniliophthora maladie du balai de sorcière http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_5017 http://aims.fao.org/aos/agrovoc/c_4317 http://aims.fao.org/aos/agrovoc/c_35121 http://aims.fao.org/aos/agrovoc/c_38248aa9 http://aims.fao.org/aos/agrovoc/c_25437 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_31727 http://aims.fao.org/aos/agrovoc/c_15380 H50 - Troubles divers des plantes U60 - Sciences de la vie et de la Terre maladie fongique Theobroma cacao mycelium cycle de développement basidiospore protéomique mycoprotéine protéine Moniliophthora maladie du balai de sorcière http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_5017 http://aims.fao.org/aos/agrovoc/c_4317 http://aims.fao.org/aos/agrovoc/c_35121 http://aims.fao.org/aos/agrovoc/c_38248aa9 http://aims.fao.org/aos/agrovoc/c_25437 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_31727 http://aims.fao.org/aos/agrovoc/c_15380 |
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H50 - Troubles divers des plantes U60 - Sciences de la vie et de la Terre maladie fongique Theobroma cacao mycelium cycle de développement basidiospore protéomique mycoprotéine protéine Moniliophthora maladie du balai de sorcière http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_5017 http://aims.fao.org/aos/agrovoc/c_4317 http://aims.fao.org/aos/agrovoc/c_35121 http://aims.fao.org/aos/agrovoc/c_38248aa9 http://aims.fao.org/aos/agrovoc/c_25437 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_31727 http://aims.fao.org/aos/agrovoc/c_15380 H50 - Troubles divers des plantes U60 - Sciences de la vie et de la Terre maladie fongique Theobroma cacao mycelium cycle de développement basidiospore protéomique mycoprotéine protéine Moniliophthora maladie du balai de sorcière http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_5017 http://aims.fao.org/aos/agrovoc/c_4317 http://aims.fao.org/aos/agrovoc/c_35121 http://aims.fao.org/aos/agrovoc/c_38248aa9 http://aims.fao.org/aos/agrovoc/c_25437 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_31727 http://aims.fao.org/aos/agrovoc/c_15380 Santos Gomes, Dayane De Andrade Silva, Edson Mario De Andrade Rosa, Emily Caroline Silva Gualberto, Nina Gabriela de Jesus Souza, Átila Monaliza Santos, Gesivaldo Priminho Pirovani, Carlos Micheli, Fabienne Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development |
description |
Moniliophthora perniciosa is a hemibiotrophic fungus that causes witches' broom disease in cacao (Theobroma cacao L.). The biotrophic fungal phase initiates the disease and is characterized by a monokaryotic mycelium, while the necrotrophic phase is characterized by a dikaryotic mycelium and leads to necrosis of infected tissues. A study of the necrotrophic phase was conducted on bran-based solid medium, which is the only medium that enables basidiocarp and basidiospore production. Six different fungal developmental phases were observed according to the mycelium colour or the organ produced: white, yellow, pink, dark pink, primordium and basidiocarp. In this study, we identified notable proteins in each phase, particularly those accumulated prior to basidiocarp formation. Proteins were analysed by proteomics; 2-D gels showed 300–550 spots. Statistically differentially accumulated spots were sequenced by mass spectrometry and 259 proteins were identified and categorized into nine functional classes. Proteins related to energy metabolism, protein folding and morphogenesis that were potentially involved in primordium and basidiocarp formation were identified; these proteins may represent useful candidates for further analysis related to the spread and pathogenesis of this fungus. To the best of our knowledge, this report describes the first proteomic analysis of the developmental phases of Moniliophthora perniciosa. |
format |
article |
topic_facet |
H50 - Troubles divers des plantes U60 - Sciences de la vie et de la Terre maladie fongique Theobroma cacao mycelium cycle de développement basidiospore protéomique mycoprotéine protéine Moniliophthora maladie du balai de sorcière http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_5017 http://aims.fao.org/aos/agrovoc/c_4317 http://aims.fao.org/aos/agrovoc/c_35121 http://aims.fao.org/aos/agrovoc/c_38248aa9 http://aims.fao.org/aos/agrovoc/c_25437 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_31727 http://aims.fao.org/aos/agrovoc/c_15380 |
author |
Santos Gomes, Dayane De Andrade Silva, Edson Mario De Andrade Rosa, Emily Caroline Silva Gualberto, Nina Gabriela de Jesus Souza, Átila Monaliza Santos, Gesivaldo Priminho Pirovani, Carlos Micheli, Fabienne |
author_facet |
Santos Gomes, Dayane De Andrade Silva, Edson Mario De Andrade Rosa, Emily Caroline Silva Gualberto, Nina Gabriela de Jesus Souza, Átila Monaliza Santos, Gesivaldo Priminho Pirovani, Carlos Micheli, Fabienne |
author_sort |
Santos Gomes, Dayane |
title |
Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development |
title_short |
Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development |
title_full |
Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development |
title_fullStr |
Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development |
title_full_unstemmed |
Identification of a key protein set involved in Moniliophthora perniciosa necrotrophic mycelium and basidiocarp development |
title_sort |
identification of a key protein set involved in moniliophthora perniciosa necrotrophic mycelium and basidiocarp development |
url |
http://agritrop.cirad.fr/599462/ http://agritrop.cirad.fr/599462/1/2021%20Gomes%20et%20al%20FGB.pdf |
work_keys_str_mv |
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1792500243816775680 |