Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime
Huanglongbing (HLB) is presently a major threat to the citrus industry. Because of this disease, millions of trees are currently dying worldwide. The putative causal agent is a motile bacteria belonging to Candidatus Liberibacter spp., which is transmitted by psyllids. The bacteria is responsible for the synthesis of callose at the phloem sieve plate, leading to the obstruction of the pores that provide connections between adjacent sieve elements, thus limiting the symplastic transport of the sugars and starches synthesized in leaves to the other plant organs. The Persian triploid lime (Citrus latifolia) is one of the most HLB-tolerant citrus varieties, but the determinants associated with the tolerance are still unknown. HLB-infected diploid Mexican lime (Citrus aurantiifolia) and Persian lime were investigated. The leaf petiole was analyzed using scanning electron microscopy (SEM) to observe callose deposition at the phloem sieve plate. Leaf starch contents and detoxification enzyme activities were investigated. In the field, Persian lime leaves present more limited symptoms due to HLB than the Mexican lime leaves do. Photosynthesis, stomatal conductance, and transpiration decreased compared with control plants, but values remained greater in the Persian than in the Mexican lime. Analysis of the petiole sieve plate in control petiole samples showed that pores were approximately 1.8-fold larger in the Persian than in the Mexican lime. SEM analyses of petiole samples of symptomatic leaves showed the important deposition of callose into pores of Mexican and Persian limes, whereas biochemical analyses revealed better detoxification in Persian limes than in Mexican limes. Moreover, SEM analyses of infected petiole samples of asymptomatic leaves showed much larger callose depositions into the Mexican lime pores than in the Persian lime pores, whereas biochemical traits revealed much better behavior in Persian limes than in Mexican limes. Our results reveal that polyploids present specific behaviors associated with important physiological and biochemical determinants that may explain the better tolerance of the Persian lime against HLB compared with the Mexican lime.
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Subjects: | H20 - Maladies des plantes, F60 - Physiologie et biochimie végétale, maladie des plantes, résistance aux maladies, détoxification, lime, Citrus aurantiifolia, polyploïdie, physiologie végétale, bactérie gram négatif, http://aims.fao.org/aos/agrovoc/c_5962, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_2219, http://aims.fao.org/aos/agrovoc/c_4341, http://aims.fao.org/aos/agrovoc/c_1638, http://aims.fao.org/aos/agrovoc/c_6094, http://aims.fao.org/aos/agrovoc/c_25189, http://aims.fao.org/aos/agrovoc/c_3352, |
Online Access: | http://agritrop.cirad.fr/599304/ http://agritrop.cirad.fr/599304/1/fpls-12-685679.pdf |
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H20 - Maladies des plantes F60 - Physiologie et biochimie végétale maladie des plantes résistance aux maladies détoxification lime Citrus aurantiifolia polyploïdie physiologie végétale bactérie gram négatif http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2219 http://aims.fao.org/aos/agrovoc/c_4341 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_6094 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3352 H20 - Maladies des plantes F60 - Physiologie et biochimie végétale maladie des plantes résistance aux maladies détoxification lime Citrus aurantiifolia polyploïdie physiologie végétale bactérie gram négatif http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2219 http://aims.fao.org/aos/agrovoc/c_4341 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_6094 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3352 |
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H20 - Maladies des plantes F60 - Physiologie et biochimie végétale maladie des plantes résistance aux maladies détoxification lime Citrus aurantiifolia polyploïdie physiologie végétale bactérie gram négatif http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2219 http://aims.fao.org/aos/agrovoc/c_4341 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_6094 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3352 H20 - Maladies des plantes F60 - Physiologie et biochimie végétale maladie des plantes résistance aux maladies détoxification lime Citrus aurantiifolia polyploïdie physiologie végétale bactérie gram négatif http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2219 http://aims.fao.org/aos/agrovoc/c_4341 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_6094 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3352 Sivager, Gary Calvez, Lény Bruyère, Saturnin Boisne-Noc, Rosiane Brat, Pierre Gros, Olivier Ollitrault, Patrick Morillon, Raphaël Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime |
description |
Huanglongbing (HLB) is presently a major threat to the citrus industry. Because of this disease, millions of trees are currently dying worldwide. The putative causal agent is a motile bacteria belonging to Candidatus Liberibacter spp., which is transmitted by psyllids. The bacteria is responsible for the synthesis of callose at the phloem sieve plate, leading to the obstruction of the pores that provide connections between adjacent sieve elements, thus limiting the symplastic transport of the sugars and starches synthesized in leaves to the other plant organs. The Persian triploid lime (Citrus latifolia) is one of the most HLB-tolerant citrus varieties, but the determinants associated with the tolerance are still unknown. HLB-infected diploid Mexican lime (Citrus aurantiifolia) and Persian lime were investigated. The leaf petiole was analyzed using scanning electron microscopy (SEM) to observe callose deposition at the phloem sieve plate. Leaf starch contents and detoxification enzyme activities were investigated. In the field, Persian lime leaves present more limited symptoms due to HLB than the Mexican lime leaves do. Photosynthesis, stomatal conductance, and transpiration decreased compared with control plants, but values remained greater in the Persian than in the Mexican lime. Analysis of the petiole sieve plate in control petiole samples showed that pores were approximately 1.8-fold larger in the Persian than in the Mexican lime. SEM analyses of petiole samples of symptomatic leaves showed the important deposition of callose into pores of Mexican and Persian limes, whereas biochemical analyses revealed better detoxification in Persian limes than in Mexican limes. Moreover, SEM analyses of infected petiole samples of asymptomatic leaves showed much larger callose depositions into the Mexican lime pores than in the Persian lime pores, whereas biochemical traits revealed much better behavior in Persian limes than in Mexican limes. Our results reveal that polyploids present specific behaviors associated with important physiological and biochemical determinants that may explain the better tolerance of the Persian lime against HLB compared with the Mexican lime. |
format |
article |
topic_facet |
H20 - Maladies des plantes F60 - Physiologie et biochimie végétale maladie des plantes résistance aux maladies détoxification lime Citrus aurantiifolia polyploïdie physiologie végétale bactérie gram négatif http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2219 http://aims.fao.org/aos/agrovoc/c_4341 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_6094 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3352 |
author |
Sivager, Gary Calvez, Lény Bruyère, Saturnin Boisne-Noc, Rosiane Brat, Pierre Gros, Olivier Ollitrault, Patrick Morillon, Raphaël |
author_facet |
Sivager, Gary Calvez, Lény Bruyère, Saturnin Boisne-Noc, Rosiane Brat, Pierre Gros, Olivier Ollitrault, Patrick Morillon, Raphaël |
author_sort |
Sivager, Gary |
title |
Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime |
title_short |
Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime |
title_full |
Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime |
title_fullStr |
Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime |
title_full_unstemmed |
Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime |
title_sort |
specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the persian lime compared with the mexican lime |
url |
http://agritrop.cirad.fr/599304/ http://agritrop.cirad.fr/599304/1/fpls-12-685679.pdf |
work_keys_str_mv |
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dig-cirad-fr-5993042024-01-29T03:47:01Z http://agritrop.cirad.fr/599304/ http://agritrop.cirad.fr/599304/ Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime. Sivager Gary, Calvez Lény, Bruyère Saturnin, Boisne-Noc Rosiane, Brat Pierre, Gros Olivier, Ollitrault Patrick, Morillon Raphaël. 2021. Frontiers in Plant Science, 12:685679, 14 p.https://doi.org/10.3389/fpls.2021.685679 <https://doi.org/10.3389/fpls.2021.685679> Specific physiological and anatomical traits associated with polyploidy and better detoxification processes contribute to improved huanglongbing tolerance of the Persian lime Compared with the Mexican lime Sivager, Gary Calvez, Lény Bruyère, Saturnin Boisne-Noc, Rosiane Brat, Pierre Gros, Olivier Ollitrault, Patrick Morillon, Raphaël eng 2021 Frontiers in Plant Science H20 - Maladies des plantes F60 - Physiologie et biochimie végétale maladie des plantes résistance aux maladies détoxification lime Citrus aurantiifolia polyploïdie physiologie végétale bactérie gram négatif http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2219 http://aims.fao.org/aos/agrovoc/c_4341 http://aims.fao.org/aos/agrovoc/c_1638 http://aims.fao.org/aos/agrovoc/c_6094 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3352 Huanglongbing (HLB) is presently a major threat to the citrus industry. Because of this disease, millions of trees are currently dying worldwide. The putative causal agent is a motile bacteria belonging to Candidatus Liberibacter spp., which is transmitted by psyllids. The bacteria is responsible for the synthesis of callose at the phloem sieve plate, leading to the obstruction of the pores that provide connections between adjacent sieve elements, thus limiting the symplastic transport of the sugars and starches synthesized in leaves to the other plant organs. The Persian triploid lime (Citrus latifolia) is one of the most HLB-tolerant citrus varieties, but the determinants associated with the tolerance are still unknown. HLB-infected diploid Mexican lime (Citrus aurantiifolia) and Persian lime were investigated. The leaf petiole was analyzed using scanning electron microscopy (SEM) to observe callose deposition at the phloem sieve plate. Leaf starch contents and detoxification enzyme activities were investigated. In the field, Persian lime leaves present more limited symptoms due to HLB than the Mexican lime leaves do. Photosynthesis, stomatal conductance, and transpiration decreased compared with control plants, but values remained greater in the Persian than in the Mexican lime. Analysis of the petiole sieve plate in control petiole samples showed that pores were approximately 1.8-fold larger in the Persian than in the Mexican lime. SEM analyses of petiole samples of symptomatic leaves showed the important deposition of callose into pores of Mexican and Persian limes, whereas biochemical analyses revealed better detoxification in Persian limes than in Mexican limes. Moreover, SEM analyses of infected petiole samples of asymptomatic leaves showed much larger callose depositions into the Mexican lime pores than in the Persian lime pores, whereas biochemical traits revealed much better behavior in Persian limes than in Mexican limes. Our results reveal that polyploids present specific behaviors associated with important physiological and biochemical determinants that may explain the better tolerance of the Persian lime against HLB compared with the Mexican lime. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/599304/1/fpls-12-685679.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3389/fpls.2021.685679 10.3389/fpls.2021.685679 info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2021.685679 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3389/fpls.2021.685679 info:eu-repo/grantAgreement/EC/H2020/727459//(EU) Insect-borne prokaryote-associated diseases in tropical and subtropical perennial crops/TROPICSAFE |