In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances
Animal African Trypanosomosis (AAT or Nagana) is a severe vector-borne disease caused by protozoan parasites belonging to the Trypanosomatidae family and is usually cyclically transmitted by blood-sucking tsetse flies. AAT remains a major problem in sub-Saharan Africa. Among the main AAT causative agents, Trypanosoma congolense (T. congolense or Tc) is one of the most important trypanosome species, in terms of economic and animal health impacts, infecting cattle and a wide range of animal hosts as well. To advance in AAT prevention and control, it is essential to better understand trypanosome biology and pathogenesis using bloodstream form (BSF) in vitro culture. The in vitro cultivation of T. congolense IL3000 BSF strain is already well established and widely used in research studies and drug activity assays. However, it may probably no longer truly reflect the reality of field trypanosome strains, due to decades of use and subsequent modifications. Here, we propose a novel culture protocol that supports the long-term in vitro growth of the animal-infective BSFs of three Savannah and Forest types of T. congolense strains, including T. congolense clone IL1180, which is not only a field strain but also a commonly-used reference strain in experimental animal assays. We established a homemade culture medium which made it possible to sustain T. congolense IL1180 growth from infected mouse blood for 18 days in axenic conditions. Moreover, we developed an efficient freezing/thawing system that allowed, for the first time, T. congolense IL1180 BSF growth within 30 days after thawing. Our results on T. congolense adaptation to in vitro culture are encouraging for future gene studies using new molecular tools or for new therapeutic drug assays.
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Subjects: | L73 - Maladies des animaux, trypanosomose africaine, Trypanosoma congolense, culture in vitro, épidémiologie, circulation sanguine, maladie des animaux, maladie transmise par vecteur, http://aims.fao.org/aos/agrovoc/c_35903, http://aims.fao.org/aos/agrovoc/c_27401, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_961, http://aims.fao.org/aos/agrovoc/c_426, http://aims.fao.org/aos/agrovoc/c_34142, |
Online Access: | http://agritrop.cirad.fr/599115/ http://agritrop.cirad.fr/599115/1/I.%20Chantal%20et%20al%202021%20In%20vitro%20cultivation%20of%20Trypanosoma%20congolense%20bloodstream%20forms%20State%20of%20art%20and%20advances.pdf |
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dig-cirad-fr-5991152024-01-29T03:44:48Z http://agritrop.cirad.fr/599115/ http://agritrop.cirad.fr/599115/ In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances. Chantal Isabelle, Minet Cécile, Berthier David. 2021. Veterinary Parasitology, 299:109567, 9 p.https://doi.org/10.1016/j.vetpar.2021.109567 <https://doi.org/10.1016/j.vetpar.2021.109567> In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances Chantal, Isabelle Minet, Cécile Berthier, David eng 2021 Veterinary Parasitology L73 - Maladies des animaux trypanosomose africaine Trypanosoma congolense culture in vitro épidémiologie circulation sanguine maladie des animaux maladie transmise par vecteur http://aims.fao.org/aos/agrovoc/c_35903 http://aims.fao.org/aos/agrovoc/c_27401 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_961 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_34142 Animal African Trypanosomosis (AAT or Nagana) is a severe vector-borne disease caused by protozoan parasites belonging to the Trypanosomatidae family and is usually cyclically transmitted by blood-sucking tsetse flies. AAT remains a major problem in sub-Saharan Africa. Among the main AAT causative agents, Trypanosoma congolense (T. congolense or Tc) is one of the most important trypanosome species, in terms of economic and animal health impacts, infecting cattle and a wide range of animal hosts as well. To advance in AAT prevention and control, it is essential to better understand trypanosome biology and pathogenesis using bloodstream form (BSF) in vitro culture. The in vitro cultivation of T. congolense IL3000 BSF strain is already well established and widely used in research studies and drug activity assays. However, it may probably no longer truly reflect the reality of field trypanosome strains, due to decades of use and subsequent modifications. Here, we propose a novel culture protocol that supports the long-term in vitro growth of the animal-infective BSFs of three Savannah and Forest types of T. congolense strains, including T. congolense clone IL1180, which is not only a field strain but also a commonly-used reference strain in experimental animal assays. We established a homemade culture medium which made it possible to sustain T. congolense IL1180 growth from infected mouse blood for 18 days in axenic conditions. Moreover, we developed an efficient freezing/thawing system that allowed, for the first time, T. congolense IL1180 BSF growth within 30 days after thawing. Our results on T. congolense adaptation to in vitro culture are encouraging for future gene studies using new molecular tools or for new therapeutic drug assays. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/599115/1/I.%20Chantal%20et%20al%202021%20In%20vitro%20cultivation%20of%20Trypanosoma%20congolense%20bloodstream%20forms%20State%20of%20art%20and%20advances.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.vetpar.2021.109567 10.1016/j.vetpar.2021.109567 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2021.109567 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.vetpar.2021.109567 |
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L73 - Maladies des animaux trypanosomose africaine Trypanosoma congolense culture in vitro épidémiologie circulation sanguine maladie des animaux maladie transmise par vecteur http://aims.fao.org/aos/agrovoc/c_35903 http://aims.fao.org/aos/agrovoc/c_27401 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_961 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_34142 L73 - Maladies des animaux trypanosomose africaine Trypanosoma congolense culture in vitro épidémiologie circulation sanguine maladie des animaux maladie transmise par vecteur http://aims.fao.org/aos/agrovoc/c_35903 http://aims.fao.org/aos/agrovoc/c_27401 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_961 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_34142 |
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L73 - Maladies des animaux trypanosomose africaine Trypanosoma congolense culture in vitro épidémiologie circulation sanguine maladie des animaux maladie transmise par vecteur http://aims.fao.org/aos/agrovoc/c_35903 http://aims.fao.org/aos/agrovoc/c_27401 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_961 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_34142 L73 - Maladies des animaux trypanosomose africaine Trypanosoma congolense culture in vitro épidémiologie circulation sanguine maladie des animaux maladie transmise par vecteur http://aims.fao.org/aos/agrovoc/c_35903 http://aims.fao.org/aos/agrovoc/c_27401 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_961 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_34142 Chantal, Isabelle Minet, Cécile Berthier, David In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances |
description |
Animal African Trypanosomosis (AAT or Nagana) is a severe vector-borne disease caused by protozoan parasites belonging to the Trypanosomatidae family and is usually cyclically transmitted by blood-sucking tsetse flies. AAT remains a major problem in sub-Saharan Africa. Among the main AAT causative agents, Trypanosoma congolense (T. congolense or Tc) is one of the most important trypanosome species, in terms of economic and animal health impacts, infecting cattle and a wide range of animal hosts as well. To advance in AAT prevention and control, it is essential to better understand trypanosome biology and pathogenesis using bloodstream form (BSF) in vitro culture. The in vitro cultivation of T. congolense IL3000 BSF strain is already well established and widely used in research studies and drug activity assays. However, it may probably no longer truly reflect the reality of field trypanosome strains, due to decades of use and subsequent modifications. Here, we propose a novel culture protocol that supports the long-term in vitro growth of the animal-infective BSFs of three Savannah and Forest types of T. congolense strains, including T. congolense clone IL1180, which is not only a field strain but also a commonly-used reference strain in experimental animal assays. We established a homemade culture medium which made it possible to sustain T. congolense IL1180 growth from infected mouse blood for 18 days in axenic conditions. Moreover, we developed an efficient freezing/thawing system that allowed, for the first time, T. congolense IL1180 BSF growth within 30 days after thawing. Our results on T. congolense adaptation to in vitro culture are encouraging for future gene studies using new molecular tools or for new therapeutic drug assays. |
format |
article |
topic_facet |
L73 - Maladies des animaux trypanosomose africaine Trypanosoma congolense culture in vitro épidémiologie circulation sanguine maladie des animaux maladie transmise par vecteur http://aims.fao.org/aos/agrovoc/c_35903 http://aims.fao.org/aos/agrovoc/c_27401 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_961 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_34142 |
author |
Chantal, Isabelle Minet, Cécile Berthier, David |
author_facet |
Chantal, Isabelle Minet, Cécile Berthier, David |
author_sort |
Chantal, Isabelle |
title |
In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances |
title_short |
In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances |
title_full |
In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances |
title_fullStr |
In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances |
title_full_unstemmed |
In vitro cultivation of Trypanosoma congolense bloodstream forms: State of art and advances |
title_sort |
in vitro cultivation of trypanosoma congolense bloodstream forms: state of art and advances |
url |
http://agritrop.cirad.fr/599115/ http://agritrop.cirad.fr/599115/1/I.%20Chantal%20et%20al%202021%20In%20vitro%20cultivation%20of%20Trypanosoma%20congolense%20bloodstream%20forms%20State%20of%20art%20and%20advances.pdf |
work_keys_str_mv |
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