Early transcriptional response to gravistimulation in poplar without phototropic confounding factors
In response to gravistimulation under anisotropic light, tree stems showing an active cambium produce reaction wood that redirects the axis of the trees. Several studies have described transcriptomic or proteomic models of reaction wood relative to the opposite wood. However, the mechanisms leading to the formation of reaction wood are difficult to decipher because so many environmental factors can induce various signalling pathways leading to this developmental reprogramming. Using an innovative isotropic device where the phototropic response does not interfere with gravistimulation we characterized the early molecular responses occurring in the stem of poplar after gravistimulation in an isotropic environment, and without deformation of the stem. After 30 min tilting at 35° under anisotropic light, we collected the upper and lower xylems from the inclined stems. Controls were collected from vertical stems. We used a microarray approach to identify differentially expressed transcripts. High-throughput real-time PCR allowed a kinetic experiment at 0, 30, 120 and 180 min after tilting at 35°, with candidate genes. We identified 668 differentially expressed transcripts, from which we selected 153 candidates for additional Fluidigm qPCR assessment. Five candidate co-expression gene clusters have been identified after the kinetic monitoring of the expression of candidate genes. Gene ontology analyses indicate that molecular reprogramming of processes such as 'wood cell expansion', 'cell wall reorganization' and 'programmed cell death' occur as early as 30 min after gravistimulation. Of note is that the change in the expression of different genes involves a fine regulation of gibberellin and brassinosteroid pathways as well as flavonoid and phosphoinositide pathways. Our experimental set-up allowed the identification of genes regulated in early gravitropic response without the bias introduced by phototropic and stem bending responses.
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Subjects: | F62 - Physiologie végétale - Croissance et développement, F30 - Génétique et amélioration des plantes, gravitropisme, bois de réaction, paroi cellulaire, Populus alba, Populus tremula, xylème, port de la plante, tige, http://aims.fao.org/aos/agrovoc/c_11127, http://aims.fao.org/aos/agrovoc/c_24890, http://aims.fao.org/aos/agrovoc/c_1415, http://aims.fao.org/aos/agrovoc/c_26632, http://aims.fao.org/aos/agrovoc/c_23967, http://aims.fao.org/aos/agrovoc/c_8471, http://aims.fao.org/aos/agrovoc/c_5969, http://aims.fao.org/aos/agrovoc/c_7390, |
Online Access: | http://agritrop.cirad.fr/597754/ http://agritrop.cirad.fr/597754/1/plaa071.pdf |
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dig-cirad-fr-5977542024-01-29T03:22:57Z http://agritrop.cirad.fr/597754/ http://agritrop.cirad.fr/597754/ Early transcriptional response to gravistimulation in poplar without phototropic confounding factors. Lopez David, Franchel Jérôme, Venisse Jean-Stéphane, Drevet Patricia, Label Philippe, Coutand Catherine, Roeckel-Drevet Patricia. 2021. AoB Plants, 13 (1), 17 p.https://doi.org/10.1093/aobpla/plaa071 <https://doi.org/10.1093/aobpla/plaa071> Early transcriptional response to gravistimulation in poplar without phototropic confounding factors Lopez, David Franchel, Jérôme Venisse, Jean-Stéphane Drevet, Patricia Label, Philippe Coutand, Catherine Roeckel-Drevet, Patricia eng 2021 AoB Plants F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes gravitropisme bois de réaction paroi cellulaire Populus alba Populus tremula xylème port de la plante tige http://aims.fao.org/aos/agrovoc/c_11127 http://aims.fao.org/aos/agrovoc/c_24890 http://aims.fao.org/aos/agrovoc/c_1415 http://aims.fao.org/aos/agrovoc/c_26632 http://aims.fao.org/aos/agrovoc/c_23967 http://aims.fao.org/aos/agrovoc/c_8471 http://aims.fao.org/aos/agrovoc/c_5969 http://aims.fao.org/aos/agrovoc/c_7390 In response to gravistimulation under anisotropic light, tree stems showing an active cambium produce reaction wood that redirects the axis of the trees. Several studies have described transcriptomic or proteomic models of reaction wood relative to the opposite wood. However, the mechanisms leading to the formation of reaction wood are difficult to decipher because so many environmental factors can induce various signalling pathways leading to this developmental reprogramming. Using an innovative isotropic device where the phototropic response does not interfere with gravistimulation we characterized the early molecular responses occurring in the stem of poplar after gravistimulation in an isotropic environment, and without deformation of the stem. After 30 min tilting at 35° under anisotropic light, we collected the upper and lower xylems from the inclined stems. Controls were collected from vertical stems. We used a microarray approach to identify differentially expressed transcripts. High-throughput real-time PCR allowed a kinetic experiment at 0, 30, 120 and 180 min after tilting at 35°, with candidate genes. We identified 668 differentially expressed transcripts, from which we selected 153 candidates for additional Fluidigm qPCR assessment. Five candidate co-expression gene clusters have been identified after the kinetic monitoring of the expression of candidate genes. Gene ontology analyses indicate that molecular reprogramming of processes such as 'wood cell expansion', 'cell wall reorganization' and 'programmed cell death' occur as early as 30 min after gravistimulation. Of note is that the change in the expression of different genes involves a fine regulation of gibberellin and brassinosteroid pathways as well as flavonoid and phosphoinositide pathways. Our experimental set-up allowed the identification of genes regulated in early gravitropic response without the bias introduced by phototropic and stem bending responses. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/597754/1/plaa071.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1093/aobpla/plaa071 10.1093/aobpla/plaa071 info:eu-repo/semantics/altIdentifier/doi/10.1093/aobpla/plaa071 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1093/aobpla/plaa071 |
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F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes gravitropisme bois de réaction paroi cellulaire Populus alba Populus tremula xylème port de la plante tige http://aims.fao.org/aos/agrovoc/c_11127 http://aims.fao.org/aos/agrovoc/c_24890 http://aims.fao.org/aos/agrovoc/c_1415 http://aims.fao.org/aos/agrovoc/c_26632 http://aims.fao.org/aos/agrovoc/c_23967 http://aims.fao.org/aos/agrovoc/c_8471 http://aims.fao.org/aos/agrovoc/c_5969 http://aims.fao.org/aos/agrovoc/c_7390 F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes gravitropisme bois de réaction paroi cellulaire Populus alba Populus tremula xylème port de la plante tige http://aims.fao.org/aos/agrovoc/c_11127 http://aims.fao.org/aos/agrovoc/c_24890 http://aims.fao.org/aos/agrovoc/c_1415 http://aims.fao.org/aos/agrovoc/c_26632 http://aims.fao.org/aos/agrovoc/c_23967 http://aims.fao.org/aos/agrovoc/c_8471 http://aims.fao.org/aos/agrovoc/c_5969 http://aims.fao.org/aos/agrovoc/c_7390 |
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F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes gravitropisme bois de réaction paroi cellulaire Populus alba Populus tremula xylème port de la plante tige http://aims.fao.org/aos/agrovoc/c_11127 http://aims.fao.org/aos/agrovoc/c_24890 http://aims.fao.org/aos/agrovoc/c_1415 http://aims.fao.org/aos/agrovoc/c_26632 http://aims.fao.org/aos/agrovoc/c_23967 http://aims.fao.org/aos/agrovoc/c_8471 http://aims.fao.org/aos/agrovoc/c_5969 http://aims.fao.org/aos/agrovoc/c_7390 F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes gravitropisme bois de réaction paroi cellulaire Populus alba Populus tremula xylème port de la plante tige http://aims.fao.org/aos/agrovoc/c_11127 http://aims.fao.org/aos/agrovoc/c_24890 http://aims.fao.org/aos/agrovoc/c_1415 http://aims.fao.org/aos/agrovoc/c_26632 http://aims.fao.org/aos/agrovoc/c_23967 http://aims.fao.org/aos/agrovoc/c_8471 http://aims.fao.org/aos/agrovoc/c_5969 http://aims.fao.org/aos/agrovoc/c_7390 Lopez, David Franchel, Jérôme Venisse, Jean-Stéphane Drevet, Patricia Label, Philippe Coutand, Catherine Roeckel-Drevet, Patricia Early transcriptional response to gravistimulation in poplar without phototropic confounding factors |
description |
In response to gravistimulation under anisotropic light, tree stems showing an active cambium produce reaction wood that redirects the axis of the trees. Several studies have described transcriptomic or proteomic models of reaction wood relative to the opposite wood. However, the mechanisms leading to the formation of reaction wood are difficult to decipher because so many environmental factors can induce various signalling pathways leading to this developmental reprogramming. Using an innovative isotropic device where the phototropic response does not interfere with gravistimulation we characterized the early molecular responses occurring in the stem of poplar after gravistimulation in an isotropic environment, and without deformation of the stem. After 30 min tilting at 35° under anisotropic light, we collected the upper and lower xylems from the inclined stems. Controls were collected from vertical stems. We used a microarray approach to identify differentially expressed transcripts. High-throughput real-time PCR allowed a kinetic experiment at 0, 30, 120 and 180 min after tilting at 35°, with candidate genes. We identified 668 differentially expressed transcripts, from which we selected 153 candidates for additional Fluidigm qPCR assessment. Five candidate co-expression gene clusters have been identified after the kinetic monitoring of the expression of candidate genes. Gene ontology analyses indicate that molecular reprogramming of processes such as 'wood cell expansion', 'cell wall reorganization' and 'programmed cell death' occur as early as 30 min after gravistimulation. Of note is that the change in the expression of different genes involves a fine regulation of gibberellin and brassinosteroid pathways as well as flavonoid and phosphoinositide pathways. Our experimental set-up allowed the identification of genes regulated in early gravitropic response without the bias introduced by phototropic and stem bending responses. |
format |
article |
topic_facet |
F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes gravitropisme bois de réaction paroi cellulaire Populus alba Populus tremula xylème port de la plante tige http://aims.fao.org/aos/agrovoc/c_11127 http://aims.fao.org/aos/agrovoc/c_24890 http://aims.fao.org/aos/agrovoc/c_1415 http://aims.fao.org/aos/agrovoc/c_26632 http://aims.fao.org/aos/agrovoc/c_23967 http://aims.fao.org/aos/agrovoc/c_8471 http://aims.fao.org/aos/agrovoc/c_5969 http://aims.fao.org/aos/agrovoc/c_7390 |
author |
Lopez, David Franchel, Jérôme Venisse, Jean-Stéphane Drevet, Patricia Label, Philippe Coutand, Catherine Roeckel-Drevet, Patricia |
author_facet |
Lopez, David Franchel, Jérôme Venisse, Jean-Stéphane Drevet, Patricia Label, Philippe Coutand, Catherine Roeckel-Drevet, Patricia |
author_sort |
Lopez, David |
title |
Early transcriptional response to gravistimulation in poplar without phototropic confounding factors |
title_short |
Early transcriptional response to gravistimulation in poplar without phototropic confounding factors |
title_full |
Early transcriptional response to gravistimulation in poplar without phototropic confounding factors |
title_fullStr |
Early transcriptional response to gravistimulation in poplar without phototropic confounding factors |
title_full_unstemmed |
Early transcriptional response to gravistimulation in poplar without phototropic confounding factors |
title_sort |
early transcriptional response to gravistimulation in poplar without phototropic confounding factors |
url |
http://agritrop.cirad.fr/597754/ http://agritrop.cirad.fr/597754/1/plaa071.pdf |
work_keys_str_mv |
AT lopezdavid earlytranscriptionalresponsetogravistimulationinpoplarwithoutphototropicconfoundingfactors AT francheljerome earlytranscriptionalresponsetogravistimulationinpoplarwithoutphototropicconfoundingfactors AT venissejeanstephane earlytranscriptionalresponsetogravistimulationinpoplarwithoutphototropicconfoundingfactors AT drevetpatricia earlytranscriptionalresponsetogravistimulationinpoplarwithoutphototropicconfoundingfactors AT labelphilippe earlytranscriptionalresponsetogravistimulationinpoplarwithoutphototropicconfoundingfactors AT coutandcatherine earlytranscriptionalresponsetogravistimulationinpoplarwithoutphototropicconfoundingfactors AT roeckeldrevetpatricia earlytranscriptionalresponsetogravistimulationinpoplarwithoutphototropicconfoundingfactors |
_version_ |
1792500117682520064 |