Brassinolide may control aquaporin activities in Arabidopsis thaliana
It is usually assumed that aquaporins present in the cellular membranes could be an important route in the control of water flux in plants, but evidence for this hypothesis is scarce. In this paper, we report measurements of the osmotic permeability (P os ) of protoplasts isolated from hypocotyls of wild-type and mutant Arabidopsis thaliana (L.) Heynh. Mutants were affected in their growth and exhibited different sensitivities to the phytohormone, brassinolide. For the two mutants studied (cpd: constitutive photomorphogenesis and dwarfism; bri1: brassinosteroid insensitive), hypocotyl length was correlated to P os for the protoplasts. Under experimental conditions where hypocotyl growth had ceased, restoration of root, hypocotyl and petiole growth by brassinolide was correlated with an increase in P os of the hypocotyl protoplasts. We consider that the increase in P os of the hypocotyl cells was needed because these cells were part of the transcellular water pathway of the plant. This is the first time, to our knowledge, that brassinolide has been shown to be involved in the modification of the water-transport properties of cell membranes. Our results also emphasize the importance of aquaporins and the transcellular pathway in water transport under normal growth conditions.
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Subjects: | F60 - Physiologie et biochimie végétale, Arabidopsis thaliana, brassinolide, brassinostéroïde, protoplaste, perméabilité membranaire, pression osmotique, physiologie cellulaire, physiologie végétale, http://aims.fao.org/aos/agrovoc/c_33292, http://aims.fao.org/aos/agrovoc/c_35038, http://aims.fao.org/aos/agrovoc/c_35037, http://aims.fao.org/aos/agrovoc/c_13629, http://aims.fao.org/aos/agrovoc/c_b4d8c6ad, http://aims.fao.org/aos/agrovoc/c_5443, http://aims.fao.org/aos/agrovoc/c_29151, http://aims.fao.org/aos/agrovoc/c_25189, |
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dig-cirad-fr-5975002024-01-29T03:17:52Z http://agritrop.cirad.fr/597500/ http://agritrop.cirad.fr/597500/ Brassinolide may control aquaporin activities in Arabidopsis thaliana. Morillon Raphaël, Catterou Manuella, Sangwan Rajbir S., Sangwan Brigitte S., Lassalles Jean-Paul. 2001. Planta, 212 (2) : 199-204.https://doi.org/10.1007/s004250000379 <https://doi.org/10.1007/s004250000379> Brassinolide may control aquaporin activities in Arabidopsis thaliana Morillon, Raphaël Catterou, Manuella Sangwan, Rajbir S. Sangwan, Brigitte S. Lassalles, Jean-Paul eng 2001 Planta F60 - Physiologie et biochimie végétale Arabidopsis thaliana brassinolide brassinostéroïde protoplaste perméabilité membranaire pression osmotique physiologie cellulaire physiologie végétale http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_35038 http://aims.fao.org/aos/agrovoc/c_35037 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_b4d8c6ad http://aims.fao.org/aos/agrovoc/c_5443 http://aims.fao.org/aos/agrovoc/c_29151 http://aims.fao.org/aos/agrovoc/c_25189 It is usually assumed that aquaporins present in the cellular membranes could be an important route in the control of water flux in plants, but evidence for this hypothesis is scarce. In this paper, we report measurements of the osmotic permeability (P os ) of protoplasts isolated from hypocotyls of wild-type and mutant Arabidopsis thaliana (L.) Heynh. Mutants were affected in their growth and exhibited different sensitivities to the phytohormone, brassinolide. For the two mutants studied (cpd: constitutive photomorphogenesis and dwarfism; bri1: brassinosteroid insensitive), hypocotyl length was correlated to P os for the protoplasts. Under experimental conditions where hypocotyl growth had ceased, restoration of root, hypocotyl and petiole growth by brassinolide was correlated with an increase in P os of the hypocotyl protoplasts. We consider that the increase in P os of the hypocotyl cells was needed because these cells were part of the transcellular water pathway of the plant. This is the first time, to our knowledge, that brassinolide has been shown to be involved in the modification of the water-transport properties of cell membranes. Our results also emphasize the importance of aquaporins and the transcellular pathway in water transport under normal growth conditions. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/597500/1/Morillon%20et%20al%20b%202001.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s004250000379 10.1007/s004250000379 info:eu-repo/semantics/altIdentifier/doi/10.1007/s004250000379 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s004250000379 |
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F60 - Physiologie et biochimie végétale Arabidopsis thaliana brassinolide brassinostéroïde protoplaste perméabilité membranaire pression osmotique physiologie cellulaire physiologie végétale http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_35038 http://aims.fao.org/aos/agrovoc/c_35037 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_b4d8c6ad http://aims.fao.org/aos/agrovoc/c_5443 http://aims.fao.org/aos/agrovoc/c_29151 http://aims.fao.org/aos/agrovoc/c_25189 F60 - Physiologie et biochimie végétale Arabidopsis thaliana brassinolide brassinostéroïde protoplaste perméabilité membranaire pression osmotique physiologie cellulaire physiologie végétale http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_35038 http://aims.fao.org/aos/agrovoc/c_35037 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_b4d8c6ad http://aims.fao.org/aos/agrovoc/c_5443 http://aims.fao.org/aos/agrovoc/c_29151 http://aims.fao.org/aos/agrovoc/c_25189 |
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F60 - Physiologie et biochimie végétale Arabidopsis thaliana brassinolide brassinostéroïde protoplaste perméabilité membranaire pression osmotique physiologie cellulaire physiologie végétale http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_35038 http://aims.fao.org/aos/agrovoc/c_35037 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_b4d8c6ad http://aims.fao.org/aos/agrovoc/c_5443 http://aims.fao.org/aos/agrovoc/c_29151 http://aims.fao.org/aos/agrovoc/c_25189 F60 - Physiologie et biochimie végétale Arabidopsis thaliana brassinolide brassinostéroïde protoplaste perméabilité membranaire pression osmotique physiologie cellulaire physiologie végétale http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_35038 http://aims.fao.org/aos/agrovoc/c_35037 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_b4d8c6ad http://aims.fao.org/aos/agrovoc/c_5443 http://aims.fao.org/aos/agrovoc/c_29151 http://aims.fao.org/aos/agrovoc/c_25189 Morillon, Raphaël Catterou, Manuella Sangwan, Rajbir S. Sangwan, Brigitte S. Lassalles, Jean-Paul Brassinolide may control aquaporin activities in Arabidopsis thaliana |
description |
It is usually assumed that aquaporins present in the cellular membranes could be an important route in the control of water flux in plants, but evidence for this hypothesis is scarce. In this paper, we report measurements of the osmotic permeability (P os ) of protoplasts isolated from hypocotyls of wild-type and mutant Arabidopsis thaliana (L.) Heynh. Mutants were affected in their growth and exhibited different sensitivities to the phytohormone, brassinolide. For the two mutants studied (cpd: constitutive photomorphogenesis and dwarfism; bri1: brassinosteroid insensitive), hypocotyl length was correlated to P os for the protoplasts. Under experimental conditions where hypocotyl growth had ceased, restoration of root, hypocotyl and petiole growth by brassinolide was correlated with an increase in P os of the hypocotyl protoplasts. We consider that the increase in P os of the hypocotyl cells was needed because these cells were part of the transcellular water pathway of the plant. This is the first time, to our knowledge, that brassinolide has been shown to be involved in the modification of the water-transport properties of cell membranes. Our results also emphasize the importance of aquaporins and the transcellular pathway in water transport under normal growth conditions. |
format |
article |
topic_facet |
F60 - Physiologie et biochimie végétale Arabidopsis thaliana brassinolide brassinostéroïde protoplaste perméabilité membranaire pression osmotique physiologie cellulaire physiologie végétale http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_35038 http://aims.fao.org/aos/agrovoc/c_35037 http://aims.fao.org/aos/agrovoc/c_13629 http://aims.fao.org/aos/agrovoc/c_b4d8c6ad http://aims.fao.org/aos/agrovoc/c_5443 http://aims.fao.org/aos/agrovoc/c_29151 http://aims.fao.org/aos/agrovoc/c_25189 |
author |
Morillon, Raphaël Catterou, Manuella Sangwan, Rajbir S. Sangwan, Brigitte S. Lassalles, Jean-Paul |
author_facet |
Morillon, Raphaël Catterou, Manuella Sangwan, Rajbir S. Sangwan, Brigitte S. Lassalles, Jean-Paul |
author_sort |
Morillon, Raphaël |
title |
Brassinolide may control aquaporin activities in Arabidopsis thaliana |
title_short |
Brassinolide may control aquaporin activities in Arabidopsis thaliana |
title_full |
Brassinolide may control aquaporin activities in Arabidopsis thaliana |
title_fullStr |
Brassinolide may control aquaporin activities in Arabidopsis thaliana |
title_full_unstemmed |
Brassinolide may control aquaporin activities in Arabidopsis thaliana |
title_sort |
brassinolide may control aquaporin activities in arabidopsis thaliana |
url |
http://agritrop.cirad.fr/597500/ http://agritrop.cirad.fr/597500/1/Morillon%20et%20al%20b%202001.pdf |
work_keys_str_mv |
AT morillonraphael brassinolidemaycontrolaquaporinactivitiesinarabidopsisthaliana AT catteroumanuella brassinolidemaycontrolaquaporinactivitiesinarabidopsisthaliana AT sangwanrajbirs brassinolidemaycontrolaquaporinactivitiesinarabidopsisthaliana AT sangwanbrigittes brassinolidemaycontrolaquaporinactivitiesinarabidopsisthaliana AT lassallesjeanpaul brassinolidemaycontrolaquaporinactivitiesinarabidopsisthaliana |
_version_ |
1792500095004966912 |