An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine
In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis ofPtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae)fps1 − mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin.
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Subjects: | F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, Pinus taeda, Embryogénèse somatique, expression des gènes, Transcription inverse, Xenopus laevis, Saccharomyces cerevisiae, http://aims.fao.org/aos/agrovoc/c_5913, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_35129, http://aims.fao.org/aos/agrovoc/c_49063, http://aims.fao.org/aos/agrovoc/c_6724, |
Online Access: | http://agritrop.cirad.fr/597495/ http://agritrop.cirad.fr/597495/1/Ciavatta%20et%20al%2C%202001.pdf |
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dig-cirad-fr-5974952024-01-29T03:17:45Z http://agritrop.cirad.fr/597495/ http://agritrop.cirad.fr/597495/ An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine. Ciavatta Vincent T., Morillon Raphaël, Pullman Gerald S., Chrispeels Maarten J., Cairney John. 2001. Plant Physiology, 127 : 1556-1567.https://doi.org/10.1104/pp.010793 <https://doi.org/10.1104/pp.010793> An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine Ciavatta, Vincent T. Morillon, Raphaël Pullman, Gerald S. Chrispeels, Maarten J. Cairney, John eng 2001 Plant Physiology F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Pinus taeda Embryogénèse somatique expression des gènes Transcription inverse Xenopus laevis Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_5913 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_49063 http://aims.fao.org/aos/agrovoc/c_6724 In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis ofPtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae)fps1 − mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/597495/1/Ciavatta%20et%20al%2C%202001.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1104/pp.010793 10.1104/pp.010793 info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.010793 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1104/pp.010793 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Pinus taeda Embryogénèse somatique expression des gènes Transcription inverse Xenopus laevis Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_5913 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_49063 http://aims.fao.org/aos/agrovoc/c_6724 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Pinus taeda Embryogénèse somatique expression des gènes Transcription inverse Xenopus laevis Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_5913 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_49063 http://aims.fao.org/aos/agrovoc/c_6724 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Pinus taeda Embryogénèse somatique expression des gènes Transcription inverse Xenopus laevis Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_5913 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_49063 http://aims.fao.org/aos/agrovoc/c_6724 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Pinus taeda Embryogénèse somatique expression des gènes Transcription inverse Xenopus laevis Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_5913 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_49063 http://aims.fao.org/aos/agrovoc/c_6724 Ciavatta, Vincent T. Morillon, Raphaël Pullman, Gerald S. Chrispeels, Maarten J. Cairney, John An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine |
description |
In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis ofPtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae)fps1 − mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Pinus taeda Embryogénèse somatique expression des gènes Transcription inverse Xenopus laevis Saccharomyces cerevisiae http://aims.fao.org/aos/agrovoc/c_5913 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_49063 http://aims.fao.org/aos/agrovoc/c_6724 |
author |
Ciavatta, Vincent T. Morillon, Raphaël Pullman, Gerald S. Chrispeels, Maarten J. Cairney, John |
author_facet |
Ciavatta, Vincent T. Morillon, Raphaël Pullman, Gerald S. Chrispeels, Maarten J. Cairney, John |
author_sort |
Ciavatta, Vincent T. |
title |
An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine |
title_short |
An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine |
title_full |
An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine |
title_fullStr |
An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine |
title_full_unstemmed |
An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine |
title_sort |
aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine |
url |
http://agritrop.cirad.fr/597495/ http://agritrop.cirad.fr/597495/1/Ciavatta%20et%20al%2C%202001.pdf |
work_keys_str_mv |
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1792500094400987136 |