Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange
Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.
Main Authors: | , , , , , , , , |
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Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, Dioscorea, diagnostic de laboratoire, virus des végétaux, collection botanique, conservation du matériel génétique, procédures opérationnelles normalisées d'assainissement, PCR, http://aims.fao.org/aos/agrovoc/c_2288, http://aims.fao.org/aos/agrovoc/c_16438, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_5957, http://aims.fao.org/aos/agrovoc/c_3250, http://aims.fao.org/aos/agrovoc/c_ae141fe0, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_3406, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/596984/ http://agritrop.cirad.fr/596984/1/viruses-12-01101-v2.pdf |
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dig-cirad-fr-5969842024-01-29T03:10:21Z http://agritrop.cirad.fr/596984/ http://agritrop.cirad.fr/596984/ Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange. Umber Marie, Filloux Denis, Gélabale Suzia, Gomez Rose-Marie, Marais Armelle, Gallet Séverine, Gamiette Franciane, Pavis Claudie, Teycheney Pierre-Yves. 2020. Viruses, 12 (10):1101, 18 p.https://doi.org/10.3390/v12101101 <https://doi.org/10.3390/v12101101> Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange Umber, Marie Filloux, Denis Gélabale, Suzia Gomez, Rose-Marie Marais, Armelle Gallet, Séverine Gamiette, Franciane Pavis, Claudie Teycheney, Pierre-Yves eng 2020 Viruses H20 - Maladies des plantes Dioscorea diagnostic de laboratoire virus des végétaux collection botanique conservation du matériel génétique procédures opérationnelles normalisées d'assainissement PCR http://aims.fao.org/aos/agrovoc/c_2288 http://aims.fao.org/aos/agrovoc/c_16438 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_5957 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_ae141fe0 http://aims.fao.org/aos/agrovoc/c_34079 Guadeloupe France http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/596984/1/viruses-12-01101-v2.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3390/v12101101 10.3390/v12101101 info:eu-repo/semantics/altIdentifier/doi/10.3390/v12101101 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3390/v12101101 |
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H20 - Maladies des plantes Dioscorea diagnostic de laboratoire virus des végétaux collection botanique conservation du matériel génétique procédures opérationnelles normalisées d'assainissement PCR http://aims.fao.org/aos/agrovoc/c_2288 http://aims.fao.org/aos/agrovoc/c_16438 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_5957 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_ae141fe0 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Dioscorea diagnostic de laboratoire virus des végétaux collection botanique conservation du matériel génétique procédures opérationnelles normalisées d'assainissement PCR http://aims.fao.org/aos/agrovoc/c_2288 http://aims.fao.org/aos/agrovoc/c_16438 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_5957 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_ae141fe0 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 |
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H20 - Maladies des plantes Dioscorea diagnostic de laboratoire virus des végétaux collection botanique conservation du matériel génétique procédures opérationnelles normalisées d'assainissement PCR http://aims.fao.org/aos/agrovoc/c_2288 http://aims.fao.org/aos/agrovoc/c_16438 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_5957 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_ae141fe0 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Dioscorea diagnostic de laboratoire virus des végétaux collection botanique conservation du matériel génétique procédures opérationnelles normalisées d'assainissement PCR http://aims.fao.org/aos/agrovoc/c_2288 http://aims.fao.org/aos/agrovoc/c_16438 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_5957 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_ae141fe0 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 Umber, Marie Filloux, Denis Gélabale, Suzia Gomez, Rose-Marie Marais, Armelle Gallet, Séverine Gamiette, Franciane Pavis, Claudie Teycheney, Pierre-Yves Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange |
description |
Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide. |
format |
article |
topic_facet |
H20 - Maladies des plantes Dioscorea diagnostic de laboratoire virus des végétaux collection botanique conservation du matériel génétique procédures opérationnelles normalisées d'assainissement PCR http://aims.fao.org/aos/agrovoc/c_2288 http://aims.fao.org/aos/agrovoc/c_16438 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_5957 http://aims.fao.org/aos/agrovoc/c_3250 http://aims.fao.org/aos/agrovoc/c_ae141fe0 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3406 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Umber, Marie Filloux, Denis Gélabale, Suzia Gomez, Rose-Marie Marais, Armelle Gallet, Séverine Gamiette, Franciane Pavis, Claudie Teycheney, Pierre-Yves |
author_facet |
Umber, Marie Filloux, Denis Gélabale, Suzia Gomez, Rose-Marie Marais, Armelle Gallet, Séverine Gamiette, Franciane Pavis, Claudie Teycheney, Pierre-Yves |
author_sort |
Umber, Marie |
title |
Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange |
title_short |
Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange |
title_full |
Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange |
title_fullStr |
Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange |
title_full_unstemmed |
Molecular viral diagnosis and sanitation of yam genetic resources: Implications for safe yam germplasm exchange |
title_sort |
molecular viral diagnosis and sanitation of yam genetic resources: implications for safe yam germplasm exchange |
url |
http://agritrop.cirad.fr/596984/ http://agritrop.cirad.fr/596984/1/viruses-12-01101-v2.pdf |
work_keys_str_mv |
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