Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus
Sugarcane yellow leaf virus (SCYLV) is an aphid-transmitted virus for which Melanaphis sacchari is the main vector. Almost all sugarcane varieties grown in Florida are susceptible to SCYLV infection. In this study, we investigated the prevalence of SCYLV in accessions of Sorghum bicolor which is another natural host of this virus. Two field experiments, one in 2016 with 19 sorghum lines and the other in 2017 with 15 lines, were established at Belle Glade, FL. Stalks collected randomly in planted and ratoon crops were tested by tissue-blot immunoassay (TBIA) and reverse transcription-polymerase chain reaction (RT-PCR). Over the two-year period, 366 of 423 S. bicolor samples tested positive by TBIA but SCYLV was detected by RT-PCR in only 12 of 161 randomly selected subsamples. Full genome sequences of SCYLV, sugarcane mosaic virus (SCMV) and a new marafivirus were obtained by high-throughput sequencing (HTS) from three TBIA positive sorghum samples. HTS data for all three viruses were confirmed by RT-PCR. The SCMV isolates from S. bicolor appeared to be a new strain of this virus species. Positive reaction of S. bicolor by TBIA using SCYLV antibodies could not be systematically associated with plant infection by SCYLV or another virus. This suggested the occurrence of a non-specific serological reaction with an unknown S. bicolor antigen. SCMV and the new marafivirus were also detected in Sorghum almum, suggesting that this weed is a reservoir for S. bicolor-infecting viruses in Florida.
Main Authors: | , , , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, Sorghum, Marafivirus, virus mosaïque canne à sucre, séquence d'adn, PCR, http://aims.fao.org/aos/agrovoc/c_7244, http://aims.fao.org/aos/agrovoc/c_413fad7e, http://aims.fao.org/aos/agrovoc/c_35631, http://aims.fao.org/aos/agrovoc/c_27812, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_2985, http://aims.fao.org/aos/agrovoc/c_8114, |
Online Access: | http://agritrop.cirad.fr/596536/ http://agritrop.cirad.fr/596536/7/596536_MAA.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-596536 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5965362024-01-29T03:02:34Z http://agritrop.cirad.fr/596536/ http://agritrop.cirad.fr/596536/ Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus. Boukari Wardatou, Mollov Dimitre S., Wei Chunyan, Tang Lihua, Grinstead Samuel, Nouman Tahir Muhammad, Mulandesa Eva, Hincapie Martha, Beiriger Robert, Rott Philippe. 2021. Crop Protection, 139:105373, 9 p.https://doi.org/10.1016/j.cropro.2020.105373 <https://doi.org/10.1016/j.cropro.2020.105373> Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus Boukari, Wardatou Mollov, Dimitre S. Wei, Chunyan Tang, Lihua Grinstead, Samuel Nouman Tahir, Muhammad Mulandesa, Eva Hincapie, Martha Beiriger, Robert Rott, Philippe eng 2021 Crop Protection H20 - Maladies des plantes Sorghum Marafivirus virus mosaïque canne à sucre séquence d'adn PCR http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_413fad7e http://aims.fao.org/aos/agrovoc/c_35631 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_34079 Floride États-Unis d'Amérique http://aims.fao.org/aos/agrovoc/c_2985 http://aims.fao.org/aos/agrovoc/c_8114 Sugarcane yellow leaf virus (SCYLV) is an aphid-transmitted virus for which Melanaphis sacchari is the main vector. Almost all sugarcane varieties grown in Florida are susceptible to SCYLV infection. In this study, we investigated the prevalence of SCYLV in accessions of Sorghum bicolor which is another natural host of this virus. Two field experiments, one in 2016 with 19 sorghum lines and the other in 2017 with 15 lines, were established at Belle Glade, FL. Stalks collected randomly in planted and ratoon crops were tested by tissue-blot immunoassay (TBIA) and reverse transcription-polymerase chain reaction (RT-PCR). Over the two-year period, 366 of 423 S. bicolor samples tested positive by TBIA but SCYLV was detected by RT-PCR in only 12 of 161 randomly selected subsamples. Full genome sequences of SCYLV, sugarcane mosaic virus (SCMV) and a new marafivirus were obtained by high-throughput sequencing (HTS) from three TBIA positive sorghum samples. HTS data for all three viruses were confirmed by RT-PCR. The SCMV isolates from S. bicolor appeared to be a new strain of this virus species. Positive reaction of S. bicolor by TBIA using SCYLV antibodies could not be systematically associated with plant infection by SCYLV or another virus. This suggested the occurrence of a non-specific serological reaction with an unknown S. bicolor antigen. SCMV and the new marafivirus were also detected in Sorghum almum, suggesting that this weed is a reservoir for S. bicolor-infecting viruses in Florida. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/acceptedVersion http://agritrop.cirad.fr/596536/7/596536_MAA.pdf text cc_by_nc info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/4.0/ https://doi.org/10.1016/j.cropro.2020.105373 10.1016/j.cropro.2020.105373 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2020.105373 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.cropro.2020.105373 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes Sorghum Marafivirus virus mosaïque canne à sucre séquence d'adn PCR http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_413fad7e http://aims.fao.org/aos/agrovoc/c_35631 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2985 http://aims.fao.org/aos/agrovoc/c_8114 H20 - Maladies des plantes Sorghum Marafivirus virus mosaïque canne à sucre séquence d'adn PCR http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_413fad7e http://aims.fao.org/aos/agrovoc/c_35631 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2985 http://aims.fao.org/aos/agrovoc/c_8114 |
spellingShingle |
H20 - Maladies des plantes Sorghum Marafivirus virus mosaïque canne à sucre séquence d'adn PCR http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_413fad7e http://aims.fao.org/aos/agrovoc/c_35631 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2985 http://aims.fao.org/aos/agrovoc/c_8114 H20 - Maladies des plantes Sorghum Marafivirus virus mosaïque canne à sucre séquence d'adn PCR http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_413fad7e http://aims.fao.org/aos/agrovoc/c_35631 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2985 http://aims.fao.org/aos/agrovoc/c_8114 Boukari, Wardatou Mollov, Dimitre S. Wei, Chunyan Tang, Lihua Grinstead, Samuel Nouman Tahir, Muhammad Mulandesa, Eva Hincapie, Martha Beiriger, Robert Rott, Philippe Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus |
description |
Sugarcane yellow leaf virus (SCYLV) is an aphid-transmitted virus for which Melanaphis sacchari is the main vector. Almost all sugarcane varieties grown in Florida are susceptible to SCYLV infection. In this study, we investigated the prevalence of SCYLV in accessions of Sorghum bicolor which is another natural host of this virus. Two field experiments, one in 2016 with 19 sorghum lines and the other in 2017 with 15 lines, were established at Belle Glade, FL. Stalks collected randomly in planted and ratoon crops were tested by tissue-blot immunoassay (TBIA) and reverse transcription-polymerase chain reaction (RT-PCR). Over the two-year period, 366 of 423 S. bicolor samples tested positive by TBIA but SCYLV was detected by RT-PCR in only 12 of 161 randomly selected subsamples. Full genome sequences of SCYLV, sugarcane mosaic virus (SCMV) and a new marafivirus were obtained by high-throughput sequencing (HTS) from three TBIA positive sorghum samples. HTS data for all three viruses were confirmed by RT-PCR. The SCMV isolates from S. bicolor appeared to be a new strain of this virus species. Positive reaction of S. bicolor by TBIA using SCYLV antibodies could not be systematically associated with plant infection by SCYLV or another virus. This suggested the occurrence of a non-specific serological reaction with an unknown S. bicolor antigen. SCMV and the new marafivirus were also detected in Sorghum almum, suggesting that this weed is a reservoir for S. bicolor-infecting viruses in Florida. |
format |
article |
topic_facet |
H20 - Maladies des plantes Sorghum Marafivirus virus mosaïque canne à sucre séquence d'adn PCR http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_413fad7e http://aims.fao.org/aos/agrovoc/c_35631 http://aims.fao.org/aos/agrovoc/c_27812 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_2985 http://aims.fao.org/aos/agrovoc/c_8114 |
author |
Boukari, Wardatou Mollov, Dimitre S. Wei, Chunyan Tang, Lihua Grinstead, Samuel Nouman Tahir, Muhammad Mulandesa, Eva Hincapie, Martha Beiriger, Robert Rott, Philippe |
author_facet |
Boukari, Wardatou Mollov, Dimitre S. Wei, Chunyan Tang, Lihua Grinstead, Samuel Nouman Tahir, Muhammad Mulandesa, Eva Hincapie, Martha Beiriger, Robert Rott, Philippe |
author_sort |
Boukari, Wardatou |
title |
Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus |
title_short |
Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus |
title_full |
Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus |
title_fullStr |
Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus |
title_full_unstemmed |
Screening for sugarcane yellow leaf virus in sorghum in Florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus |
title_sort |
screening for sugarcane yellow leaf virus in sorghum in florida revealed its occurrence in mixed infections with sugarcane mosaic virus and a new marafivirus |
url |
http://agritrop.cirad.fr/596536/ http://agritrop.cirad.fr/596536/7/596536_MAA.pdf |
work_keys_str_mv |
AT boukariwardatou screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT mollovdimitres screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT weichunyan screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT tanglihua screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT grinsteadsamuel screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT noumantahirmuhammad screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT mulandesaeva screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT hincapiemartha screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT beirigerrobert screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus AT rottphilippe screeningforsugarcaneyellowleafvirusinsorghuminfloridarevealeditsoccurrenceinmixedinfectionswithsugarcanemosaicvirusandanewmarafivirus |
_version_ |
1792500023333748736 |