Identification of a murine cell line that distinguishes virulent from attenuated isolates of the morbillivirus Peste des Petits Ruminants, a promising tool for virulence studies
Comprehensive pathogenesis studies on Peste des Petits Ruminants virus (PPRV) have been delayed so far by the absence of a small animal model reproducing the disease or an in vitro biological system revealing virulence differences. In this study, a mouse 10T1/2 cell line has been identified as presenting different susceptibility to virulent and attenuated PPRV strains. As evidenced by immunofluorescence test and RT-PCR, both virulent and attenuated PPR viruses penetrated and initiated the replication cycle in 10T1/2 cells, independently of the presence of the SLAM goat receptor. However, only virulent strains successfully completed their replication cycle while the vaccine strains did not. Since 10T1/2 cells are interferon-producing cells, the role of the type I interferon (type I IFN) response on this differentiated replication between virulent and attenuated strains was verified by stimulation or repression. Modulation of the type I IFN response did not improve the replication of the vaccine strains, indicating that other cell factor(s) not yet established may hinder the replication of attenuated PPRV in 10T1/2. This 10T1/2 cell line can be proposed as a new in vitro tool for PPRV-host interaction and virulence studies.
| Main Authors: | , , , , , , , |
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| Format: | article biblioteca |
| Language: | eng |
| Subjects: | L73 - Maladies des animaux, virus peste petits ruminants, relation hôte pathogène, morbillivirus, virulence, virus pathogène, pathogénèse, peste des petits ruminants, Murinae, http://aims.fao.org/aos/agrovoc/c_16581, http://aims.fao.org/aos/agrovoc/c_34017, http://aims.fao.org/aos/agrovoc/c_4937, http://aims.fao.org/aos/agrovoc/c_8261, http://aims.fao.org/aos/agrovoc/c_34318, http://aims.fao.org/aos/agrovoc/c_24881, http://aims.fao.org/aos/agrovoc/c_16789, http://aims.fao.org/aos/agrovoc/c_7abe89f8, |
| Online Access: | http://agritrop.cirad.fr/595977/ http://agritrop.cirad.fr/595977/1/Comerlato%20et%20al%202020.pdf |
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