Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans
Sugarcane (Saccharum spp. hybrids) is a major source of sugar and renewable bioenergy crop worldwide and suffers serious yield losses due to many pathogen infections. Leaf scald caused by Xanthomonas albilineans is a major bacterial disease of sugarcane in most sugarcane-planting countries. The molecular mechanisms of resistance to leaf scald in this plant are, however, still unclear. We performed a comparative transcriptome analysis between resistant (LCP 85-384) and susceptible (ROC20) sugarcane cultivars infected by X. albilineans using the RNA-seq platform. 24 cDNA libraries were generated with RNA isolated at four time points (0, 24, 48, and 72 h post inoculation) from the two cultivars with three biological replicates. A total of 105,783 differentially expressed genes (DEGs) were identified in both cultivars and the most upregulated and downregulated DEGs were annotated for the processes of the metabolic and single-organism categories, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the 7612 DEGs showed that plant–pathogen interaction, spliceosome, glutathione metabolism, protein processing in endoplasmic reticulum, and plant hormone signal transduction contributed to sugarcane's response to X. albilineans infection. Subsequently, relative expression levels of ten DEGs determined by quantitative reverse transcription-PCR (qRT-PCR), in addition to RNA-Seq data, indicated that different plant hormone (auxin and ethylene) signal transduction pathways play essential roles in sugarcane infected by X. albilineans. In conclusion, our results provide, for the first time, valuable information regarding the transcriptome changes in sugarcane in response to infection by X. albilineans, which contribute to the understanding of the molecular mechanisms underlying the interactions between sugarcane and this pathogen and provide important clues for further characterization of leaf scald resistance in sugarcane.
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Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Saccharum, canne à sucre, Xanthomonas albilineans, expression des gènes, résistance aux maladies, relation hôte pathogène, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_7501, http://aims.fao.org/aos/agrovoc/c_27422, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_34017, |
Online Access: | http://agritrop.cirad.fr/594405/ http://agritrop.cirad.fr/594405/1/Transcriptome%20SC%20leaf%20scald_2019%20ijms-20-06138.pdf |
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dig-cirad-fr-5944052024-01-29T02:27:33Z http://agritrop.cirad.fr/594405/ http://agritrop.cirad.fr/594405/ Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans. Ntambo Mbuya Sylvain, Meng Jian-Yu, Rott Philippe, Henry Robert, Zhang Hui-Li, Gao San-Ji. 2019. International Journal of Molecular Sciences, 20 (24):6138, 20 p.https://doi.org/10.3390/ijms20246138 <https://doi.org/10.3390/ijms20246138> Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans Ntambo, Mbuya Sylvain Meng, Jian-Yu Rott, Philippe Henry, Robert Zhang, Hui-Li Gao, San-Ji eng 2019 International Journal of Molecular Sciences H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Saccharum canne à sucre Xanthomonas albilineans expression des gènes résistance aux maladies relation hôte pathogène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_7501 http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_34017 Sugarcane (Saccharum spp. hybrids) is a major source of sugar and renewable bioenergy crop worldwide and suffers serious yield losses due to many pathogen infections. Leaf scald caused by Xanthomonas albilineans is a major bacterial disease of sugarcane in most sugarcane-planting countries. The molecular mechanisms of resistance to leaf scald in this plant are, however, still unclear. We performed a comparative transcriptome analysis between resistant (LCP 85-384) and susceptible (ROC20) sugarcane cultivars infected by X. albilineans using the RNA-seq platform. 24 cDNA libraries were generated with RNA isolated at four time points (0, 24, 48, and 72 h post inoculation) from the two cultivars with three biological replicates. A total of 105,783 differentially expressed genes (DEGs) were identified in both cultivars and the most upregulated and downregulated DEGs were annotated for the processes of the metabolic and single-organism categories, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the 7612 DEGs showed that plant–pathogen interaction, spliceosome, glutathione metabolism, protein processing in endoplasmic reticulum, and plant hormone signal transduction contributed to sugarcane's response to X. albilineans infection. Subsequently, relative expression levels of ten DEGs determined by quantitative reverse transcription-PCR (qRT-PCR), in addition to RNA-Seq data, indicated that different plant hormone (auxin and ethylene) signal transduction pathways play essential roles in sugarcane infected by X. albilineans. In conclusion, our results provide, for the first time, valuable information regarding the transcriptome changes in sugarcane in response to infection by X. albilineans, which contribute to the understanding of the molecular mechanisms underlying the interactions between sugarcane and this pathogen and provide important clues for further characterization of leaf scald resistance in sugarcane. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/594405/1/Transcriptome%20SC%20leaf%20scald_2019%20ijms-20-06138.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3390/ijms20246138 10.3390/ijms20246138 info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20246138 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3390/ijms20246138 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Saccharum canne à sucre Xanthomonas albilineans expression des gènes résistance aux maladies relation hôte pathogène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_7501 http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_34017 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Saccharum canne à sucre Xanthomonas albilineans expression des gènes résistance aux maladies relation hôte pathogène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_7501 http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_34017 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Saccharum canne à sucre Xanthomonas albilineans expression des gènes résistance aux maladies relation hôte pathogène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_7501 http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_34017 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Saccharum canne à sucre Xanthomonas albilineans expression des gènes résistance aux maladies relation hôte pathogène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_7501 http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_34017 Ntambo, Mbuya Sylvain Meng, Jian-Yu Rott, Philippe Henry, Robert Zhang, Hui-Li Gao, San-Ji Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans |
description |
Sugarcane (Saccharum spp. hybrids) is a major source of sugar and renewable bioenergy crop worldwide and suffers serious yield losses due to many pathogen infections. Leaf scald caused by Xanthomonas albilineans is a major bacterial disease of sugarcane in most sugarcane-planting countries. The molecular mechanisms of resistance to leaf scald in this plant are, however, still unclear. We performed a comparative transcriptome analysis between resistant (LCP 85-384) and susceptible (ROC20) sugarcane cultivars infected by X. albilineans using the RNA-seq platform. 24 cDNA libraries were generated with RNA isolated at four time points (0, 24, 48, and 72 h post inoculation) from the two cultivars with three biological replicates. A total of 105,783 differentially expressed genes (DEGs) were identified in both cultivars and the most upregulated and downregulated DEGs were annotated for the processes of the metabolic and single-organism categories, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the 7612 DEGs showed that plant–pathogen interaction, spliceosome, glutathione metabolism, protein processing in endoplasmic reticulum, and plant hormone signal transduction contributed to sugarcane's response to X. albilineans infection. Subsequently, relative expression levels of ten DEGs determined by quantitative reverse transcription-PCR (qRT-PCR), in addition to RNA-Seq data, indicated that different plant hormone (auxin and ethylene) signal transduction pathways play essential roles in sugarcane infected by X. albilineans. In conclusion, our results provide, for the first time, valuable information regarding the transcriptome changes in sugarcane in response to infection by X. albilineans, which contribute to the understanding of the molecular mechanisms underlying the interactions between sugarcane and this pathogen and provide important clues for further characterization of leaf scald resistance in sugarcane. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Saccharum canne à sucre Xanthomonas albilineans expression des gènes résistance aux maladies relation hôte pathogène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_7501 http://aims.fao.org/aos/agrovoc/c_27422 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_34017 |
author |
Ntambo, Mbuya Sylvain Meng, Jian-Yu Rott, Philippe Henry, Robert Zhang, Hui-Li Gao, San-Ji |
author_facet |
Ntambo, Mbuya Sylvain Meng, Jian-Yu Rott, Philippe Henry, Robert Zhang, Hui-Li Gao, San-Ji |
author_sort |
Ntambo, Mbuya Sylvain |
title |
Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans |
title_short |
Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans |
title_full |
Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans |
title_fullStr |
Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans |
title_full_unstemmed |
Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans |
title_sort |
comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by xanthomonas albilineans |
url |
http://agritrop.cirad.fr/594405/ http://agritrop.cirad.fr/594405/1/Transcriptome%20SC%20leaf%20scald_2019%20ijms-20-06138.pdf |
work_keys_str_mv |
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