Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches

Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches

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Visacane, CIRAD's sugarcane quarantine program (http://visacane.cirad.fr/en/) covers three main quarantine procedures: detection of pests and pathogens, elimination of pests and pathogens, and transfer of pest and pathogen-free planting material. While we aim at improving sugarcane adaptability, stability and sustainability through Visacane, sugarcane clones and related wild species with high levels of genetic diversity and originating from multiple sources are quarantined at Montpellier (France) to be sent to Visacane's partners. From 2013 to 2017, 2,830 batches of disease-free cuttings from 435 sugarcane clones were transferred to 42 partners of Visacane. Our main goal is to foster worldwide distribution of pest- and pathogen-free planting material and to guarantee breeder's rights. The virome of sugarcane was recently investigated using viral metagenomics and the genetic diversity of three uncharacterized sugarcane viruses (sugarcane white streak virus, sugarcane striate virus and sugarcane mild mosaic virus) was assessed for improved diagnosis. Hence, new protocols to detect these three viruses were developed and validated. An additional detection protocol targeting sugarcane bacilliform virus, a virus that was up to now not considered as a quarantine organism by many countries and undiagnosed in quarantine, was also developed. These issues are essential to guarantee the health status of the planting material and to increase the guaranties of Visacane's sugarcane germplasm.

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Main Authors: Fernandez, Emmanuel, Ferdinand, Romain, Filloux, Denis, Guinet, Isabelle, Julian, Charlotte, Rott, Philippe, Roumagnac, Philippe, Daugrois, Jean-Heinrich
Format: conference_item biblioteca
Language:eng
Published: ISSCT
Online Access:http://agritrop.cirad.fr/593450/
http://agritrop.cirad.fr/593450/1/Fernandez%2C%20Ferdinand%2C%20Filloux%2C%20Guinet%2C%20Julian%2C%20Rott%2C%20Roumagnac%2C%20Daugrois.pdf%20XXXissct.pdf
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spelling dig-cirad-fr-5934502020-09-23T08:58:24Z http://agritrop.cirad.fr/593450/ http://agritrop.cirad.fr/593450/ Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches. Fernandez Emmanuel, Ferdinand Romain, Filloux Denis, Guinet Isabelle, Julian Charlotte, Rott Philippe, Roumagnac Philippe, Daugrois Jean-Heinrich. 2019. In : Proceedings of the XXX International Society of Sugar Cane Technologists Congress. ISSCT. Tucuman : ISSCT, 345-351. ISBN 978-616-92761-0-4 ISSCT Congress. 30, Tucuman, Argentine, 2 Septembre 2019/5 Septembre 2019. Researchers Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches Fernandez, Emmanuel Ferdinand, Romain Filloux, Denis Guinet, Isabelle Julian, Charlotte Rott, Philippe Roumagnac, Philippe Daugrois, Jean-Heinrich eng 2019 ISSCT Proceedings of the XXX International Society of Sugar Cane Technologists Congress Visacane, CIRAD's sugarcane quarantine program (http://visacane.cirad.fr/en/) covers three main quarantine procedures: detection of pests and pathogens, elimination of pests and pathogens, and transfer of pest and pathogen-free planting material. While we aim at improving sugarcane adaptability, stability and sustainability through Visacane, sugarcane clones and related wild species with high levels of genetic diversity and originating from multiple sources are quarantined at Montpellier (France) to be sent to Visacane's partners. From 2013 to 2017, 2,830 batches of disease-free cuttings from 435 sugarcane clones were transferred to 42 partners of Visacane. Our main goal is to foster worldwide distribution of pest- and pathogen-free planting material and to guarantee breeder's rights. The virome of sugarcane was recently investigated using viral metagenomics and the genetic diversity of three uncharacterized sugarcane viruses (sugarcane white streak virus, sugarcane striate virus and sugarcane mild mosaic virus) was assessed for improved diagnosis. Hence, new protocols to detect these three viruses were developed and validated. An additional detection protocol targeting sugarcane bacilliform virus, a virus that was up to now not considered as a quarantine organism by many countries and undiagnosed in quarantine, was also developed. These issues are essential to guarantee the health status of the planting material and to increase the guaranties of Visacane's sugarcane germplasm. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/593450/1/Fernandez%2C%20Ferdinand%2C%20Filloux%2C%20Guinet%2C%20Julian%2C%20Rott%2C%20Roumagnac%2C%20Daugrois.pdf%20XXXissct.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html
institution CIRAD FR
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country Francia
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databasecode dig-cirad-fr
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region Europa del Oeste
libraryname Biblioteca del CIRAD Francia
language eng
description Visacane, CIRAD's sugarcane quarantine program (http://visacane.cirad.fr/en/) covers three main quarantine procedures: detection of pests and pathogens, elimination of pests and pathogens, and transfer of pest and pathogen-free planting material. While we aim at improving sugarcane adaptability, stability and sustainability through Visacane, sugarcane clones and related wild species with high levels of genetic diversity and originating from multiple sources are quarantined at Montpellier (France) to be sent to Visacane's partners. From 2013 to 2017, 2,830 batches of disease-free cuttings from 435 sugarcane clones were transferred to 42 partners of Visacane. Our main goal is to foster worldwide distribution of pest- and pathogen-free planting material and to guarantee breeder's rights. The virome of sugarcane was recently investigated using viral metagenomics and the genetic diversity of three uncharacterized sugarcane viruses (sugarcane white streak virus, sugarcane striate virus and sugarcane mild mosaic virus) was assessed for improved diagnosis. Hence, new protocols to detect these three viruses were developed and validated. An additional detection protocol targeting sugarcane bacilliform virus, a virus that was up to now not considered as a quarantine organism by many countries and undiagnosed in quarantine, was also developed. These issues are essential to guarantee the health status of the planting material and to increase the guaranties of Visacane's sugarcane germplasm.
format conference_item
author Fernandez, Emmanuel
Ferdinand, Romain
Filloux, Denis
Guinet, Isabelle
Julian, Charlotte
Rott, Philippe
Roumagnac, Philippe
Daugrois, Jean-Heinrich
spellingShingle Fernandez, Emmanuel
Ferdinand, Romain
Filloux, Denis
Guinet, Isabelle
Julian, Charlotte
Rott, Philippe
Roumagnac, Philippe
Daugrois, Jean-Heinrich
Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches
author_facet Fernandez, Emmanuel
Ferdinand, Romain
Filloux, Denis
Guinet, Isabelle
Julian, Charlotte
Rott, Philippe
Roumagnac, Philippe
Daugrois, Jean-Heinrich
author_sort Fernandez, Emmanuel
title Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches
title_short Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches
title_full Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches
title_fullStr Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches
title_full_unstemmed Improvements in virus detection at CIRAD's sugarcane quarantine using both viral metagenomics and PCR-based approaches
title_sort improvements in virus detection at cirad's sugarcane quarantine using both viral metagenomics and pcr-based approaches
publisher ISSCT
url http://agritrop.cirad.fr/593450/
http://agritrop.cirad.fr/593450/1/Fernandez%2C%20Ferdinand%2C%20Filloux%2C%20Guinet%2C%20Julian%2C%20Rott%2C%20Roumagnac%2C%20Daugrois.pdf%20XXXissct.pdf
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AT fillouxdenis improvementsinvirusdetectionatciradssugarcanequarantineusingbothviralmetagenomicsandpcrbasedapproaches
AT guinetisabelle improvementsinvirusdetectionatciradssugarcanequarantineusingbothviralmetagenomicsandpcrbasedapproaches
AT juliancharlotte improvementsinvirusdetectionatciradssugarcanequarantineusingbothviralmetagenomicsandpcrbasedapproaches
AT rottphilippe improvementsinvirusdetectionatciradssugarcanequarantineusingbothviralmetagenomicsandpcrbasedapproaches
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AT daugroisjeanheinrich improvementsinvirusdetectionatciradssugarcanequarantineusingbothviralmetagenomicsandpcrbasedapproaches
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