Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents
Trypanosoma (Herpetosoma) lewisi is a cosmopolitan parasite of rodents strongly linked to the human dispersal of Rattus spp. from Asia to the rest of the world. This species is highly phylogenetically related to trypanosomes from other rodents (T. lewisi-like), and sporadically infects other mammals. T. lewisi may opportunistically infect humans, and has been considered an emergent rat-borne zoonosis associated to poverty. We developed the THeCATL-PCR based on Cathepsin L (CATL) sequences to specifically detect T. (Herpetosoma) spp., and assess their genetic diversity. This method exhibited high sensitivity using blood samples, and is the first molecular method employed to search for T. lewisi in its flea vectors. THeCATL-PCR surveys using simple DNA preparation from blood preserved in ethanol or filter paper detected T. lewisi in Rattus spp. from human dwellings in South America (Brazil and Venezuela), East Africa (Mozambique), and Southeast Asia (Thailand, Cambodia and Lao PDR). In addition, native rodents captured in anthropogenic and nearby human settlements in natural habitats harbored T. (Herpetosoma) spp. PCR-amplified CATL gene fragments (253 bp) distinguish T. lewisi and T. lewisi-like from other trypanosomes, and allow for assessment of genetic diversity and relationships among T. (Herpetosoma) spp. Our molecular surveys corroborated worldwide high prevalence of T. lewisi, incriminating Mastomys natalensis as an important carrier of this species in Africa, and supported its spillover from invader Rattus spp. to native rodents in Brazil and Mozambique. THeCATL-PCR provided new insights on the accurate diagnosis and genetic repertoire of T. (Herpetosoma) spp. in rodent and non-rodent hosts, revealing a novel species of this subgenus in an African gerbil. Phylogenetic analysis based on CATL sequences from T. (Herpetosoma) spp. and other trypanosomes (amplified using pan-trypanosome primers) uncovered rodents harboring, beyond mammal trypanosomes of different subgenera, some species that clustered in the lizard-snake clade of trypanosomes.
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dig-cirad-fr-591905 |
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Francia |
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Bibliográfico |
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Europa del Oeste |
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Biblioteca del CIRAD Francia |
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eng |
topic |
L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Rattus Trypanosoma lewisi trypanosomose génétique sang Siphonaptera rongeur http://aims.fao.org/aos/agrovoc/c_15663 http://aims.fao.org/aos/agrovoc/c_27405 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_7082 http://aims.fao.org/aos/agrovoc/c_13948 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_7701 http://aims.fao.org/aos/agrovoc/c_12076 http://aims.fao.org/aos/agrovoc/c_4073 http://aims.fao.org/aos/agrovoc/c_8186 L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Rattus Trypanosoma lewisi trypanosomose génétique sang Siphonaptera rongeur http://aims.fao.org/aos/agrovoc/c_15663 http://aims.fao.org/aos/agrovoc/c_27405 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_7082 http://aims.fao.org/aos/agrovoc/c_13948 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_7701 http://aims.fao.org/aos/agrovoc/c_12076 http://aims.fao.org/aos/agrovoc/c_4073 http://aims.fao.org/aos/agrovoc/c_8186 |
spellingShingle |
L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Rattus Trypanosoma lewisi trypanosomose génétique sang Siphonaptera rongeur http://aims.fao.org/aos/agrovoc/c_15663 http://aims.fao.org/aos/agrovoc/c_27405 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_7082 http://aims.fao.org/aos/agrovoc/c_13948 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_7701 http://aims.fao.org/aos/agrovoc/c_12076 http://aims.fao.org/aos/agrovoc/c_4073 http://aims.fao.org/aos/agrovoc/c_8186 L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Rattus Trypanosoma lewisi trypanosomose génétique sang Siphonaptera rongeur http://aims.fao.org/aos/agrovoc/c_15663 http://aims.fao.org/aos/agrovoc/c_27405 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_7082 http://aims.fao.org/aos/agrovoc/c_13948 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_7701 http://aims.fao.org/aos/agrovoc/c_12076 http://aims.fao.org/aos/agrovoc/c_4073 http://aims.fao.org/aos/agrovoc/c_8186 Ortiz, Paola A. Garcia, Herakles A. Lima, Luciana da Silva, Flávia Maia Campaner, Marta Pereira, Carlos L. Jittapalapong, Sathaporn Neves, Luis Desquesnes, Marc Camargo, Erney P. Teixeira, Marta M.G. Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents |
description |
Trypanosoma (Herpetosoma) lewisi is a cosmopolitan parasite of rodents strongly linked to the human dispersal of Rattus spp. from Asia to the rest of the world. This species is highly phylogenetically related to trypanosomes from other rodents (T. lewisi-like), and sporadically infects other mammals. T. lewisi may opportunistically infect humans, and has been considered an emergent rat-borne zoonosis associated to poverty. We developed the THeCATL-PCR based on Cathepsin L (CATL) sequences to specifically detect T. (Herpetosoma) spp., and assess their genetic diversity. This method exhibited high sensitivity using blood samples, and is the first molecular method employed to search for T. lewisi in its flea vectors. THeCATL-PCR surveys using simple DNA preparation from blood preserved in ethanol or filter paper detected T. lewisi in Rattus spp. from human dwellings in South America (Brazil and Venezuela), East Africa (Mozambique), and Southeast Asia (Thailand, Cambodia and Lao PDR). In addition, native rodents captured in anthropogenic and nearby human settlements in natural habitats harbored T. (Herpetosoma) spp. PCR-amplified CATL gene fragments (253 bp) distinguish T. lewisi and T. lewisi-like from other trypanosomes, and allow for assessment of genetic diversity and relationships among T. (Herpetosoma) spp. Our molecular surveys corroborated worldwide high prevalence of T. lewisi, incriminating Mastomys natalensis as an important carrier of this species in Africa, and supported its spillover from invader Rattus spp. to native rodents in Brazil and Mozambique. THeCATL-PCR provided new insights on the accurate diagnosis and genetic repertoire of T. (Herpetosoma) spp. in rodent and non-rodent hosts, revealing a novel species of this subgenus in an African gerbil. Phylogenetic analysis based on CATL sequences from T. (Herpetosoma) spp. and other trypanosomes (amplified using pan-trypanosome primers) uncovered rodents harboring, beyond mammal trypanosomes of different subgenera, some species that clustered in the lizard-snake clade of trypanosomes. |
format |
article |
topic_facet |
L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Rattus Trypanosoma lewisi trypanosomose génétique sang Siphonaptera rongeur http://aims.fao.org/aos/agrovoc/c_15663 http://aims.fao.org/aos/agrovoc/c_27405 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_7082 http://aims.fao.org/aos/agrovoc/c_13948 http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_7701 http://aims.fao.org/aos/agrovoc/c_12076 http://aims.fao.org/aos/agrovoc/c_4073 http://aims.fao.org/aos/agrovoc/c_8186 |
author |
Ortiz, Paola A. Garcia, Herakles A. Lima, Luciana da Silva, Flávia Maia Campaner, Marta Pereira, Carlos L. Jittapalapong, Sathaporn Neves, Luis Desquesnes, Marc Camargo, Erney P. Teixeira, Marta M.G. |
author_facet |
Ortiz, Paola A. Garcia, Herakles A. Lima, Luciana da Silva, Flávia Maia Campaner, Marta Pereira, Carlos L. Jittapalapong, Sathaporn Neves, Luis Desquesnes, Marc Camargo, Erney P. Teixeira, Marta M.G. |
author_sort |
Ortiz, Paola A. |
title |
Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents |
title_short |
Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents |
title_full |
Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents |
title_fullStr |
Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents |
title_full_unstemmed |
Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents |
title_sort |
diagnosis and genetic analysis of the worldwide distributed rattus-borne trypanosoma(herpetosoma)lewisi and its allied species in blood andfleas ofrodents |
url |
http://agritrop.cirad.fr/591905/ http://agritrop.cirad.fr/591905/1/Diagnosis%20and%20genetics%20analysis%20of%20the%20worldwide%20distributed%20rattus-borne%20Tryapanosoma%20lewisi%20and%20its%20allied%20species%20in%20blood%20and%20feas%20of%20rodents.pdf |
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dig-cirad-fr-5919052024-01-29T01:49:32Z http://agritrop.cirad.fr/591905/ http://agritrop.cirad.fr/591905/ Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents. Ortiz Paola A., Garcia Herakles A., Lima Luciana, da Silva Flávia Maia, Campaner Marta, Pereira Carlos L., Jittapalapong Sathaporn, Neves Luis, Desquesnes Marc, Camargo Erney P., Teixeira Marta M.G.. 2018. Infection, Genetics and Evolution, 63 : 380-390.https://doi.org/10.1016/j.meegid.2017.09.001 <https://doi.org/10.1016/j.meegid.2017.09.001> Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma(Herpetosoma)lewisi and its allied species in blood andfleas ofrodents Ortiz, Paola A. Garcia, Herakles A. Lima, Luciana da Silva, Flávia Maia Campaner, Marta Pereira, Carlos L. Jittapalapong, Sathaporn Neves, Luis Desquesnes, Marc Camargo, Erney P. Teixeira, Marta M.G. eng 2018 Infection, Genetics and Evolution L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Rattus Trypanosoma lewisi trypanosomose génétique sang Siphonaptera rongeur http://aims.fao.org/aos/agrovoc/c_15663 http://aims.fao.org/aos/agrovoc/c_27405 http://aims.fao.org/aos/agrovoc/c_7988 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_7082 http://aims.fao.org/aos/agrovoc/c_13948 Brésil Mozambique Thaïlande République démocratique populaire lao Cambodge Venezuela (République bolivarienne du) http://aims.fao.org/aos/agrovoc/c_1070 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_7701 http://aims.fao.org/aos/agrovoc/c_12076 http://aims.fao.org/aos/agrovoc/c_4073 http://aims.fao.org/aos/agrovoc/c_8186 Trypanosoma (Herpetosoma) lewisi is a cosmopolitan parasite of rodents strongly linked to the human dispersal of Rattus spp. from Asia to the rest of the world. This species is highly phylogenetically related to trypanosomes from other rodents (T. lewisi-like), and sporadically infects other mammals. T. lewisi may opportunistically infect humans, and has been considered an emergent rat-borne zoonosis associated to poverty. We developed the THeCATL-PCR based on Cathepsin L (CATL) sequences to specifically detect T. (Herpetosoma) spp., and assess their genetic diversity. This method exhibited high sensitivity using blood samples, and is the first molecular method employed to search for T. lewisi in its flea vectors. THeCATL-PCR surveys using simple DNA preparation from blood preserved in ethanol or filter paper detected T. lewisi in Rattus spp. from human dwellings in South America (Brazil and Venezuela), East Africa (Mozambique), and Southeast Asia (Thailand, Cambodia and Lao PDR). In addition, native rodents captured in anthropogenic and nearby human settlements in natural habitats harbored T. (Herpetosoma) spp. PCR-amplified CATL gene fragments (253 bp) distinguish T. lewisi and T. lewisi-like from other trypanosomes, and allow for assessment of genetic diversity and relationships among T. (Herpetosoma) spp. Our molecular surveys corroborated worldwide high prevalence of T. lewisi, incriminating Mastomys natalensis as an important carrier of this species in Africa, and supported its spillover from invader Rattus spp. to native rodents in Brazil and Mozambique. THeCATL-PCR provided new insights on the accurate diagnosis and genetic repertoire of T. (Herpetosoma) spp. in rodent and non-rodent hosts, revealing a novel species of this subgenus in an African gerbil. Phylogenetic analysis based on CATL sequences from T. (Herpetosoma) spp. and other trypanosomes (amplified using pan-trypanosome primers) uncovered rodents harboring, beyond mammal trypanosomes of different subgenera, some species that clustered in the lizard-snake clade of trypanosomes. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/591905/1/Diagnosis%20and%20genetics%20analysis%20of%20the%20worldwide%20distributed%20rattus-borne%20Tryapanosoma%20lewisi%20and%20its%20allied%20species%20in%20blood%20and%20feas%20of%20rodents.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.meegid.2017.09.001 10.1016/j.meegid.2017.09.001 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2017.09.001 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.meegid.2017.09.001 |