Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control
Peste des petits ruminants (PPR) is a highly contagious and devastating viral disease affecting mainly sheep and goats, but also a large number of wild species within the order Artiodactyla. A better understanding of PPR transmission dynamics in multi-host systems is necessary to efficiently control the disease, in particular where wildlife and livestock co-occur. Notably, the role of wildlife in PPR epidemiology is still not clearly understood. Non-invasive strategies to detect PPR infection without the need for animal handling could greatly facilitate research on PPR epidemiology and management of the disease in atypical hosts and in complex field situations. Here, we describe optimized methods for the direct detection of PPR virus genetic material and antigen in fecal samples. We use these methods to determine the detection window of PPR in fecal samples, and compare the sensitivity of these methods to standard invasive sampling and PPR diagnostic methods using field samples collected at a wildlife-livestock interface in Africa. Our results show that quantitative reverse transcription PCR (RT-QPCR) amplification of PPRV from fecal swabs has good sensitivity in comparison to ocular swabs. Animals infected by PPRV could be identified relatively early on and during the whole course of infection based on fecal samples using RT-QPCR. Partial gene sequences could also be retrieved in some cases, from both fecal and ocular samples, providing important information about virus origin and relatedness to other PPRV strains. Non-invasive strategies for PPRV surveillance could provide important data to fill major gaps in our knowledge of the multi-host PPR epidemiology.
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Subjects: | L73 - Maladies des animaux, peste des petits ruminants, Artiodactyla, faune, bétail, épidémiologie, Transcription inverse, fèces, http://aims.fao.org/aos/agrovoc/c_16789, http://aims.fao.org/aos/agrovoc/c_643, http://aims.fao.org/aos/agrovoc/c_2821, http://aims.fao.org/aos/agrovoc/c_4397, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_35129, http://aims.fao.org/aos/agrovoc/c_2772, http://aims.fao.org/aos/agrovoc/c_7608, |
Online Access: | http://agritrop.cirad.fr/591824/ http://agritrop.cirad.fr/591824/1/Bataille_et_al-2019-Scientific_Reports.pdf |
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dig-cirad-fr-5918242024-01-29T01:47:55Z http://agritrop.cirad.fr/591824/ http://agritrop.cirad.fr/591824/ Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control. Bataille Arnaud, Kwiatek Olivier, Belfkhi Salima, Mounier Lucile, Parida Satya, Mahapatra Mana, Caron Alexandre, Chubwa Chobi Clement, Keyyu Julius, Kock Richard, Jones Bryony Anne, Libeau Geneviève. 2019. Scientific Reports, 9:4742, 8 p.https://doi.org/10.1038/s41598-019-41232-y <https://doi.org/10.1038/s41598-019-41232-y> Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control Bataille, Arnaud Kwiatek, Olivier Belfkhi, Salima Mounier, Lucile Parida, Satya Mahapatra, Mana Caron, Alexandre Chubwa, Chobi Clement Keyyu, Julius Kock, Richard Jones, Bryony Anne Libeau, Geneviève eng 2019 Scientific Reports L73 - Maladies des animaux peste des petits ruminants Artiodactyla faune bétail épidémiologie Transcription inverse fèces http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_643 http://aims.fao.org/aos/agrovoc/c_2821 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_2772 République-Unie de Tanzanie http://aims.fao.org/aos/agrovoc/c_7608 Peste des petits ruminants (PPR) is a highly contagious and devastating viral disease affecting mainly sheep and goats, but also a large number of wild species within the order Artiodactyla. A better understanding of PPR transmission dynamics in multi-host systems is necessary to efficiently control the disease, in particular where wildlife and livestock co-occur. Notably, the role of wildlife in PPR epidemiology is still not clearly understood. Non-invasive strategies to detect PPR infection without the need for animal handling could greatly facilitate research on PPR epidemiology and management of the disease in atypical hosts and in complex field situations. Here, we describe optimized methods for the direct detection of PPR virus genetic material and antigen in fecal samples. We use these methods to determine the detection window of PPR in fecal samples, and compare the sensitivity of these methods to standard invasive sampling and PPR diagnostic methods using field samples collected at a wildlife-livestock interface in Africa. Our results show that quantitative reverse transcription PCR (RT-QPCR) amplification of PPRV from fecal swabs has good sensitivity in comparison to ocular swabs. Animals infected by PPRV could be identified relatively early on and during the whole course of infection based on fecal samples using RT-QPCR. Partial gene sequences could also be retrieved in some cases, from both fecal and ocular samples, providing important information about virus origin and relatedness to other PPRV strains. Non-invasive strategies for PPRV surveillance could provide important data to fill major gaps in our knowledge of the multi-host PPR epidemiology. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/591824/1/Bataille_et_al-2019-Scientific_Reports.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1038/s41598-019-41232-y 10.1038/s41598-019-41232-y info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-019-41232-y info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/s41598-019-41232-y |
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L73 - Maladies des animaux peste des petits ruminants Artiodactyla faune bétail épidémiologie Transcription inverse fèces http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_643 http://aims.fao.org/aos/agrovoc/c_2821 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_2772 http://aims.fao.org/aos/agrovoc/c_7608 L73 - Maladies des animaux peste des petits ruminants Artiodactyla faune bétail épidémiologie Transcription inverse fèces http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_643 http://aims.fao.org/aos/agrovoc/c_2821 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_2772 http://aims.fao.org/aos/agrovoc/c_7608 |
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L73 - Maladies des animaux peste des petits ruminants Artiodactyla faune bétail épidémiologie Transcription inverse fèces http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_643 http://aims.fao.org/aos/agrovoc/c_2821 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_2772 http://aims.fao.org/aos/agrovoc/c_7608 L73 - Maladies des animaux peste des petits ruminants Artiodactyla faune bétail épidémiologie Transcription inverse fèces http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_643 http://aims.fao.org/aos/agrovoc/c_2821 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_2772 http://aims.fao.org/aos/agrovoc/c_7608 Bataille, Arnaud Kwiatek, Olivier Belfkhi, Salima Mounier, Lucile Parida, Satya Mahapatra, Mana Caron, Alexandre Chubwa, Chobi Clement Keyyu, Julius Kock, Richard Jones, Bryony Anne Libeau, Geneviève Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control |
description |
Peste des petits ruminants (PPR) is a highly contagious and devastating viral disease affecting mainly sheep and goats, but also a large number of wild species within the order Artiodactyla. A better understanding of PPR transmission dynamics in multi-host systems is necessary to efficiently control the disease, in particular where wildlife and livestock co-occur. Notably, the role of wildlife in PPR epidemiology is still not clearly understood. Non-invasive strategies to detect PPR infection without the need for animal handling could greatly facilitate research on PPR epidemiology and management of the disease in atypical hosts and in complex field situations. Here, we describe optimized methods for the direct detection of PPR virus genetic material and antigen in fecal samples. We use these methods to determine the detection window of PPR in fecal samples, and compare the sensitivity of these methods to standard invasive sampling and PPR diagnostic methods using field samples collected at a wildlife-livestock interface in Africa. Our results show that quantitative reverse transcription PCR (RT-QPCR) amplification of PPRV from fecal swabs has good sensitivity in comparison to ocular swabs. Animals infected by PPRV could be identified relatively early on and during the whole course of infection based on fecal samples using RT-QPCR. Partial gene sequences could also be retrieved in some cases, from both fecal and ocular samples, providing important information about virus origin and relatedness to other PPRV strains. Non-invasive strategies for PPRV surveillance could provide important data to fill major gaps in our knowledge of the multi-host PPR epidemiology. |
format |
article |
topic_facet |
L73 - Maladies des animaux peste des petits ruminants Artiodactyla faune bétail épidémiologie Transcription inverse fèces http://aims.fao.org/aos/agrovoc/c_16789 http://aims.fao.org/aos/agrovoc/c_643 http://aims.fao.org/aos/agrovoc/c_2821 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_35129 http://aims.fao.org/aos/agrovoc/c_2772 http://aims.fao.org/aos/agrovoc/c_7608 |
author |
Bataille, Arnaud Kwiatek, Olivier Belfkhi, Salima Mounier, Lucile Parida, Satya Mahapatra, Mana Caron, Alexandre Chubwa, Chobi Clement Keyyu, Julius Kock, Richard Jones, Bryony Anne Libeau, Geneviève |
author_facet |
Bataille, Arnaud Kwiatek, Olivier Belfkhi, Salima Mounier, Lucile Parida, Satya Mahapatra, Mana Caron, Alexandre Chubwa, Chobi Clement Keyyu, Julius Kock, Richard Jones, Bryony Anne Libeau, Geneviève |
author_sort |
Bataille, Arnaud |
title |
Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control |
title_short |
Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control |
title_full |
Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control |
title_fullStr |
Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control |
title_full_unstemmed |
Optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control |
title_sort |
optimization and evaluation of a non-invasive tool for peste des petits ruminants surveillance and control |
url |
http://agritrop.cirad.fr/591824/ http://agritrop.cirad.fr/591824/1/Bataille_et_al-2019-Scientific_Reports.pdf |
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