The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting
X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation.
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Subjects: | F30 - Génétique et amélioration des plantes, F62 - Physiologie végétale - Croissance et développement, Hevea brasiliensis, latex, relation plante eau, génome, homéostasie, glycérol, phylogénie, saignée, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_4214, http://aims.fao.org/aos/agrovoc/c_16147, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_33930, http://aims.fao.org/aos/agrovoc/c_3298, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_7611, http://aims.fao.org/aos/agrovoc/c_4027, |
Online Access: | http://agritrop.cirad.fr/590820/ http://agritrop.cirad.fr/590820/1/Lopez%202016.pdf |
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dig-cirad-fr-5908202024-01-29T01:34:32Z http://agritrop.cirad.fr/590820/ http://agritrop.cirad.fr/590820/ The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting. Lopez David, Ben Amira Maroua, Brown Daniel, Muries Beatriz, Brunel-Michac Nicole, Bourgerie Sylvain, Porcheron Benoit, Lemoine Remi, Chrestin Hervé, Mollison Ewan, Di Cola Alessandra, Frigerio Lorenzo, Julien Jean-Louis, Gousset-Dupont Aurélie, Fumanal Boris, Label Philippe, Pujade-Renaud Valérie, Auguin Daniel, Venisse Jean-Stéphane. 2016. Plant Molecular Biology, 91 (4-5) : 375-396.https://doi.org/10.1007/s11103-016-0462-y <https://doi.org/10.1007/s11103-016-0462-y> The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting Lopez, David Ben Amira, Maroua Brown, Daniel Muries, Beatriz Brunel-Michac, Nicole Bourgerie, Sylvain Porcheron, Benoit Lemoine, Remi Chrestin, Hervé Mollison, Ewan Di Cola, Alessandra Frigerio, Lorenzo Julien, Jean-Louis Gousset-Dupont, Aurélie Fumanal, Boris Label, Philippe Pujade-Renaud, Valérie Auguin, Daniel Venisse, Jean-Stéphane eng 2016 Plant Molecular Biology F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis latex relation plante eau génome homéostasie glycérol phylogénie saignée http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_16147 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_3298 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_7611 Côte d'Ivoire http://aims.fao.org/aos/agrovoc/c_4027 X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/590820/1/Lopez%202016.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s11103-016-0462-y 10.1007/s11103-016-0462-y info:eu-repo/semantics/altIdentifier/doi/10.1007/s11103-016-0462-y info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s11103-016-0462-y |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis latex relation plante eau génome homéostasie glycérol phylogénie saignée http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_16147 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_3298 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_7611 http://aims.fao.org/aos/agrovoc/c_4027 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis latex relation plante eau génome homéostasie glycérol phylogénie saignée http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_16147 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_3298 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_7611 http://aims.fao.org/aos/agrovoc/c_4027 |
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F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis latex relation plante eau génome homéostasie glycérol phylogénie saignée http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_16147 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_3298 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_7611 http://aims.fao.org/aos/agrovoc/c_4027 F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis latex relation plante eau génome homéostasie glycérol phylogénie saignée http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_16147 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_3298 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_7611 http://aims.fao.org/aos/agrovoc/c_4027 Lopez, David Ben Amira, Maroua Brown, Daniel Muries, Beatriz Brunel-Michac, Nicole Bourgerie, Sylvain Porcheron, Benoit Lemoine, Remi Chrestin, Hervé Mollison, Ewan Di Cola, Alessandra Frigerio, Lorenzo Julien, Jean-Louis Gousset-Dupont, Aurélie Fumanal, Boris Label, Philippe Pujade-Renaud, Valérie Auguin, Daniel Venisse, Jean-Stéphane The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting |
description |
X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation. |
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article |
topic_facet |
F30 - Génétique et amélioration des plantes F62 - Physiologie végétale - Croissance et développement Hevea brasiliensis latex relation plante eau génome homéostasie glycérol phylogénie saignée http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_16147 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_33930 http://aims.fao.org/aos/agrovoc/c_3298 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_7611 http://aims.fao.org/aos/agrovoc/c_4027 |
author |
Lopez, David Ben Amira, Maroua Brown, Daniel Muries, Beatriz Brunel-Michac, Nicole Bourgerie, Sylvain Porcheron, Benoit Lemoine, Remi Chrestin, Hervé Mollison, Ewan Di Cola, Alessandra Frigerio, Lorenzo Julien, Jean-Louis Gousset-Dupont, Aurélie Fumanal, Boris Label, Philippe Pujade-Renaud, Valérie Auguin, Daniel Venisse, Jean-Stéphane |
author_facet |
Lopez, David Ben Amira, Maroua Brown, Daniel Muries, Beatriz Brunel-Michac, Nicole Bourgerie, Sylvain Porcheron, Benoit Lemoine, Remi Chrestin, Hervé Mollison, Ewan Di Cola, Alessandra Frigerio, Lorenzo Julien, Jean-Louis Gousset-Dupont, Aurélie Fumanal, Boris Label, Philippe Pujade-Renaud, Valérie Auguin, Daniel Venisse, Jean-Stéphane |
author_sort |
Lopez, David |
title |
The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting |
title_short |
The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting |
title_full |
The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting |
title_fullStr |
The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting |
title_full_unstemmed |
The Hevea brasiliensis XIP aquaporin subfamily: Genomic, structural and functional characterizations with relevance to intensive latex harvesting |
title_sort |
hevea brasiliensis xip aquaporin subfamily: genomic, structural and functional characterizations with relevance to intensive latex harvesting |
url |
http://agritrop.cirad.fr/590820/ http://agritrop.cirad.fr/590820/1/Lopez%202016.pdf |
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