Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus
The Ribosomal DNAs of Helianthus annuus and H. argophyllus were analysed. Total DNA from single individuals of six cultivated lines, one wild ecotype ofH. annuus, and three ecotypes of H. argophyllus, were digested with various restriction enzymes. Hybridisation of Southern blots with sunflower ribosomal probes containing most of the interspacer regions (R3) or the 25 s coding region (R2) reveals different patterns from those expected: while no difference between H. annuus and H. argophyllus had been observed in previous rDNA RFLP analysis, our study clearly distinguished the two species on the basis of two different patterns when using R3 and BamHI, BstYI, or EcoRI/BamHI. Furthermore, the sum of the fragment weights of the BamHI restriction patterns was much greater than that of the rDNA entire unit-weight space. The co-existence of different rDNA units within single individuals is proposed as a model to explain these results. Four rDNA units were distinguished, which differed in their state of methylation and by the presence of mutations at two BamRI restriction sites. H. annuus individuals displayed two types of rDNA units while H. argophyllus individuals displayed four types.
Main Authors: | , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | F30 - Génétique et amélioration des plantes, Helianthus annuus, adn ribosomal, marqueur génétique, RFLP, méthylation, polymorphisme génétique, cartographie chromosomique, hybridation d'ADN, http://aims.fao.org/aos/agrovoc/c_3539, http://aims.fao.org/aos/agrovoc/c_1354098656894, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_34255, http://aims.fao.org/aos/agrovoc/c_24872, http://aims.fao.org/aos/agrovoc/c_24031, http://aims.fao.org/aos/agrovoc/c_331006, http://aims.fao.org/aos/agrovoc/c_33478, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/589573/ http://agritrop.cirad.fr/589573/1/TAG_1996.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-589573 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5895732024-01-29T01:22:13Z http://agritrop.cirad.fr/589573/ http://agritrop.cirad.fr/589573/ Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus. Cuellar Térésa, Bella José L., Belhassen Eric. 1996. Theoretical and Applied Genetics, 93 (5-6) : 794-800.https://doi.org/10.1007/BF00224078 <https://doi.org/10.1007/BF00224078> Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus Cuellar, Térésa Bella, José L. Belhassen, Eric eng 1996 Theoretical and Applied Genetics F30 - Génétique et amélioration des plantes Helianthus annuus adn ribosomal marqueur génétique RFLP méthylation polymorphisme génétique cartographie chromosomique hybridation d'ADN http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_1354098656894 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_331006 http://aims.fao.org/aos/agrovoc/c_33478 France http://aims.fao.org/aos/agrovoc/c_3081 The Ribosomal DNAs of Helianthus annuus and H. argophyllus were analysed. Total DNA from single individuals of six cultivated lines, one wild ecotype ofH. annuus, and three ecotypes of H. argophyllus, were digested with various restriction enzymes. Hybridisation of Southern blots with sunflower ribosomal probes containing most of the interspacer regions (R3) or the 25 s coding region (R2) reveals different patterns from those expected: while no difference between H. annuus and H. argophyllus had been observed in previous rDNA RFLP analysis, our study clearly distinguished the two species on the basis of two different patterns when using R3 and BamHI, BstYI, or EcoRI/BamHI. Furthermore, the sum of the fragment weights of the BamHI restriction patterns was much greater than that of the rDNA entire unit-weight space. The co-existence of different rDNA units within single individuals is proposed as a model to explain these results. Four rDNA units were distinguished, which differed in their state of methylation and by the presence of mutations at two BamRI restriction sites. H. annuus individuals displayed two types of rDNA units while H. argophyllus individuals displayed four types. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/589573/1/TAG_1996.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/BF00224078 10.1007/BF00224078 info:eu-repo/semantics/altIdentifier/doi/10.1007/BF00224078 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/BF00224078 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
F30 - Génétique et amélioration des plantes Helianthus annuus adn ribosomal marqueur génétique RFLP méthylation polymorphisme génétique cartographie chromosomique hybridation d'ADN http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_1354098656894 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_331006 http://aims.fao.org/aos/agrovoc/c_33478 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes Helianthus annuus adn ribosomal marqueur génétique RFLP méthylation polymorphisme génétique cartographie chromosomique hybridation d'ADN http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_1354098656894 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_331006 http://aims.fao.org/aos/agrovoc/c_33478 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
F30 - Génétique et amélioration des plantes Helianthus annuus adn ribosomal marqueur génétique RFLP méthylation polymorphisme génétique cartographie chromosomique hybridation d'ADN http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_1354098656894 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_331006 http://aims.fao.org/aos/agrovoc/c_33478 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes Helianthus annuus adn ribosomal marqueur génétique RFLP méthylation polymorphisme génétique cartographie chromosomique hybridation d'ADN http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_1354098656894 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_331006 http://aims.fao.org/aos/agrovoc/c_33478 http://aims.fao.org/aos/agrovoc/c_3081 Cuellar, Térésa Bella, José L. Belhassen, Eric Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus |
description |
The Ribosomal DNAs of Helianthus annuus and H. argophyllus were analysed. Total DNA from single individuals of six cultivated lines, one wild ecotype ofH. annuus, and three ecotypes of H. argophyllus, were digested with various restriction enzymes. Hybridisation of Southern blots with sunflower ribosomal probes containing most of the interspacer regions (R3) or the 25 s coding region (R2) reveals different patterns from those expected: while no difference between H. annuus and H. argophyllus had been observed in previous rDNA RFLP analysis, our study clearly distinguished the two species on the basis of two different patterns when using R3 and BamHI, BstYI, or EcoRI/BamHI. Furthermore, the sum of the fragment weights of the BamHI restriction patterns was much greater than that of the rDNA entire unit-weight space. The co-existence of different rDNA units within single individuals is proposed as a model to explain these results. Four rDNA units were distinguished, which differed in their state of methylation and by the presence of mutations at two BamRI restriction sites. H. annuus individuals displayed two types of rDNA units while H. argophyllus individuals displayed four types. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Helianthus annuus adn ribosomal marqueur génétique RFLP méthylation polymorphisme génétique cartographie chromosomique hybridation d'ADN http://aims.fao.org/aos/agrovoc/c_3539 http://aims.fao.org/aos/agrovoc/c_1354098656894 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_34255 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_24031 http://aims.fao.org/aos/agrovoc/c_331006 http://aims.fao.org/aos/agrovoc/c_33478 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Cuellar, Térésa Bella, José L. Belhassen, Eric |
author_facet |
Cuellar, Térésa Bella, José L. Belhassen, Eric |
author_sort |
Cuellar, Térésa |
title |
Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus |
title_short |
Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus |
title_full |
Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus |
title_fullStr |
Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus |
title_full_unstemmed |
Intra-individual heterogeneity of rDNA allows the distinction between two closely related species in the Genus Helianthus |
title_sort |
intra-individual heterogeneity of rdna allows the distinction between two closely related species in the genus helianthus |
url |
http://agritrop.cirad.fr/589573/ http://agritrop.cirad.fr/589573/1/TAG_1996.pdf |
work_keys_str_mv |
AT cuellarteresa intraindividualheterogeneityofrdnaallowsthedistinctionbetweentwocloselyrelatedspeciesinthegenushelianthus AT bellajosel intraindividualheterogeneityofrdnaallowsthedistinctionbetweentwocloselyrelatedspeciesinthegenushelianthus AT belhasseneric intraindividualheterogeneityofrdnaallowsthedistinctionbetweentwocloselyrelatedspeciesinthegenushelianthus |
_version_ |
1792499578772127744 |