Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance
The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop knowledge on the mode of inheritance in the intergeneric tetraploid hybrids. We assessed the meiotic behavior of an intergeneric tetraploid somatic hybrid resulting from symmetric protoplast fusion of diploid Citrus reticulata and diploid Poncirus trifoliata. The analysis was based on the segregation patterns of 16 SSR markers and 9 newly developed centromeric/pericentromeric SNP markers, representing all nine linkage groups of the Citrus genetic map. We found strong but incomplete preferential pairing between homologues of the same ancestral genome. The proportion of gametes that can be explained by random meiotic chromosome associations (τ) varied significantly between chromosomes, from 0.09 ± 0.02 to 0.47 ± 0.09, respectively, in chromosome 2 and 1. This intermediate inheritance between strict disomy and tetrasomy, with global preferential disomic tendency, resulted in a high level of intergeneric heterozygosity of the diploid gametes. Although limited, intergeneric recombinations occurred, whose observed rates, ranging from 0.09 to 0.29, respectively, in chromosome 2 and 1, were significantly correlated with τ. Such inheritance is of particular interest for rootstock breeding because a large part of the multi-trait value selected at the teraploid parent level is transmitted to the progeny, while the potential for some intergeneric recombination offers opportunities for generating plants with novel allelic combinations that can be targeted by selection.
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dig-cirad-fr-5894152022-08-03T16:03:20Z http://agritrop.cirad.fr/589415/ http://agritrop.cirad.fr/589415/ Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance. Kamiri Mourad, Stift Marc, Costantino Gilles, Dambier Dominique, Kabbage Tariq, Ollitrault Patrick, Froelicher Yann. 2018. Frontiers in Plant Science, 9:1557, 12 p.https://doi.org/10.3389/fpls.2018.01557 <https://doi.org/10.3389/fpls.2018.01557> Researchers Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance Kamiri, Mourad Stift, Marc Costantino, Gilles Dambier, Dominique Kabbage, Tariq Ollitrault, Patrick Froelicher, Yann eng 2018 Frontiers in Plant Science F30 - Génétique et amélioration des plantes Corse France http://aims.fao.org/aos/agrovoc/c_1907 http://aims.fao.org/aos/agrovoc/c_3081 The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop knowledge on the mode of inheritance in the intergeneric tetraploid hybrids. We assessed the meiotic behavior of an intergeneric tetraploid somatic hybrid resulting from symmetric protoplast fusion of diploid Citrus reticulata and diploid Poncirus trifoliata. The analysis was based on the segregation patterns of 16 SSR markers and 9 newly developed centromeric/pericentromeric SNP markers, representing all nine linkage groups of the Citrus genetic map. We found strong but incomplete preferential pairing between homologues of the same ancestral genome. The proportion of gametes that can be explained by random meiotic chromosome associations (τ) varied significantly between chromosomes, from 0.09 ± 0.02 to 0.47 ± 0.09, respectively, in chromosome 2 and 1. This intermediate inheritance between strict disomy and tetrasomy, with global preferential disomic tendency, resulted in a high level of intergeneric heterozygosity of the diploid gametes. Although limited, intergeneric recombinations occurred, whose observed rates, ranging from 0.09 to 0.29, respectively, in chromosome 2 and 1, were significantly correlated with τ. Such inheritance is of particular interest for rootstock breeding because a large part of the multi-trait value selected at the teraploid parent level is transmitted to the progeny, while the potential for some intergeneric recombination offers opportunities for generating plants with novel allelic combinations that can be targeted by selection. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/589415/1/fpls-09-01557%283%29.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.3389/fpls.2018.01557 10.3389/fpls.2018.01557 info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2018.01557 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3389/fpls.2018.01557 |
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F30 - Génétique et amélioration des plantes http://aims.fao.org/aos/agrovoc/c_1907 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes http://aims.fao.org/aos/agrovoc/c_1907 http://aims.fao.org/aos/agrovoc/c_3081 |
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F30 - Génétique et amélioration des plantes http://aims.fao.org/aos/agrovoc/c_1907 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes http://aims.fao.org/aos/agrovoc/c_1907 http://aims.fao.org/aos/agrovoc/c_3081 Kamiri, Mourad Stift, Marc Costantino, Gilles Dambier, Dominique Kabbage, Tariq Ollitrault, Patrick Froelicher, Yann Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance |
description |
The creation of intergeneric somatic hybrids between Citrus and Poncirus is an efficient approach for citrus rootstock breeding, offering the possibility of combining beneficial traits from both genera into novel rootstock lineages. These somatic hybrids are also used as parents for further tetraploid sexual breeding. In order to optimize these latter breeding schemes, it is essential to develop knowledge on the mode of inheritance in the intergeneric tetraploid hybrids. We assessed the meiotic behavior of an intergeneric tetraploid somatic hybrid resulting from symmetric protoplast fusion of diploid Citrus reticulata and diploid Poncirus trifoliata. The analysis was based on the segregation patterns of 16 SSR markers and 9 newly developed centromeric/pericentromeric SNP markers, representing all nine linkage groups of the Citrus genetic map. We found strong but incomplete preferential pairing between homologues of the same ancestral genome. The proportion of gametes that can be explained by random meiotic chromosome associations (τ) varied significantly between chromosomes, from 0.09 ± 0.02 to 0.47 ± 0.09, respectively, in chromosome 2 and 1. This intermediate inheritance between strict disomy and tetrasomy, with global preferential disomic tendency, resulted in a high level of intergeneric heterozygosity of the diploid gametes. Although limited, intergeneric recombinations occurred, whose observed rates, ranging from 0.09 to 0.29, respectively, in chromosome 2 and 1, were significantly correlated with τ. Such inheritance is of particular interest for rootstock breeding because a large part of the multi-trait value selected at the teraploid parent level is transmitted to the progeny, while the potential for some intergeneric recombination offers opportunities for generating plants with novel allelic combinations that can be targeted by selection. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes http://aims.fao.org/aos/agrovoc/c_1907 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Kamiri, Mourad Stift, Marc Costantino, Gilles Dambier, Dominique Kabbage, Tariq Ollitrault, Patrick Froelicher, Yann |
author_facet |
Kamiri, Mourad Stift, Marc Costantino, Gilles Dambier, Dominique Kabbage, Tariq Ollitrault, Patrick Froelicher, Yann |
author_sort |
Kamiri, Mourad |
title |
Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance |
title_short |
Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance |
title_full |
Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance |
title_fullStr |
Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance |
title_full_unstemmed |
Preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (Citrus reticulata + Poncirus trifoliata)revealed by molecular marker inheritance |
title_sort |
preferential homologous chromosome pairing in a tetraploid intergeneric somatic hybrid (citrus reticulata + poncirus trifoliata)revealed by molecular marker inheritance |
url |
http://agritrop.cirad.fr/589415/ http://agritrop.cirad.fr/589415/1/fpls-09-01557%283%29.pdf |
work_keys_str_mv |
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1758025900061884416 |