Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra
Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrynolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these γ-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified γ-lactone, paving the way for future studies in biosynthesis.
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Subjects: | K50 - Technologie des produits forestiers, F60 - Physiologie et biochimie végétale, U30 - Méthodes de recherche, bois, forêt tropicale humide, métabolite, bois de coeur, spectrométrie de masse, Lauraceae, http://aims.fao.org/aos/agrovoc/c_8421, http://aims.fao.org/aos/agrovoc/c_7976, http://aims.fao.org/aos/agrovoc/c_4770, http://aims.fao.org/aos/agrovoc/c_3518, http://aims.fao.org/aos/agrovoc/c_12421, http://aims.fao.org/aos/agrovoc/c_4225, http://aims.fao.org/aos/agrovoc/c_3093, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/588713/ http://agritrop.cirad.fr/588713/7/acs.analchem.8b01157.pdf |
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dig-cirad-fr-5887132024-01-29T01:10:38Z http://agritrop.cirad.fr/588713/ http://agritrop.cirad.fr/588713/ Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra. Fu Tingting, Touboul David, Della Negra Serge, Houël Emeline, Amusant Nadine, Duplais Christophe, Fisher Gregory L., Brunelle Alain. 2018. Analytical Chemistry, 90 (12) : 7535-7543.https://doi.org/10.1021/acs.analchem.8b01157 <https://doi.org/10.1021/acs.analchem.8b01157> Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra Fu, Tingting Touboul, David Della Negra, Serge Houël, Emeline Amusant, Nadine Duplais, Christophe Fisher, Gregory L. Brunelle, Alain eng 2018 Analytical Chemistry K50 - Technologie des produits forestiers F60 - Physiologie et biochimie végétale U30 - Méthodes de recherche bois forêt tropicale humide métabolite bois de coeur spectrométrie de masse Lauraceae http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_7976 http://aims.fao.org/aos/agrovoc/c_4770 http://aims.fao.org/aos/agrovoc/c_3518 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4225 Guyane française France http://aims.fao.org/aos/agrovoc/c_3093 http://aims.fao.org/aos/agrovoc/c_3081 Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrynolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these γ-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified γ-lactone, paving the way for future studies in biosynthesis. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/588713/7/acs.analchem.8b01157.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1021/acs.analchem.8b01157 10.1021/acs.analchem.8b01157 info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.analchem.8b01157 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1021/acs.analchem.8b01157 |
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K50 - Technologie des produits forestiers F60 - Physiologie et biochimie végétale U30 - Méthodes de recherche bois forêt tropicale humide métabolite bois de coeur spectrométrie de masse Lauraceae http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_7976 http://aims.fao.org/aos/agrovoc/c_4770 http://aims.fao.org/aos/agrovoc/c_3518 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4225 http://aims.fao.org/aos/agrovoc/c_3093 http://aims.fao.org/aos/agrovoc/c_3081 K50 - Technologie des produits forestiers F60 - Physiologie et biochimie végétale U30 - Méthodes de recherche bois forêt tropicale humide métabolite bois de coeur spectrométrie de masse Lauraceae http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_7976 http://aims.fao.org/aos/agrovoc/c_4770 http://aims.fao.org/aos/agrovoc/c_3518 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4225 http://aims.fao.org/aos/agrovoc/c_3093 http://aims.fao.org/aos/agrovoc/c_3081 |
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K50 - Technologie des produits forestiers F60 - Physiologie et biochimie végétale U30 - Méthodes de recherche bois forêt tropicale humide métabolite bois de coeur spectrométrie de masse Lauraceae http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_7976 http://aims.fao.org/aos/agrovoc/c_4770 http://aims.fao.org/aos/agrovoc/c_3518 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4225 http://aims.fao.org/aos/agrovoc/c_3093 http://aims.fao.org/aos/agrovoc/c_3081 K50 - Technologie des produits forestiers F60 - Physiologie et biochimie végétale U30 - Méthodes de recherche bois forêt tropicale humide métabolite bois de coeur spectrométrie de masse Lauraceae http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_7976 http://aims.fao.org/aos/agrovoc/c_4770 http://aims.fao.org/aos/agrovoc/c_3518 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4225 http://aims.fao.org/aos/agrovoc/c_3093 http://aims.fao.org/aos/agrovoc/c_3081 Fu, Tingting Touboul, David Della Negra, Serge Houël, Emeline Amusant, Nadine Duplais, Christophe Fisher, Gregory L. Brunelle, Alain Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra |
description |
Driven by a necessity for confident molecular identification at high spatial resolution, a new time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem mass spectrometry (tandem MS) imaging instrument has been recently developed. In this paper, the superior MS/MS spectrometry and imaging capability of this new tool is shown for natural product study. For the first time, via in situ analysis of the bioactive metabolites rubrynolide and rubrenolide in Amazonian tree species Sextonia rubra (Lauraceae), we were able both to analyze and to image by tandem MS the molecular products of natural biosynthesis. Despite the low abundance of the metabolites in the wood sample(s), efficient MS/MS analysis of these γ-lactone compounds was achieved, providing high confidence in the identification and localization. In addition, tandem MS imaging minimized the mass interferences and revealed specific localization of these metabolites primarily in the ray parenchyma cells but also in certain oil cells and, further, revealed the presence of previously unidentified γ-lactone, paving the way for future studies in biosynthesis. |
format |
article |
topic_facet |
K50 - Technologie des produits forestiers F60 - Physiologie et biochimie végétale U30 - Méthodes de recherche bois forêt tropicale humide métabolite bois de coeur spectrométrie de masse Lauraceae http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_7976 http://aims.fao.org/aos/agrovoc/c_4770 http://aims.fao.org/aos/agrovoc/c_3518 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4225 http://aims.fao.org/aos/agrovoc/c_3093 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Fu, Tingting Touboul, David Della Negra, Serge Houël, Emeline Amusant, Nadine Duplais, Christophe Fisher, Gregory L. Brunelle, Alain |
author_facet |
Fu, Tingting Touboul, David Della Negra, Serge Houël, Emeline Amusant, Nadine Duplais, Christophe Fisher, Gregory L. Brunelle, Alain |
author_sort |
Fu, Tingting |
title |
Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra |
title_short |
Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra |
title_full |
Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra |
title_fullStr |
Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra |
title_full_unstemmed |
Tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in Amazonian tree species Sextonia rubra |
title_sort |
tandem mass spectrometry imaging and in situ characterization of bioactive wood metabolites in amazonian tree species sextonia rubra |
url |
http://agritrop.cirad.fr/588713/ http://agritrop.cirad.fr/588713/7/acs.analchem.8b01157.pdf |
work_keys_str_mv |
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