Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines
Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group. Conclusions: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology.
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Subjects: | L73 - Maladies des animaux, L20 - Écologie animale, P01 - Conservation de la nature et ressources foncières, http://aims.fao.org/aos/agrovoc/c_603, |
Online Access: | http://agritrop.cirad.fr/587974/ http://agritrop.cirad.fr/587974/1/Erratum%20to%20Molecular%20identification_Guillemi_2016.pdf |
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dig-cirad-fr-5879742022-01-19T07:15:47Z http://agritrop.cirad.fr/587974/ http://agritrop.cirad.fr/587974/ Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines. Guillemi Eliana C., De la Fourniere Sofía, Orozco Marcela, Martinez Jorge Peña, Correa Elena, Fernandez Javier, Lopez Arias Ludmila, Paoletta Martina, Corona Belkis, Pinarello Valérie, Wilkowsky Silvina E., Farber Marisa D.. 2016. Parasites and Vectors, 9:358, 9 p.https://doi.org/10.1186/s13071-016-1555-9 <https://doi.org/10.1186/s13071-016-1555-9> Researchers Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines Guillemi, Eliana C. De la Fourniere, Sofía Orozco, Marcela Martinez, Jorge Peña Correa, Elena Fernandez, Javier Lopez Arias, Ludmila Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina E. Farber, Marisa D. eng 2016 Parasites and Vectors L73 - Maladies des animaux L20 - Écologie animale P01 - Conservation de la nature et ressources foncières Argentine http://aims.fao.org/aos/agrovoc/c_603 Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group. Conclusions: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/587974/1/Erratum%20to%20Molecular%20identification_Guillemi_2016.pdf text cc_0 info:eu-repo/semantics/openAccess https://creativecommons.org/publicdomain/zero/1.0/ https://doi.org/10.1186/s13071-016-1555-9 10.1186/s13071-016-1555-9 info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-016-1555-9 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/s13071-016-1555-9 |
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L73 - Maladies des animaux L20 - Écologie animale P01 - Conservation de la nature et ressources foncières http://aims.fao.org/aos/agrovoc/c_603 L73 - Maladies des animaux L20 - Écologie animale P01 - Conservation de la nature et ressources foncières http://aims.fao.org/aos/agrovoc/c_603 |
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L73 - Maladies des animaux L20 - Écologie animale P01 - Conservation de la nature et ressources foncières http://aims.fao.org/aos/agrovoc/c_603 L73 - Maladies des animaux L20 - Écologie animale P01 - Conservation de la nature et ressources foncières http://aims.fao.org/aos/agrovoc/c_603 Guillemi, Eliana C. De la Fourniere, Sofía Orozco, Marcela Martinez, Jorge Peña Correa, Elena Fernandez, Javier Lopez Arias, Ludmila Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina E. Farber, Marisa D. Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
description |
Background: Anaplasma marginale is a well-known cattle pathogen of tropical and subtropical world regions. Even though, this obligate intracellular bacterium has been reported in other host species different than bovine, it has never been documented in Myrmecophaga tridactyla (giant anteater) or Hippocamelus antisense (taruca), which are two native endangered species. Methods: Samples from two sick wild animals: a Myrmecophaga tridactyla (blood) and a Hippocamelus antisense (blood and serum) were studied for the presence of A. marginale DNA through msp5 gene fragment amplification. Further characterization was done through MSP1a tandem repeats analysis and MLST scheme and the genetic relationship among previously characterized A. marginale sequences were studied by applying, eBURST algorithm and AMOVA analysis. Results: Anaplasma marginale DNA was identified in the Myrmecophaga tridactyla and Hippocamelus antisense samples. Through molecular markers, we identified an identical genotype in both animals that was not previously reported in bovine host. The analysis through eBURST and AMOVA revealed no differentiation between the taruca/anteater isolate and the bovine group. Conclusions: In the present publication we report the identification of A. marginale DNA in a novel ruminant (Hippocamelus antisense) and non-ruminant (Myrmecophaga tridactyla) host species. Genotyping analysis of isolates demonstrated the close relatedness of the new isolate with the circulation population of A. marginale in livestock. Further analysis is needed to understand whether these two hosts contribute to the anaplasmosis epidemiology. |
format |
article |
topic_facet |
L73 - Maladies des animaux L20 - Écologie animale P01 - Conservation de la nature et ressources foncières http://aims.fao.org/aos/agrovoc/c_603 |
author |
Guillemi, Eliana C. De la Fourniere, Sofía Orozco, Marcela Martinez, Jorge Peña Correa, Elena Fernandez, Javier Lopez Arias, Ludmila Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina E. Farber, Marisa D. |
author_facet |
Guillemi, Eliana C. De la Fourniere, Sofía Orozco, Marcela Martinez, Jorge Peña Correa, Elena Fernandez, Javier Lopez Arias, Ludmila Paoletta, Martina Corona, Belkis Pinarello, Valérie Wilkowsky, Silvina E. Farber, Marisa D. |
author_sort |
Guillemi, Eliana C. |
title |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
title_short |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
title_full |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
title_fullStr |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
title_full_unstemmed |
Molecular identification of Anaplasma marginale in two autochthonous South American wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
title_sort |
molecular identification of anaplasma marginale in two autochthonous south american wild species revealed an identical new genotype and its phylogenetic relationship with those of bovines |
url |
http://agritrop.cirad.fr/587974/ http://agritrop.cirad.fr/587974/1/Erratum%20to%20Molecular%20identification_Guillemi_2016.pdf |
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