MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees
Major intrinsic proteins (MIP) are characterized by a transmembrane pore-type architecture that facilitates transport across biomembranes of water and a variety of low molecular weight solutes. They are found in all parts of life, with remarkable protein diversity. Very little is known about MIP from fungi. And yet, it can legitimately be stated that MIP are pivotal molecular components in the privileged relationships fungi enjoy with plants or soil fauna in various environments. To date, MIP have never been studied in a mycoparasitism situation. In this study, the diversity, expression and functional prediction of MIP from the genus Trichoderma were investigated. Trichoderma spp. genomes have at least seven aquaporin genes. Based on a phylogenetic analysis of the translated sequences, members were assigned to the AQP, AQGP and XIP subfamilies. In in vitro and in planta assays with T. harzianum strain Ths97, expression analyses showed that four genes were constitutively expressed. In a mycoparasitic context with Fusarium solani, the causative agent of fusarium dieback on olive tree roots, these genes were up-regulated. This response is of particular interest in analyzing the MIP promoter cis-regulatory motifs, most of which are involved in various carbon and nitrogen metabolisms. Structural analyses provide new insights into the possible role of structural checkpoints by which these members transport water, H2O2, glycerol and, more generally, linear polyols across the membranes. Taken together, these results provide the first evidence that MIP may play a key role in Trichoderma mycoparasitism lifestyle.
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dig-cirad-fr-5876872021-11-09T09:38:21Z http://agritrop.cirad.fr/587687/ http://agritrop.cirad.fr/587687/ MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees. Ben Amira Maroua, Mom Robin, Lopez David, Chaar Hatem, Khouaja Ali, Pujade-Renaud Valérie, Fumanal Boris, Gousset-Dupont Aurélie, Bronner Gisèle, Label Philippe, Julien Jean-Louis, Triki Ali Mohamed, Auguin Daniel, Venisse Jean-Stéphane. 2018. PloS One, 13 (3):e0193760, 23 p.https://doi.org/10.1371/journal.pone.0193760 <https://doi.org/10.1371/journal.pone.0193760> Researchers MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees Ben Amira, Maroua Mom, Robin Lopez, David Chaar, Hatem Khouaja, Ali Pujade-Renaud, Valérie Fumanal, Boris Gousset-Dupont, Aurélie Bronner, Gisèle Label, Philippe Julien, Jean-Louis Triki, Ali Mohamed Auguin, Daniel Venisse, Jean-Stéphane eng 2018 PloS One H20 - Maladies des plantes Major intrinsic proteins (MIP) are characterized by a transmembrane pore-type architecture that facilitates transport across biomembranes of water and a variety of low molecular weight solutes. They are found in all parts of life, with remarkable protein diversity. Very little is known about MIP from fungi. And yet, it can legitimately be stated that MIP are pivotal molecular components in the privileged relationships fungi enjoy with plants or soil fauna in various environments. To date, MIP have never been studied in a mycoparasitism situation. In this study, the diversity, expression and functional prediction of MIP from the genus Trichoderma were investigated. Trichoderma spp. genomes have at least seven aquaporin genes. Based on a phylogenetic analysis of the translated sequences, members were assigned to the AQP, AQGP and XIP subfamilies. In in vitro and in planta assays with T. harzianum strain Ths97, expression analyses showed that four genes were constitutively expressed. In a mycoparasitic context with Fusarium solani, the causative agent of fusarium dieback on olive tree roots, these genes were up-regulated. This response is of particular interest in analyzing the MIP promoter cis-regulatory motifs, most of which are involved in various carbon and nitrogen metabolisms. Structural analyses provide new insights into the possible role of structural checkpoints by which these members transport water, H2O2, glycerol and, more generally, linear polyols across the membranes. Taken together, these results provide the first evidence that MIP may play a key role in Trichoderma mycoparasitism lifestyle. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/587687/1/journal.pone.0193760.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1371/journal.pone.0193760 10.1371/journal.pone.0193760 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0193760 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pone.0193760 info:eu-repo/semantics/dataset/purl/https://figshare.com/articles/MIP_diversity_from_i_Trichoderma_i_Structural_considerations_and_transcriptional_modulation_during_mycoparasitic_association_with_i_Fusarium_solani_i_olive_trees/5988817 |
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H20 - Maladies des plantes H20 - Maladies des plantes Ben Amira, Maroua Mom, Robin Lopez, David Chaar, Hatem Khouaja, Ali Pujade-Renaud, Valérie Fumanal, Boris Gousset-Dupont, Aurélie Bronner, Gisèle Label, Philippe Julien, Jean-Louis Triki, Ali Mohamed Auguin, Daniel Venisse, Jean-Stéphane MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees |
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Major intrinsic proteins (MIP) are characterized by a transmembrane pore-type architecture that facilitates transport across biomembranes of water and a variety of low molecular weight solutes. They are found in all parts of life, with remarkable protein diversity. Very little is known about MIP from fungi. And yet, it can legitimately be stated that MIP are pivotal molecular components in the privileged relationships fungi enjoy with plants or soil fauna in various environments. To date, MIP have never been studied in a mycoparasitism situation. In this study, the diversity, expression and functional prediction of MIP from the genus Trichoderma were investigated. Trichoderma spp. genomes have at least seven aquaporin genes. Based on a phylogenetic analysis of the translated sequences, members were assigned to the AQP, AQGP and XIP subfamilies. In in vitro and in planta assays with T. harzianum strain Ths97, expression analyses showed that four genes were constitutively expressed. In a mycoparasitic context with Fusarium solani, the causative agent of fusarium dieback on olive tree roots, these genes were up-regulated. This response is of particular interest in analyzing the MIP promoter cis-regulatory motifs, most of which are involved in various carbon and nitrogen metabolisms. Structural analyses provide new insights into the possible role of structural checkpoints by which these members transport water, H2O2, glycerol and, more generally, linear polyols across the membranes. Taken together, these results provide the first evidence that MIP may play a key role in Trichoderma mycoparasitism lifestyle. |
format |
article |
topic_facet |
H20 - Maladies des plantes |
author |
Ben Amira, Maroua Mom, Robin Lopez, David Chaar, Hatem Khouaja, Ali Pujade-Renaud, Valérie Fumanal, Boris Gousset-Dupont, Aurélie Bronner, Gisèle Label, Philippe Julien, Jean-Louis Triki, Ali Mohamed Auguin, Daniel Venisse, Jean-Stéphane |
author_facet |
Ben Amira, Maroua Mom, Robin Lopez, David Chaar, Hatem Khouaja, Ali Pujade-Renaud, Valérie Fumanal, Boris Gousset-Dupont, Aurélie Bronner, Gisèle Label, Philippe Julien, Jean-Louis Triki, Ali Mohamed Auguin, Daniel Venisse, Jean-Stéphane |
author_sort |
Ben Amira, Maroua |
title |
MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees |
title_short |
MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees |
title_full |
MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees |
title_fullStr |
MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees |
title_full_unstemmed |
MIP diversity from Trichoderma: Structural considerations and transcriptional modulation during mycoparasitic association with Fusarium solani olive trees |
title_sort |
mip diversity from trichoderma: structural considerations and transcriptional modulation during mycoparasitic association with fusarium solani olive trees |
url |
http://agritrop.cirad.fr/587687/ http://agritrop.cirad.fr/587687/1/journal.pone.0193760.pdf |
work_keys_str_mv |
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