Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system
The occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm), cesium (Cs+) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs+ from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs+ content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side-effects. Conversely, the development of biotechnological approaches aimed at reducing Cs+ accumulation in rice remain challenging. Here, we show that inactivation of the Cs+-permeable K+ transporter OsHAK1 with the CRISPR-Cas system dramatically reduced Cs+ uptake by rice plants. Cs+ uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs+ uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs+ and K+; and (ii) a high capacity to transport Cs+ from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with 137Cs+, plants lacking OsHAK1 function displayed strikingly reduced levels of 137Cs+ in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents.
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Subjects: | P02 - Pollution, Q03 - Contamination et toxicologie alimentaires, F60 - Physiologie et biochimie végétale, F01 - Culture des plantes, riz, Oryza sativa, contamination radioactive, césium, absorption, sol pollué, physiologie végétale, http://aims.fao.org/aos/agrovoc/c_6599, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_28321, http://aims.fao.org/aos/agrovoc/c_1182, http://aims.fao.org/aos/agrovoc/c_27, http://aims.fao.org/aos/agrovoc/c_34324, http://aims.fao.org/aos/agrovoc/c_25189, http://aims.fao.org/aos/agrovoc/c_4039, |
Online Access: | http://agritrop.cirad.fr/586754/ http://agritrop.cirad.fr/586754/1/Nieves-Cordones_et_al-2017-The_Plant_Journal.pdf |
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dig-cirad-fr-5867542024-01-29T00:45:49Z http://agritrop.cirad.fr/586754/ http://agritrop.cirad.fr/586754/ Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system. Nieves-Cordones Manuel, Mohamed Sonia, Tanoi Keitaro, Kobayashi Natsuko I., Takagi Keiko, Vernet Aurore, Guiderdoni Emmanuel, Périn Christophe, Sentenac Hervé, Very Anne-Aliénor. 2017. Plant Journal, 92 (1) : 43-56.https://doi.org/10.1111/tpj.13632 <https://doi.org/10.1111/tpj.13632> Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system Nieves-Cordones, Manuel Mohamed, Sonia Tanoi, Keitaro Kobayashi, Natsuko I. Takagi, Keiko Vernet, Aurore Guiderdoni, Emmanuel Périn, Christophe Sentenac, Hervé Very, Anne-Aliénor eng 2017 Plant Journal P02 - Pollution Q03 - Contamination et toxicologie alimentaires F60 - Physiologie et biochimie végétale F01 - Culture des plantes riz Oryza sativa contamination radioactive césium absorption sol pollué physiologie végétale http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_28321 http://aims.fao.org/aos/agrovoc/c_1182 http://aims.fao.org/aos/agrovoc/c_27 http://aims.fao.org/aos/agrovoc/c_34324 http://aims.fao.org/aos/agrovoc/c_25189 Japon http://aims.fao.org/aos/agrovoc/c_4039 The occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm), cesium (Cs+) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs+ from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs+ content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side-effects. Conversely, the development of biotechnological approaches aimed at reducing Cs+ accumulation in rice remain challenging. Here, we show that inactivation of the Cs+-permeable K+ transporter OsHAK1 with the CRISPR-Cas system dramatically reduced Cs+ uptake by rice plants. Cs+ uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs+ uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs+ and K+; and (ii) a high capacity to transport Cs+ from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with 137Cs+, plants lacking OsHAK1 function displayed strikingly reduced levels of 137Cs+ in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/586754/1/Nieves-Cordones_et_al-2017-The_Plant_Journal.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/tpj.13632 10.1111/tpj.13632 info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.13632 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/tpj.13632 |
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P02 - Pollution Q03 - Contamination et toxicologie alimentaires F60 - Physiologie et biochimie végétale F01 - Culture des plantes riz Oryza sativa contamination radioactive césium absorption sol pollué physiologie végétale http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_28321 http://aims.fao.org/aos/agrovoc/c_1182 http://aims.fao.org/aos/agrovoc/c_27 http://aims.fao.org/aos/agrovoc/c_34324 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_4039 P02 - Pollution Q03 - Contamination et toxicologie alimentaires F60 - Physiologie et biochimie végétale F01 - Culture des plantes riz Oryza sativa contamination radioactive césium absorption sol pollué physiologie végétale http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_28321 http://aims.fao.org/aos/agrovoc/c_1182 http://aims.fao.org/aos/agrovoc/c_27 http://aims.fao.org/aos/agrovoc/c_34324 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_4039 |
spellingShingle |
P02 - Pollution Q03 - Contamination et toxicologie alimentaires F60 - Physiologie et biochimie végétale F01 - Culture des plantes riz Oryza sativa contamination radioactive césium absorption sol pollué physiologie végétale http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_28321 http://aims.fao.org/aos/agrovoc/c_1182 http://aims.fao.org/aos/agrovoc/c_27 http://aims.fao.org/aos/agrovoc/c_34324 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_4039 P02 - Pollution Q03 - Contamination et toxicologie alimentaires F60 - Physiologie et biochimie végétale F01 - Culture des plantes riz Oryza sativa contamination radioactive césium absorption sol pollué physiologie végétale http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_28321 http://aims.fao.org/aos/agrovoc/c_1182 http://aims.fao.org/aos/agrovoc/c_27 http://aims.fao.org/aos/agrovoc/c_34324 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_4039 Nieves-Cordones, Manuel Mohamed, Sonia Tanoi, Keitaro Kobayashi, Natsuko I. Takagi, Keiko Vernet, Aurore Guiderdoni, Emmanuel Périn, Christophe Sentenac, Hervé Very, Anne-Aliénor Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system |
description |
The occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm), cesium (Cs+) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs+ from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs+ content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side-effects. Conversely, the development of biotechnological approaches aimed at reducing Cs+ accumulation in rice remain challenging. Here, we show that inactivation of the Cs+-permeable K+ transporter OsHAK1 with the CRISPR-Cas system dramatically reduced Cs+ uptake by rice plants. Cs+ uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs+ uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs+ and K+; and (ii) a high capacity to transport Cs+ from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with 137Cs+, plants lacking OsHAK1 function displayed strikingly reduced levels of 137Cs+ in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents. |
format |
article |
topic_facet |
P02 - Pollution Q03 - Contamination et toxicologie alimentaires F60 - Physiologie et biochimie végétale F01 - Culture des plantes riz Oryza sativa contamination radioactive césium absorption sol pollué physiologie végétale http://aims.fao.org/aos/agrovoc/c_6599 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_28321 http://aims.fao.org/aos/agrovoc/c_1182 http://aims.fao.org/aos/agrovoc/c_27 http://aims.fao.org/aos/agrovoc/c_34324 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_4039 |
author |
Nieves-Cordones, Manuel Mohamed, Sonia Tanoi, Keitaro Kobayashi, Natsuko I. Takagi, Keiko Vernet, Aurore Guiderdoni, Emmanuel Périn, Christophe Sentenac, Hervé Very, Anne-Aliénor |
author_facet |
Nieves-Cordones, Manuel Mohamed, Sonia Tanoi, Keitaro Kobayashi, Natsuko I. Takagi, Keiko Vernet, Aurore Guiderdoni, Emmanuel Périn, Christophe Sentenac, Hervé Very, Anne-Aliénor |
author_sort |
Nieves-Cordones, Manuel |
title |
Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system |
title_short |
Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system |
title_full |
Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system |
title_fullStr |
Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system |
title_full_unstemmed |
Production of low-Cs+ rice plants by inactivation of the K+ transporter OsHAK1 with the CRISPR-Cas system |
title_sort |
production of low-cs+ rice plants by inactivation of the k+ transporter oshak1 with the crispr-cas system |
url |
http://agritrop.cirad.fr/586754/ http://agritrop.cirad.fr/586754/1/Nieves-Cordones_et_al-2017-The_Plant_Journal.pdf |
work_keys_str_mv |
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