Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon
Background: The measure of new drug- or vaccine-based approaches for malaria control is based on direct membrane feeding assays (DMFAs) where gametocyte-infected blood samples are offered to mosquitoes through an artificial feeder system. Gametocyte donors are identified by the microscopic detection and quantification of malaria blood stages on blood films prepared using either capillary or venous blood. However, parasites are known to sequester in the microvasculature and this phenomenon may alter accurate detection of parasites in blood films. The blood source may then impact the success of mosquito feeding experiments and investigations are needed for the implementation of DMFAs under natural conditions. Methods: Thick blood smears were prepared from blood obtained from asymptomatic children attending primary schools in the vicinity of Mfou (Cameroon) over four transmission seasons. Parasite densities were determined microscopically from capillary and venous blood for 137 naturally-infected gametocyte carriers. The effect of the blood source on gametocyte and asexual stage densities was then assessed by fitting cumulative link mixed models (CLMM). DMFAs were performed to compare the infectiousness of gametocytes from the different blood sources to mosquitoes. Results: Prevalence of Plasmodium falciparum asexual stages among asymptomatic children aged from 4 to 15 years was 51.8% (2116/4087). The overall prevalence of P. falciparum gametocyte carriage was 8.9% and varied from one school to another. No difference in the density of gametocyte and asexual stages was found between capillary and venous blood. Attempts to perform DMFAs with capillary blood failed. Conclusions: Plasmodium falciparum malaria parasite densities do not differ between capillary and venous blood in asymptomatic subjects for both gametocyte and trophozoite stages. This finding suggests that the blood source should not interfere with transmission efficiency in DMFAs.
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Subjects: | L73 - Maladies des animaux, 000 - Autres thèmes, L72 - Organismes nuisibles des animaux, Plasmodium falciparum, malaria, sang, enfant, infection, épidémiologie, transmission des maladies, parasitisme, http://aims.fao.org/aos/agrovoc/c_31217, http://aims.fao.org/aos/agrovoc/c_34312, http://aims.fao.org/aos/agrovoc/c_959, http://aims.fao.org/aos/agrovoc/c_1547, http://aims.fao.org/aos/agrovoc/c_3852, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_2329, http://aims.fao.org/aos/agrovoc/c_5577, http://aims.fao.org/aos/agrovoc/c_1229, |
Online Access: | http://agritrop.cirad.fr/586712/ http://agritrop.cirad.fr/586712/1/Sandeu_2017.pdf |
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dig-cirad-fr-5867122024-01-29T00:45:02Z http://agritrop.cirad.fr/586712/ http://agritrop.cirad.fr/586712/ Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon. Sandeu Maurice M., Bayibéki Albert N., Tchioffo Majoline T., Abate Luc, Gimonneau Geoffrey, Awono-Ambéné Parfait, Nsango Sandrine E., Diallo Diadier, Berry Antoine, Texier Gaëtan, Morlais Isabelle. 2017. Malaria Journal, 16:345, 9 p.https://doi.org/10.1186/s12936-017-1978-6 <https://doi.org/10.1186/s12936-017-1978-6> Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon Sandeu, Maurice M. Bayibéki, Albert N. Tchioffo, Majoline T. Abate, Luc Gimonneau, Geoffrey Awono-Ambéné, Parfait Nsango, Sandrine E. Diallo, Diadier Berry, Antoine Texier, Gaëtan Morlais, Isabelle eng 2017 Malaria Journal L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Plasmodium falciparum malaria sang enfant infection épidémiologie transmission des maladies parasitisme http://aims.fao.org/aos/agrovoc/c_31217 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_1547 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5577 Cameroun http://aims.fao.org/aos/agrovoc/c_1229 Background: The measure of new drug- or vaccine-based approaches for malaria control is based on direct membrane feeding assays (DMFAs) where gametocyte-infected blood samples are offered to mosquitoes through an artificial feeder system. Gametocyte donors are identified by the microscopic detection and quantification of malaria blood stages on blood films prepared using either capillary or venous blood. However, parasites are known to sequester in the microvasculature and this phenomenon may alter accurate detection of parasites in blood films. The blood source may then impact the success of mosquito feeding experiments and investigations are needed for the implementation of DMFAs under natural conditions. Methods: Thick blood smears were prepared from blood obtained from asymptomatic children attending primary schools in the vicinity of Mfou (Cameroon) over four transmission seasons. Parasite densities were determined microscopically from capillary and venous blood for 137 naturally-infected gametocyte carriers. The effect of the blood source on gametocyte and asexual stage densities was then assessed by fitting cumulative link mixed models (CLMM). DMFAs were performed to compare the infectiousness of gametocytes from the different blood sources to mosquitoes. Results: Prevalence of Plasmodium falciparum asexual stages among asymptomatic children aged from 4 to 15 years was 51.8% (2116/4087). The overall prevalence of P. falciparum gametocyte carriage was 8.9% and varied from one school to another. No difference in the density of gametocyte and asexual stages was found between capillary and venous blood. Attempts to perform DMFAs with capillary blood failed. Conclusions: Plasmodium falciparum malaria parasite densities do not differ between capillary and venous blood in asymptomatic subjects for both gametocyte and trophozoite stages. This finding suggests that the blood source should not interfere with transmission efficiency in DMFAs. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/586712/1/Sandeu_2017.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/s12936-017-1978-6 10.1186/s12936-017-1978-6 info:eu-repo/semantics/altIdentifier/doi/10.1186/s12936-017-1978-6 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/s12936-017-1978-6 |
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L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Plasmodium falciparum malaria sang enfant infection épidémiologie transmission des maladies parasitisme http://aims.fao.org/aos/agrovoc/c_31217 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_1547 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5577 http://aims.fao.org/aos/agrovoc/c_1229 L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Plasmodium falciparum malaria sang enfant infection épidémiologie transmission des maladies parasitisme http://aims.fao.org/aos/agrovoc/c_31217 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_1547 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5577 http://aims.fao.org/aos/agrovoc/c_1229 |
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L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Plasmodium falciparum malaria sang enfant infection épidémiologie transmission des maladies parasitisme http://aims.fao.org/aos/agrovoc/c_31217 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_1547 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5577 http://aims.fao.org/aos/agrovoc/c_1229 L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Plasmodium falciparum malaria sang enfant infection épidémiologie transmission des maladies parasitisme http://aims.fao.org/aos/agrovoc/c_31217 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_1547 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5577 http://aims.fao.org/aos/agrovoc/c_1229 Sandeu, Maurice M. Bayibéki, Albert N. Tchioffo, Majoline T. Abate, Luc Gimonneau, Geoffrey Awono-Ambéné, Parfait Nsango, Sandrine E. Diallo, Diadier Berry, Antoine Texier, Gaëtan Morlais, Isabelle Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon |
description |
Background: The measure of new drug- or vaccine-based approaches for malaria control is based on direct membrane feeding assays (DMFAs) where gametocyte-infected blood samples are offered to mosquitoes through an artificial feeder system. Gametocyte donors are identified by the microscopic detection and quantification of malaria blood stages on blood films prepared using either capillary or venous blood. However, parasites are known to sequester in the microvasculature and this phenomenon may alter accurate detection of parasites in blood films. The blood source may then impact the success of mosquito feeding experiments and investigations are needed for the implementation of DMFAs under natural conditions. Methods: Thick blood smears were prepared from blood obtained from asymptomatic children attending primary schools in the vicinity of Mfou (Cameroon) over four transmission seasons. Parasite densities were determined microscopically from capillary and venous blood for 137 naturally-infected gametocyte carriers. The effect of the blood source on gametocyte and asexual stage densities was then assessed by fitting cumulative link mixed models (CLMM). DMFAs were performed to compare the infectiousness of gametocytes from the different blood sources to mosquitoes. Results: Prevalence of Plasmodium falciparum asexual stages among asymptomatic children aged from 4 to 15 years was 51.8% (2116/4087). The overall prevalence of P. falciparum gametocyte carriage was 8.9% and varied from one school to another. No difference in the density of gametocyte and asexual stages was found between capillary and venous blood. Attempts to perform DMFAs with capillary blood failed. Conclusions: Plasmodium falciparum malaria parasite densities do not differ between capillary and venous blood in asymptomatic subjects for both gametocyte and trophozoite stages. This finding suggests that the blood source should not interfere with transmission efficiency in DMFAs. |
format |
article |
topic_facet |
L73 - Maladies des animaux 000 - Autres thèmes L72 - Organismes nuisibles des animaux Plasmodium falciparum malaria sang enfant infection épidémiologie transmission des maladies parasitisme http://aims.fao.org/aos/agrovoc/c_31217 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_1547 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_5577 http://aims.fao.org/aos/agrovoc/c_1229 |
author |
Sandeu, Maurice M. Bayibéki, Albert N. Tchioffo, Majoline T. Abate, Luc Gimonneau, Geoffrey Awono-Ambéné, Parfait Nsango, Sandrine E. Diallo, Diadier Berry, Antoine Texier, Gaëtan Morlais, Isabelle |
author_facet |
Sandeu, Maurice M. Bayibéki, Albert N. Tchioffo, Majoline T. Abate, Luc Gimonneau, Geoffrey Awono-Ambéné, Parfait Nsango, Sandrine E. Diallo, Diadier Berry, Antoine Texier, Gaëtan Morlais, Isabelle |
author_sort |
Sandeu, Maurice M. |
title |
Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon |
title_short |
Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon |
title_full |
Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon |
title_fullStr |
Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon |
title_full_unstemmed |
Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon |
title_sort |
do the venous blood samples replicate malaria parasite densities found in capillary blood? a field study performed in naturally-infected asymptomatic children in cameroon |
url |
http://agritrop.cirad.fr/586712/ http://agritrop.cirad.fr/586712/1/Sandeu_2017.pdf |
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