Detection and distribution of bovine trypanosomiasis in Malawi
Polymerase chain reaction-restriction length polymorphism (PCR-RFLP) was used to identify trypanosome species in cattle from the three regions of Malawi. A total of 444 DNA samples were screened for trypanosomes using PCR-RFLP performed on whole blood samples collected from cattle between January 2016 and February 2016. A questionnaire was administered to the farmers of sampled cattle that contained sections on demographics and livestock management. Due to its zoonotic importance, T.brucei status was chosen to compare data among surveyed farms and Mann-Whitney U tests were employed for these comparisons. Prevalence information and collected questionnaire data were analysed using OpenEpi. Out of the 440 cattle with DNA samples, 2% (n=9; 95% CI: 1-3) were positive for Trypanosoma brucei, 3% (n=14; 95% CI: 1-5) were positive for Trypanosoma congolense and 27% (n=120; 95% CI: 23-31) were positive for Trypanosoma theileri. T.theileri and T.congolense appeared randomly distributed across the country while T.brucei was restricted to the central and southern regions of Malawi. The majority of the respondents were farmers (92%; 95% CI: 82-97) that were literate with the median education level being grade 7. Most respondents were smallholder farmers with a median herd size of 7 cattle and owning 1.2 hectares of land. There were no differences between the T.brucei positive and T.brucei negative groups in respect to education level (p=0.340), cattle owned (p=0.449) and land owned (p=0.920). (Texte intégral)
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dig-cirad-fr-5867062021-01-04T12:15:44Z http://agritrop.cirad.fr/586706/ http://agritrop.cirad.fr/586706/ Detection and distribution of bovine trypanosomiasis in Malawi. Chimera Elizabeth T., Fosgate Geoffrey T., Neves L., Etter Eric. 2017. In : Proceedings of the 9th Veterinary, ParaVeterinary and SASVEPM congress 24-27 July 2017. Lobetti Remo (ed.). s.l. : SAVA-SASVEPM, Résumé, 324. 9th Veterinary, Paraveterinary and SASVEPM congress2017, Boksburg, Afrique du Sud, 24 Juillet 2017/27 Juillet 2017. Researchers Detection and distribution of bovine trypanosomiasis in Malawi Chimera, Elizabeth T. Fosgate, Geoffrey T. Neves, L. Etter, Eric eng 2017 SAVA Proceedings of the 9th Veterinary, ParaVeterinary and SASVEPM congress 24-27 July 2017 L73 - Maladies des animaux U30 - Méthodes de recherche Polymerase chain reaction-restriction length polymorphism (PCR-RFLP) was used to identify trypanosome species in cattle from the three regions of Malawi. A total of 444 DNA samples were screened for trypanosomes using PCR-RFLP performed on whole blood samples collected from cattle between January 2016 and February 2016. A questionnaire was administered to the farmers of sampled cattle that contained sections on demographics and livestock management. Due to its zoonotic importance, T.brucei status was chosen to compare data among surveyed farms and Mann-Whitney U tests were employed for these comparisons. Prevalence information and collected questionnaire data were analysed using OpenEpi. Out of the 440 cattle with DNA samples, 2% (n=9; 95% CI: 1-3) were positive for Trypanosoma brucei, 3% (n=14; 95% CI: 1-5) were positive for Trypanosoma congolense and 27% (n=120; 95% CI: 23-31) were positive for Trypanosoma theileri. T.theileri and T.congolense appeared randomly distributed across the country while T.brucei was restricted to the central and southern regions of Malawi. The majority of the respondents were farmers (92%; 95% CI: 82-97) that were literate with the median education level being grade 7. Most respondents were smallholder farmers with a median herd size of 7 cattle and owning 1.2 hectares of land. There were no differences between the T.brucei positive and T.brucei negative groups in respect to education level (p=0.340), cattle owned (p=0.449) and land owned (p=0.920). (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/586706/7/ID586706.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html |
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L73 - Maladies des animaux U30 - Méthodes de recherche L73 - Maladies des animaux U30 - Méthodes de recherche Chimera, Elizabeth T. Fosgate, Geoffrey T. Neves, L. Etter, Eric Detection and distribution of bovine trypanosomiasis in Malawi |
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Polymerase chain reaction-restriction length polymorphism (PCR-RFLP) was used to identify trypanosome species in cattle from the three regions of Malawi. A total of 444 DNA samples were screened for trypanosomes using PCR-RFLP performed on whole blood samples collected from cattle between January 2016 and February 2016. A questionnaire was administered to the farmers of sampled cattle that contained sections on demographics and livestock management. Due to its zoonotic importance, T.brucei status was chosen to compare data among surveyed farms and Mann-Whitney U tests were employed for these comparisons. Prevalence information and collected questionnaire data were analysed using OpenEpi. Out of the 440 cattle with DNA samples, 2% (n=9; 95% CI: 1-3) were positive for Trypanosoma brucei, 3% (n=14; 95% CI: 1-5) were positive for Trypanosoma congolense and 27% (n=120; 95% CI: 23-31) were positive for Trypanosoma theileri. T.theileri and T.congolense appeared randomly distributed across the country while T.brucei was restricted to the central and southern regions of Malawi. The majority of the respondents were farmers (92%; 95% CI: 82-97) that were literate with the median education level being grade 7. Most respondents were smallholder farmers with a median herd size of 7 cattle and owning 1.2 hectares of land. There were no differences between the T.brucei positive and T.brucei negative groups in respect to education level (p=0.340), cattle owned (p=0.449) and land owned (p=0.920). (Texte intégral) |
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conference_item |
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L73 - Maladies des animaux U30 - Méthodes de recherche |
author |
Chimera, Elizabeth T. Fosgate, Geoffrey T. Neves, L. Etter, Eric |
author_facet |
Chimera, Elizabeth T. Fosgate, Geoffrey T. Neves, L. Etter, Eric |
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Chimera, Elizabeth T. |
title |
Detection and distribution of bovine trypanosomiasis in Malawi |
title_short |
Detection and distribution of bovine trypanosomiasis in Malawi |
title_full |
Detection and distribution of bovine trypanosomiasis in Malawi |
title_fullStr |
Detection and distribution of bovine trypanosomiasis in Malawi |
title_full_unstemmed |
Detection and distribution of bovine trypanosomiasis in Malawi |
title_sort |
detection and distribution of bovine trypanosomiasis in malawi |
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SAVA |
url |
http://agritrop.cirad.fr/586706/ http://agritrop.cirad.fr/586706/7/ID586706.pdf |
work_keys_str_mv |
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