Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple
Fusarium ananatum causes fruitlet core rot (FCR) in pineapple (Ananas comosus var. comosus) when the fruit reaches maturity. Hidden symptoms make it difficult to assess the disease, regardless of its stage, and basic questions concerning the involvement of the phenolic compounds in response to infection remain unknown. A direct inoculation method of F. ananatum in pineapple fruitlets was developed to monitor the growth of black spots and the changes in phenolic acids and ascorbic acid concentration under controlled conditions. After inoculation, infection began with a flesh discolouration at the inoculation point and then spread in a darker shade to form a black spot. Coumaroyl-isocitric and caffeoyl-isocitric acids levels respectively showed a 150- and 200-fold increase in infected fruitlet when compared to healthy fruitlet. These hydroxycinnamic acids increased minimally in the adjacent fruitlet and remained stable in the other parts of the fruit. By contrast, sinapic acid and hydroxybenzoic acid isomers (HBA) decreased after F. ananatum inoculation in the infected fruitlet, whereas they remained stable in the adjacent and healthy fruitlets. Ascorbic acid decreased to zero in the infected fruitlet. The antifungal activity of phenolic compounds and ascorbic acid was evaluated against the mycelial growth of F. ananatum. p-Coumaric acid exhibited a total inhibition of the mycelial growth at 1000 μg g−1. Ferulic acid inhibited 64 % of mycelial growth at a concentration of 1000 μg g−1. Caffeoylquinic acid, sinapic acid, and ascorbic acid also showed significant antifungal activity, but to a lesser extent. Finally, coinoculation of the hydroxycinnamic acids with the pathogen restrains its development in the fruit. This is the first study to highlight the involvement of phenolic compounds in the pineapple FCR disease.
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Subjects: | H20 - Maladies des plantes, Ananas comosus, composé phénolique, acide ascorbique, Fusarium, Ananas (genre), pourriture, croissance, http://aims.fao.org/aos/agrovoc/c_386, http://aims.fao.org/aos/agrovoc/c_5772, http://aims.fao.org/aos/agrovoc/c_661, http://aims.fao.org/aos/agrovoc/c_3156, http://aims.fao.org/aos/agrovoc/c_385, http://aims.fao.org/aos/agrovoc/c_6667, http://aims.fao.org/aos/agrovoc/c_3394, http://aims.fao.org/aos/agrovoc/c_3081, |
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dig-cirad-fr-5862032024-01-29T05:41:25Z http://agritrop.cirad.fr/586203/ http://agritrop.cirad.fr/586203/ Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple. Barral Bastien, Chillet Marc, Minier Jérôme, Lechaudel Mathieu, Schorr-Galindo Sabine. 2017. Fungal Biology, 121 (12) : 1045-1053.https://doi.org/10.1016/j.funbio.2017.09.002 <https://doi.org/10.1016/j.funbio.2017.09.002> Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple Barral, Bastien Chillet, Marc Minier, Jérôme Lechaudel, Mathieu Schorr-Galindo, Sabine eng 2017 Fungal Biology H20 - Maladies des plantes Ananas comosus composé phénolique acide ascorbique Fusarium Ananas (genre) pourriture croissance http://aims.fao.org/aos/agrovoc/c_386 http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_661 http://aims.fao.org/aos/agrovoc/c_3156 http://aims.fao.org/aos/agrovoc/c_385 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_3394 France http://aims.fao.org/aos/agrovoc/c_3081 Fusarium ananatum causes fruitlet core rot (FCR) in pineapple (Ananas comosus var. comosus) when the fruit reaches maturity. Hidden symptoms make it difficult to assess the disease, regardless of its stage, and basic questions concerning the involvement of the phenolic compounds in response to infection remain unknown. A direct inoculation method of F. ananatum in pineapple fruitlets was developed to monitor the growth of black spots and the changes in phenolic acids and ascorbic acid concentration under controlled conditions. After inoculation, infection began with a flesh discolouration at the inoculation point and then spread in a darker shade to form a black spot. Coumaroyl-isocitric and caffeoyl-isocitric acids levels respectively showed a 150- and 200-fold increase in infected fruitlet when compared to healthy fruitlet. These hydroxycinnamic acids increased minimally in the adjacent fruitlet and remained stable in the other parts of the fruit. By contrast, sinapic acid and hydroxybenzoic acid isomers (HBA) decreased after F. ananatum inoculation in the infected fruitlet, whereas they remained stable in the adjacent and healthy fruitlets. Ascorbic acid decreased to zero in the infected fruitlet. The antifungal activity of phenolic compounds and ascorbic acid was evaluated against the mycelial growth of F. ananatum. p-Coumaric acid exhibited a total inhibition of the mycelial growth at 1000 μg g−1. Ferulic acid inhibited 64 % of mycelial growth at a concentration of 1000 μg g−1. Caffeoylquinic acid, sinapic acid, and ascorbic acid also showed significant antifungal activity, but to a lesser extent. Finally, coinoculation of the hydroxycinnamic acids with the pathogen restrains its development in the fruit. This is the first study to highlight the involvement of phenolic compounds in the pineapple FCR disease. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/586203/1/1-s2.0-S1878614617301253-main.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.funbio.2017.09.002 10.1016/j.funbio.2017.09.002 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.funbio.2017.09.002 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.funbio.2017.09.002 |
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H20 - Maladies des plantes Ananas comosus composé phénolique acide ascorbique Fusarium Ananas (genre) pourriture croissance http://aims.fao.org/aos/agrovoc/c_386 http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_661 http://aims.fao.org/aos/agrovoc/c_3156 http://aims.fao.org/aos/agrovoc/c_385 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Ananas comosus composé phénolique acide ascorbique Fusarium Ananas (genre) pourriture croissance http://aims.fao.org/aos/agrovoc/c_386 http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_661 http://aims.fao.org/aos/agrovoc/c_3156 http://aims.fao.org/aos/agrovoc/c_385 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
H20 - Maladies des plantes Ananas comosus composé phénolique acide ascorbique Fusarium Ananas (genre) pourriture croissance http://aims.fao.org/aos/agrovoc/c_386 http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_661 http://aims.fao.org/aos/agrovoc/c_3156 http://aims.fao.org/aos/agrovoc/c_385 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Ananas comosus composé phénolique acide ascorbique Fusarium Ananas (genre) pourriture croissance http://aims.fao.org/aos/agrovoc/c_386 http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_661 http://aims.fao.org/aos/agrovoc/c_3156 http://aims.fao.org/aos/agrovoc/c_385 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_3081 Barral, Bastien Chillet, Marc Minier, Jérôme Lechaudel, Mathieu Schorr-Galindo, Sabine Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple |
description |
Fusarium ananatum causes fruitlet core rot (FCR) in pineapple (Ananas comosus var. comosus) when the fruit reaches maturity. Hidden symptoms make it difficult to assess the disease, regardless of its stage, and basic questions concerning the involvement of the phenolic compounds in response to infection remain unknown. A direct inoculation method of F. ananatum in pineapple fruitlets was developed to monitor the growth of black spots and the changes in phenolic acids and ascorbic acid concentration under controlled conditions. After inoculation, infection began with a flesh discolouration at the inoculation point and then spread in a darker shade to form a black spot. Coumaroyl-isocitric and caffeoyl-isocitric acids levels respectively showed a 150- and 200-fold increase in infected fruitlet when compared to healthy fruitlet. These hydroxycinnamic acids increased minimally in the adjacent fruitlet and remained stable in the other parts of the fruit. By contrast, sinapic acid and hydroxybenzoic acid isomers (HBA) decreased after F. ananatum inoculation in the infected fruitlet, whereas they remained stable in the adjacent and healthy fruitlets. Ascorbic acid decreased to zero in the infected fruitlet. The antifungal activity of phenolic compounds and ascorbic acid was evaluated against the mycelial growth of F. ananatum. p-Coumaric acid exhibited a total inhibition of the mycelial growth at 1000 μg g−1. Ferulic acid inhibited 64 % of mycelial growth at a concentration of 1000 μg g−1. Caffeoylquinic acid, sinapic acid, and ascorbic acid also showed significant antifungal activity, but to a lesser extent. Finally, coinoculation of the hydroxycinnamic acids with the pathogen restrains its development in the fruit. This is the first study to highlight the involvement of phenolic compounds in the pineapple FCR disease. |
format |
article |
topic_facet |
H20 - Maladies des plantes Ananas comosus composé phénolique acide ascorbique Fusarium Ananas (genre) pourriture croissance http://aims.fao.org/aos/agrovoc/c_386 http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_661 http://aims.fao.org/aos/agrovoc/c_3156 http://aims.fao.org/aos/agrovoc/c_385 http://aims.fao.org/aos/agrovoc/c_6667 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Barral, Bastien Chillet, Marc Minier, Jérôme Lechaudel, Mathieu Schorr-Galindo, Sabine |
author_facet |
Barral, Bastien Chillet, Marc Minier, Jérôme Lechaudel, Mathieu Schorr-Galindo, Sabine |
author_sort |
Barral, Bastien |
title |
Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple |
title_short |
Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple |
title_full |
Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple |
title_fullStr |
Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple |
title_full_unstemmed |
Evaluating the response to Fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple |
title_sort |
evaluating the response to fusarium ananatum inoculation and antifungal activity of phenolic acids in pineapple |
url |
http://agritrop.cirad.fr/586203/ http://agritrop.cirad.fr/586203/1/1-s2.0-S1878614617301253-main.pdf |
work_keys_str_mv |
AT barralbastien evaluatingtheresponsetofusariumananatuminoculationandantifungalactivityofphenolicacidsinpineapple AT chilletmarc evaluatingtheresponsetofusariumananatuminoculationandantifungalactivityofphenolicacidsinpineapple AT minierjerome evaluatingtheresponsetofusariumananatuminoculationandantifungalactivityofphenolicacidsinpineapple AT lechaudelmathieu evaluatingtheresponsetofusariumananatuminoculationandantifungalactivityofphenolicacidsinpineapple AT schorrgalindosabine evaluatingtheresponsetofusariumananatuminoculationandantifungalactivityofphenolicacidsinpineapple |
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1792499390465703936 |