Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae)
The variegated coffee bug Antestiopsis thunbergii Gmelin is a key pest of Coffea arabica in East Africa. Although the bug feeds on various parts of the coffee plant, it has a strong preference for mature green berries which are essential for the bug to complete its life cycle, reproduce and enhance its longevity. To locate mature green coffee berries, we hypothesized that second instar nymphs, which are the most mobile and active feeding immature stage of the bug, must rely on key volatile compounds emitted by the host for recognition. We tested this hypothesis using behavioral and electrophysiological assays and chemical analysis. In olfactometer assays, the second instar nymphs were strongly attracted to volatiles emitted from mature green berries but avoided those from ripe coffee berries of C. arabica. Coupled gas chromatography–electroantennographic detection (GC/EAD) isolated five antenally active components from mature green berries volatiles, four of which were identified by coupled GC–mass spectrometry as toluene, anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin. In concentration assays, in which second instar nymphs did not respond to toluene, they were strongly attracted to anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin, and a blend from the three compounds at concentrations lower or equivalent to the natural volatile extract. Our results suggest that the blend from these three compounds allows host recognition in second instar nymphs of the variegated coffee bug and is a candidate kairomone for monitoring the pest in coffee plantations.
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Subjects: | H10 - Ravageurs des plantes, U30 - Méthodes de recherche, Coffea arabica, kairomones, Pentatomidae, relation hôte parasite, composé volatil, composé de la flaveur, attractif, maturité, chromatographie en phase gazeuse, spectrométrie de masse, http://aims.fao.org/aos/agrovoc/c_1721, http://aims.fao.org/aos/agrovoc/c_32487, http://aims.fao.org/aos/agrovoc/c_30684, http://aims.fao.org/aos/agrovoc/c_11620, http://aims.fao.org/aos/agrovoc/c_24933, http://aims.fao.org/aos/agrovoc/c_28309, http://aims.fao.org/aos/agrovoc/c_705, http://aims.fao.org/aos/agrovoc/c_4656, http://aims.fao.org/aos/agrovoc/c_11097, http://aims.fao.org/aos/agrovoc/c_12421, http://aims.fao.org/aos/agrovoc/c_4086, |
Online Access: | http://agritrop.cirad.fr/585934/ http://agritrop.cirad.fr/585934/1/Nji4.pdf |
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dig-cirad-fr-5859342024-01-29T00:38:08Z http://agritrop.cirad.fr/585934/ http://agritrop.cirad.fr/585934/ Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae). Njihia Teresiah, Torto Baldwin, Murungi Lucy Kananu, Irungu Janet, Mwenda Dickson, Babin Régis. 2017. Chemoecology, 27 (6) : 239-248.https://doi.org/10.1007/s00049-017-0248-y <https://doi.org/10.1007/s00049-017-0248-y> Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae) Njihia, Teresiah Torto, Baldwin Murungi, Lucy Kananu Irungu, Janet Mwenda, Dickson Babin, Régis eng 2017 Chemoecology H10 - Ravageurs des plantes U30 - Méthodes de recherche Coffea arabica kairomones Pentatomidae relation hôte parasite composé volatil composé de la flaveur attractif maturité chromatographie en phase gazeuse spectrométrie de masse http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32487 http://aims.fao.org/aos/agrovoc/c_30684 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_24933 http://aims.fao.org/aos/agrovoc/c_28309 http://aims.fao.org/aos/agrovoc/c_705 http://aims.fao.org/aos/agrovoc/c_4656 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_12421 Kenya http://aims.fao.org/aos/agrovoc/c_4086 The variegated coffee bug Antestiopsis thunbergii Gmelin is a key pest of Coffea arabica in East Africa. Although the bug feeds on various parts of the coffee plant, it has a strong preference for mature green berries which are essential for the bug to complete its life cycle, reproduce and enhance its longevity. To locate mature green coffee berries, we hypothesized that second instar nymphs, which are the most mobile and active feeding immature stage of the bug, must rely on key volatile compounds emitted by the host for recognition. We tested this hypothesis using behavioral and electrophysiological assays and chemical analysis. In olfactometer assays, the second instar nymphs were strongly attracted to volatiles emitted from mature green berries but avoided those from ripe coffee berries of C. arabica. Coupled gas chromatography–electroantennographic detection (GC/EAD) isolated five antenally active components from mature green berries volatiles, four of which were identified by coupled GC–mass spectrometry as toluene, anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin. In concentration assays, in which second instar nymphs did not respond to toluene, they were strongly attracted to anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin, and a blend from the three compounds at concentrations lower or equivalent to the natural volatile extract. Our results suggest that the blend from these three compounds allows host recognition in second instar nymphs of the variegated coffee bug and is a candidate kairomone for monitoring the pest in coffee plantations. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/585934/1/Nji4.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00049-017-0248-y 10.1007/s00049-017-0248-y info:eu-repo/semantics/altIdentifier/doi/10.1007/s00049-017-0248-y info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00049-017-0248-y |
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H10 - Ravageurs des plantes U30 - Méthodes de recherche Coffea arabica kairomones Pentatomidae relation hôte parasite composé volatil composé de la flaveur attractif maturité chromatographie en phase gazeuse spectrométrie de masse http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32487 http://aims.fao.org/aos/agrovoc/c_30684 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_24933 http://aims.fao.org/aos/agrovoc/c_28309 http://aims.fao.org/aos/agrovoc/c_705 http://aims.fao.org/aos/agrovoc/c_4656 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4086 H10 - Ravageurs des plantes U30 - Méthodes de recherche Coffea arabica kairomones Pentatomidae relation hôte parasite composé volatil composé de la flaveur attractif maturité chromatographie en phase gazeuse spectrométrie de masse http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32487 http://aims.fao.org/aos/agrovoc/c_30684 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_24933 http://aims.fao.org/aos/agrovoc/c_28309 http://aims.fao.org/aos/agrovoc/c_705 http://aims.fao.org/aos/agrovoc/c_4656 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4086 |
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H10 - Ravageurs des plantes U30 - Méthodes de recherche Coffea arabica kairomones Pentatomidae relation hôte parasite composé volatil composé de la flaveur attractif maturité chromatographie en phase gazeuse spectrométrie de masse http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32487 http://aims.fao.org/aos/agrovoc/c_30684 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_24933 http://aims.fao.org/aos/agrovoc/c_28309 http://aims.fao.org/aos/agrovoc/c_705 http://aims.fao.org/aos/agrovoc/c_4656 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4086 H10 - Ravageurs des plantes U30 - Méthodes de recherche Coffea arabica kairomones Pentatomidae relation hôte parasite composé volatil composé de la flaveur attractif maturité chromatographie en phase gazeuse spectrométrie de masse http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32487 http://aims.fao.org/aos/agrovoc/c_30684 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_24933 http://aims.fao.org/aos/agrovoc/c_28309 http://aims.fao.org/aos/agrovoc/c_705 http://aims.fao.org/aos/agrovoc/c_4656 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4086 Njihia, Teresiah Torto, Baldwin Murungi, Lucy Kananu Irungu, Janet Mwenda, Dickson Babin, Régis Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae) |
description |
The variegated coffee bug Antestiopsis thunbergii Gmelin is a key pest of Coffea arabica in East Africa. Although the bug feeds on various parts of the coffee plant, it has a strong preference for mature green berries which are essential for the bug to complete its life cycle, reproduce and enhance its longevity. To locate mature green coffee berries, we hypothesized that second instar nymphs, which are the most mobile and active feeding immature stage of the bug, must rely on key volatile compounds emitted by the host for recognition. We tested this hypothesis using behavioral and electrophysiological assays and chemical analysis. In olfactometer assays, the second instar nymphs were strongly attracted to volatiles emitted from mature green berries but avoided those from ripe coffee berries of C. arabica. Coupled gas chromatography–electroantennographic detection (GC/EAD) isolated five antenally active components from mature green berries volatiles, four of which were identified by coupled GC–mass spectrometry as toluene, anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin. In concentration assays, in which second instar nymphs did not respond to toluene, they were strongly attracted to anisole, methyl 3-ethyl-4-methylpentanoate and (5S,7S)-conophthorin, and a blend from the three compounds at concentrations lower or equivalent to the natural volatile extract. Our results suggest that the blend from these three compounds allows host recognition in second instar nymphs of the variegated coffee bug and is a candidate kairomone for monitoring the pest in coffee plantations. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes U30 - Méthodes de recherche Coffea arabica kairomones Pentatomidae relation hôte parasite composé volatil composé de la flaveur attractif maturité chromatographie en phase gazeuse spectrométrie de masse http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32487 http://aims.fao.org/aos/agrovoc/c_30684 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_24933 http://aims.fao.org/aos/agrovoc/c_28309 http://aims.fao.org/aos/agrovoc/c_705 http://aims.fao.org/aos/agrovoc/c_4656 http://aims.fao.org/aos/agrovoc/c_11097 http://aims.fao.org/aos/agrovoc/c_12421 http://aims.fao.org/aos/agrovoc/c_4086 |
author |
Njihia, Teresiah Torto, Baldwin Murungi, Lucy Kananu Irungu, Janet Mwenda, Dickson Babin, Régis |
author_facet |
Njihia, Teresiah Torto, Baldwin Murungi, Lucy Kananu Irungu, Janet Mwenda, Dickson Babin, Régis |
author_sort |
Njihia, Teresiah |
title |
Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae) |
title_short |
Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae) |
title_full |
Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae) |
title_fullStr |
Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae) |
title_full_unstemmed |
Identification of kairomones of second instar nymphs of the variegated coffee bug Antestiopsis thunbergii (Heteroptera: Pentatomidae) |
title_sort |
identification of kairomones of second instar nymphs of the variegated coffee bug antestiopsis thunbergii (heteroptera: pentatomidae) |
url |
http://agritrop.cirad.fr/585934/ http://agritrop.cirad.fr/585934/1/Nji4.pdf |
work_keys_str_mv |
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_version_ |
1792499377255743488 |