Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs
Japanese encephalitis remains the most important cause of viral encephalitis in humans in several southeast Asian countries, including Cambodia, causing at least 65 000 cases of encephalitis per year. This vector-borne viral zoonosis – caused by Japanese encephalitis virus (JEV) – is considered to be a rural disease and is transmitted by mosquitoes, with birds and pigs being the natural reservoirs, while humans are accidental hosts. In this study we report the first two JEV isolations in Cambodia from human encephalitis cases from two studies on the aetiology of central nervous system disease, conducted at the two major paediatric hospitals in the country. We also report JEV isolation from Culextritaeniorhynchus mosquitoes and from pig samples collected in two farms, located in peri-urban and rural areas. Out of 11 reverse-transcription polymerase chain reaction-positive original samples, we generated full-genome sequences from 5 JEV isolates. Five additional partial sequences of the JEV NS3 gene from viruses detected in five pigs and one complete coding sequence of the envelope gene of a strain identified in a pig were generated. Phylogenetic analyses revealed that JEV detected in Cambodia belonged to genotype I and clustered in two clades: genotype I-a, mainly comprising strains from Thailand, and genotype I-b, comprising strains from Vietnam that dispersed northwards to China. Finally, in this study, we provide proof that the sequenced JEV strains circulate between pigs, Culex tritaeniorhynchus and humans in the Phnom Penh vicinity.
Main Authors: | , , , , , , , , , , , , , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | S50 - Santé humaine, L73 - Maladies des animaux, U30 - Méthodes de recherche, http://aims.fao.org/aos/agrovoc/c_4073, |
Online Access: | http://agritrop.cirad.fr/585281/ http://agritrop.cirad.fr/585281/7/2287_vir000892.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-585281 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5852812022-03-30T12:03:12Z http://agritrop.cirad.fr/585281/ http://agritrop.cirad.fr/585281/ Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs. Duong Veasna, Choeung Rithy, Gorman Christopher, Laurent Denis, Crabol Yoann, Mey Channa, Peng Borin, Di Francesco Juliette, Hul Vibol, Sothy Heng, Santy Ky, Richner Beat, Pommier Jean-David, Sorn San, Chevalier Véronique, Buchy Philippe, De Lamballerie Xavier, Cappelle Julien, Horwood Paul, Dussart Philippe. 2017. Journal of General Virology, 98 : 2287-2296.https://doi.org/10.1099/jgv.0.000892 <https://doi.org/10.1099/jgv.0.000892> Researchers Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs Duong, Veasna Choeung, Rithy Gorman, Christopher Laurent, Denis Crabol, Yoann Mey, Channa Peng, Borin Di Francesco, Juliette Hul, Vibol Sothy, Heng Santy, Ky Richner, Beat Pommier, Jean-David Sorn, San Chevalier, Véronique Buchy, Philippe De Lamballerie, Xavier Cappelle, Julien Horwood, Paul Dussart, Philippe eng 2017 Journal of General Virology S50 - Santé humaine L73 - Maladies des animaux U30 - Méthodes de recherche Cambodge http://aims.fao.org/aos/agrovoc/c_4073 Japanese encephalitis remains the most important cause of viral encephalitis in humans in several southeast Asian countries, including Cambodia, causing at least 65 000 cases of encephalitis per year. This vector-borne viral zoonosis – caused by Japanese encephalitis virus (JEV) – is considered to be a rural disease and is transmitted by mosquitoes, with birds and pigs being the natural reservoirs, while humans are accidental hosts. In this study we report the first two JEV isolations in Cambodia from human encephalitis cases from two studies on the aetiology of central nervous system disease, conducted at the two major paediatric hospitals in the country. We also report JEV isolation from Culextritaeniorhynchus mosquitoes and from pig samples collected in two farms, located in peri-urban and rural areas. Out of 11 reverse-transcription polymerase chain reaction-positive original samples, we generated full-genome sequences from 5 JEV isolates. Five additional partial sequences of the JEV NS3 gene from viruses detected in five pigs and one complete coding sequence of the envelope gene of a strain identified in a pig were generated. Phylogenetic analyses revealed that JEV detected in Cambodia belonged to genotype I and clustered in two clades: genotype I-a, mainly comprising strains from Thailand, and genotype I-b, comprising strains from Vietnam that dispersed northwards to China. Finally, in this study, we provide proof that the sequenced JEV strains circulate between pigs, Culex tritaeniorhynchus and humans in the Phnom Penh vicinity. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/585281/7/2287_vir000892.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1099/jgv.0.000892 10.1099/jgv.0.000892 info:eu-repo/semantics/altIdentifier/doi/10.1099/jgv.0.000892 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1099/jgv.0.000892 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/KY927819 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/KY927814 info:eu-repo/grantAgreement/EC//DCI-ASIE-2013-315-047//ComAcross project/ info:eu-repo/grantAgreement/DG Development and Cooperation - EuropeAid//DCI-ASIE-2013-315-047//ComAcross project/ |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
S50 - Santé humaine L73 - Maladies des animaux U30 - Méthodes de recherche http://aims.fao.org/aos/agrovoc/c_4073 S50 - Santé humaine L73 - Maladies des animaux U30 - Méthodes de recherche http://aims.fao.org/aos/agrovoc/c_4073 |
spellingShingle |
S50 - Santé humaine L73 - Maladies des animaux U30 - Méthodes de recherche http://aims.fao.org/aos/agrovoc/c_4073 S50 - Santé humaine L73 - Maladies des animaux U30 - Méthodes de recherche http://aims.fao.org/aos/agrovoc/c_4073 Duong, Veasna Choeung, Rithy Gorman, Christopher Laurent, Denis Crabol, Yoann Mey, Channa Peng, Borin Di Francesco, Juliette Hul, Vibol Sothy, Heng Santy, Ky Richner, Beat Pommier, Jean-David Sorn, San Chevalier, Véronique Buchy, Philippe De Lamballerie, Xavier Cappelle, Julien Horwood, Paul Dussart, Philippe Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs |
description |
Japanese encephalitis remains the most important cause of viral encephalitis in humans in several southeast Asian countries, including Cambodia, causing at least 65 000 cases of encephalitis per year. This vector-borne viral zoonosis – caused by Japanese encephalitis virus (JEV) – is considered to be a rural disease and is transmitted by mosquitoes, with birds and pigs being the natural reservoirs, while humans are accidental hosts. In this study we report the first two JEV isolations in Cambodia from human encephalitis cases from two studies on the aetiology of central nervous system disease, conducted at the two major paediatric hospitals in the country. We also report JEV isolation from Culextritaeniorhynchus mosquitoes and from pig samples collected in two farms, located in peri-urban and rural areas. Out of 11 reverse-transcription polymerase chain reaction-positive original samples, we generated full-genome sequences from 5 JEV isolates. Five additional partial sequences of the JEV NS3 gene from viruses detected in five pigs and one complete coding sequence of the envelope gene of a strain identified in a pig were generated. Phylogenetic analyses revealed that JEV detected in Cambodia belonged to genotype I and clustered in two clades: genotype I-a, mainly comprising strains from Thailand, and genotype I-b, comprising strains from Vietnam that dispersed northwards to China. Finally, in this study, we provide proof that the sequenced JEV strains circulate between pigs, Culex tritaeniorhynchus and humans in the Phnom Penh vicinity. |
format |
article |
topic_facet |
S50 - Santé humaine L73 - Maladies des animaux U30 - Méthodes de recherche http://aims.fao.org/aos/agrovoc/c_4073 |
author |
Duong, Veasna Choeung, Rithy Gorman, Christopher Laurent, Denis Crabol, Yoann Mey, Channa Peng, Borin Di Francesco, Juliette Hul, Vibol Sothy, Heng Santy, Ky Richner, Beat Pommier, Jean-David Sorn, San Chevalier, Véronique Buchy, Philippe De Lamballerie, Xavier Cappelle, Julien Horwood, Paul Dussart, Philippe |
author_facet |
Duong, Veasna Choeung, Rithy Gorman, Christopher Laurent, Denis Crabol, Yoann Mey, Channa Peng, Borin Di Francesco, Juliette Hul, Vibol Sothy, Heng Santy, Ky Richner, Beat Pommier, Jean-David Sorn, San Chevalier, Véronique Buchy, Philippe De Lamballerie, Xavier Cappelle, Julien Horwood, Paul Dussart, Philippe |
author_sort |
Duong, Veasna |
title |
Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs |
title_short |
Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs |
title_full |
Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs |
title_fullStr |
Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs |
title_full_unstemmed |
Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs |
title_sort |
isolation and full-genome sequences of japanese encephalitis virus genotype i strains from cambodian human patients, mosquitoes and pigs |
url |
http://agritrop.cirad.fr/585281/ http://agritrop.cirad.fr/585281/7/2287_vir000892.pdf |
work_keys_str_mv |
AT duongveasna isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT choeungrithy isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT gormanchristopher isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT laurentdenis isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT crabolyoann isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT meychanna isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT pengborin isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT difrancescojuliette isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT hulvibol isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT sothyheng isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT santyky isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT richnerbeat isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT pommierjeandavid isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT sornsan isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT chevalierveronique isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT buchyphilippe isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT delamballeriexavier isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT cappellejulien isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT horwoodpaul isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs AT dussartphilippe isolationandfullgenomesequencesofjapaneseencephalitisvirusgenotypeistrainsfromcambodianhumanpatientsmosquitoesandpigs |
_version_ |
1758025459689324544 |