Nitrogen fixation in a chemoautotrophic lucinid symbiosis
The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO2 and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont–host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The C. orbicularis symbiont, here tentatively named 'Candidatus Thiodiazotropha endolucinida', may thus not only sustain the bivalve's carbon demands. C. orbicularis may also benefit from a steady supply of fixed nitrogen from its symbiont—a scenario that is unprecedented in comparable chemoautotrophic symbioses.
Main Authors: | , , , , , , , , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | M40 - Écologie aquatique, Fixation de l'azote, Codakia orbicularis, symbiose, Bivalvia, activité enzymatique, symbiote, diazotrophie, http://aims.fao.org/aos/agrovoc/c_5196, http://aims.fao.org/aos/agrovoc/c_48452, http://aims.fao.org/aos/agrovoc/c_7563, http://aims.fao.org/aos/agrovoc/c_942, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_7562, http://aims.fao.org/aos/agrovoc/c_27500, |
Online Access: | http://agritrop.cirad.fr/584642/ http://agritrop.cirad.fr/584642/1/Nitrogen%20Fixation%20Bivalve_Nat%20Microbiology%202017.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-584642 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5846422024-01-29T05:39:32Z http://agritrop.cirad.fr/584642/ http://agritrop.cirad.fr/584642/ Nitrogen fixation in a chemoautotrophic lucinid symbiosis. König Sten, Gros Olivier, Heiden Stefan E., Hinzke Tjorven, Thürmer Thomas, Poehlein Anja, Meyer Susann, Vatin Magalie, Mbéguié-A-Mbéguié Didier, Tocny Jennifer, Ponnudurai Ruby, Daniel Rolf, Becher Dörte, Schweder Thomas, Markert Stephanie. 2017. Nature Microbiology, 2 (1):e16193, 10 p.https://doi.org/10.1038/nmicrobiol.2016.193 <https://doi.org/10.1038/nmicrobiol.2016.193> Nitrogen fixation in a chemoautotrophic lucinid symbiosis König, Sten Gros, Olivier Heiden, Stefan E. Hinzke, Tjorven Thürmer, Thomas Poehlein, Anja Meyer, Susann Vatin, Magalie Mbéguié-A-Mbéguié, Didier Tocny, Jennifer Ponnudurai, Ruby Daniel, Rolf Becher, Dörte Schweder, Thomas Markert, Stephanie eng 2017 Nature Microbiology M40 - Écologie aquatique Fixation de l'azote Codakia orbicularis symbiose Bivalvia activité enzymatique symbiote diazotrophie http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_48452 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_942 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_27500 The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO2 and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont–host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The C. orbicularis symbiont, here tentatively named 'Candidatus Thiodiazotropha endolucinida', may thus not only sustain the bivalve's carbon demands. C. orbicularis may also benefit from a steady supply of fixed nitrogen from its symbiont—a scenario that is unprecedented in comparable chemoautotrophic symbioses. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/584642/1/Nitrogen%20Fixation%20Bivalve_Nat%20Microbiology%202017.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1038/nmicrobiol.2016.193 10.1038/nmicrobiol.2016.193 info:eu-repo/semantics/altIdentifier/doi/10.1038/nmicrobiol.2016.193 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/nmicrobiol.2016.193 info:eu-repo/grantAgreement/EC/FP7/264774//(EU) Molecular Ecology and Evolution of Bacterial Symbionts/SYMBIOMICS |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
M40 - Écologie aquatique Fixation de l'azote Codakia orbicularis symbiose Bivalvia activité enzymatique symbiote diazotrophie http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_48452 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_942 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_27500 M40 - Écologie aquatique Fixation de l'azote Codakia orbicularis symbiose Bivalvia activité enzymatique symbiote diazotrophie http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_48452 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_942 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_27500 |
spellingShingle |
M40 - Écologie aquatique Fixation de l'azote Codakia orbicularis symbiose Bivalvia activité enzymatique symbiote diazotrophie http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_48452 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_942 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_27500 M40 - Écologie aquatique Fixation de l'azote Codakia orbicularis symbiose Bivalvia activité enzymatique symbiote diazotrophie http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_48452 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_942 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_27500 König, Sten Gros, Olivier Heiden, Stefan E. Hinzke, Tjorven Thürmer, Thomas Poehlein, Anja Meyer, Susann Vatin, Magalie Mbéguié-A-Mbéguié, Didier Tocny, Jennifer Ponnudurai, Ruby Daniel, Rolf Becher, Dörte Schweder, Thomas Markert, Stephanie Nitrogen fixation in a chemoautotrophic lucinid symbiosis |
description |
The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO2 and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont–host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The C. orbicularis symbiont, here tentatively named 'Candidatus Thiodiazotropha endolucinida', may thus not only sustain the bivalve's carbon demands. C. orbicularis may also benefit from a steady supply of fixed nitrogen from its symbiont—a scenario that is unprecedented in comparable chemoautotrophic symbioses. |
format |
article |
topic_facet |
M40 - Écologie aquatique Fixation de l'azote Codakia orbicularis symbiose Bivalvia activité enzymatique symbiote diazotrophie http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_48452 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_942 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_27500 |
author |
König, Sten Gros, Olivier Heiden, Stefan E. Hinzke, Tjorven Thürmer, Thomas Poehlein, Anja Meyer, Susann Vatin, Magalie Mbéguié-A-Mbéguié, Didier Tocny, Jennifer Ponnudurai, Ruby Daniel, Rolf Becher, Dörte Schweder, Thomas Markert, Stephanie |
author_facet |
König, Sten Gros, Olivier Heiden, Stefan E. Hinzke, Tjorven Thürmer, Thomas Poehlein, Anja Meyer, Susann Vatin, Magalie Mbéguié-A-Mbéguié, Didier Tocny, Jennifer Ponnudurai, Ruby Daniel, Rolf Becher, Dörte Schweder, Thomas Markert, Stephanie |
author_sort |
König, Sten |
title |
Nitrogen fixation in a chemoautotrophic lucinid symbiosis |
title_short |
Nitrogen fixation in a chemoautotrophic lucinid symbiosis |
title_full |
Nitrogen fixation in a chemoautotrophic lucinid symbiosis |
title_fullStr |
Nitrogen fixation in a chemoautotrophic lucinid symbiosis |
title_full_unstemmed |
Nitrogen fixation in a chemoautotrophic lucinid symbiosis |
title_sort |
nitrogen fixation in a chemoautotrophic lucinid symbiosis |
url |
http://agritrop.cirad.fr/584642/ http://agritrop.cirad.fr/584642/1/Nitrogen%20Fixation%20Bivalve_Nat%20Microbiology%202017.pdf |
work_keys_str_mv |
AT konigsten nitrogenfixationinachemoautotrophiclucinidsymbiosis AT grosolivier nitrogenfixationinachemoautotrophiclucinidsymbiosis AT heidenstefane nitrogenfixationinachemoautotrophiclucinidsymbiosis AT hinzketjorven nitrogenfixationinachemoautotrophiclucinidsymbiosis AT thurmerthomas nitrogenfixationinachemoautotrophiclucinidsymbiosis AT poehleinanja nitrogenfixationinachemoautotrophiclucinidsymbiosis AT meyersusann nitrogenfixationinachemoautotrophiclucinidsymbiosis AT vatinmagalie nitrogenfixationinachemoautotrophiclucinidsymbiosis AT mbeguieambeguiedidier nitrogenfixationinachemoautotrophiclucinidsymbiosis AT tocnyjennifer nitrogenfixationinachemoautotrophiclucinidsymbiosis AT ponnudurairuby nitrogenfixationinachemoautotrophiclucinidsymbiosis AT danielrolf nitrogenfixationinachemoautotrophiclucinidsymbiosis AT becherdorte nitrogenfixationinachemoautotrophiclucinidsymbiosis AT schwederthomas nitrogenfixationinachemoautotrophiclucinidsymbiosis AT markertstephanie nitrogenfixationinachemoautotrophiclucinidsymbiosis |
_version_ |
1792499301918703616 |