Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy
This study aimed to test Raman (400–1800 cm− 1) and Infra-red (1900–500 cm− 1) spectroscopies followed by statistical analysis (principal component analysis) to detect molecular changes induced by antibiotics (ampicillin, cefotaxime – cell wall synthesis inhibitors, tetracycline – protein synthesis inhibitor, ciprofloxacin – DNA synthesis inhibitor) against Escherichia coli TOP10. In case of ampicillin and cefotaxime, a decrease in protein bands in both Raman (1240, 1660 cm− 1), and IR spectra (1230, 1530, 1630 cm− 1), and an increase in carbohydrate bands (1150, 1020 cm− 1) in IR spectra were observed. Tetracycline addition caused an increase in nucleic acid bands (775, 1478, 1578 cm− 1), a sharp decrease in phenylalanine (995 cm− 1) in Raman spectra and the amide I and amide II bands (1630, 1530 cm− 1) in IR spectra, an increase in DNA in both Raman (1083 cm− 1) and IR spectra (1080 cm− 1). Regarding ciprofloxacin, an increase in nucleic acids (775, 1478, 1578 cm− 1) in Raman spectra and in protein bands (1230, 1520, 1630 cm− 1), in DNA (1080 cm− 1) in IR spectra were detected. Clear discrimination of antibiotic-treated samples compared to the control was recorded, showing that Raman and IR spectroscopies, coupled to principal component analysis for data, could be used to detect molecular modifications in bacteria exposed to different classes of antibiotics. These findings contribute to the understanding of the mechanisms of action of antibiotics in bacteria.
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Subjects: | U30 - Méthodes de recherche, L73 - Maladies des animaux, 000 - Autres thèmes, antibiotique, Escherichia coli, céfotaxime, spectroscopie, adn, méthode statistique, Résistance aux antibiotiques, http://aims.fao.org/aos/agrovoc/c_492, http://aims.fao.org/aos/agrovoc/c_33700, http://aims.fao.org/aos/agrovoc/c_36175, http://aims.fao.org/aos/agrovoc/c_14498, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_7377, http://aims.fao.org/aos/agrovoc/c_8848, |
Online Access: | http://agritrop.cirad.fr/584260/ http://agritrop.cirad.fr/584260/1/Xuan%202017-Spectrochimica.pdf |
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dig-cirad-fr-5842602024-01-29T05:39:08Z http://agritrop.cirad.fr/584260/ http://agritrop.cirad.fr/584260/ Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy. Nguyen Ngoc Thanh Xuan, Sarter Samira, Nguyen N. Hai, Daniel Philippe. 2017. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 183 : 395-401.https://doi.org/10.1016/j.saa.2017.04.077 <https://doi.org/10.1016/j.saa.2017.04.077> Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy Nguyen, Ngoc Thanh Xuan Sarter, Samira Nguyen, N. Hai Daniel, Philippe eng 2017 Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy U30 - Méthodes de recherche L73 - Maladies des animaux 000 - Autres thèmes antibiotique Escherichia coli céfotaxime spectroscopie adn méthode statistique Résistance aux antibiotiques http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_36175 http://aims.fao.org/aos/agrovoc/c_14498 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8848 This study aimed to test Raman (400–1800 cm− 1) and Infra-red (1900–500 cm− 1) spectroscopies followed by statistical analysis (principal component analysis) to detect molecular changes induced by antibiotics (ampicillin, cefotaxime – cell wall synthesis inhibitors, tetracycline – protein synthesis inhibitor, ciprofloxacin – DNA synthesis inhibitor) against Escherichia coli TOP10. In case of ampicillin and cefotaxime, a decrease in protein bands in both Raman (1240, 1660 cm− 1), and IR spectra (1230, 1530, 1630 cm− 1), and an increase in carbohydrate bands (1150, 1020 cm− 1) in IR spectra were observed. Tetracycline addition caused an increase in nucleic acid bands (775, 1478, 1578 cm− 1), a sharp decrease in phenylalanine (995 cm− 1) in Raman spectra and the amide I and amide II bands (1630, 1530 cm− 1) in IR spectra, an increase in DNA in both Raman (1083 cm− 1) and IR spectra (1080 cm− 1). Regarding ciprofloxacin, an increase in nucleic acids (775, 1478, 1578 cm− 1) in Raman spectra and in protein bands (1230, 1520, 1630 cm− 1), in DNA (1080 cm− 1) in IR spectra were detected. Clear discrimination of antibiotic-treated samples compared to the control was recorded, showing that Raman and IR spectroscopies, coupled to principal component analysis for data, could be used to detect molecular modifications in bacteria exposed to different classes of antibiotics. These findings contribute to the understanding of the mechanisms of action of antibiotics in bacteria. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/acceptedVersion http://agritrop.cirad.fr/584260/1/Xuan%202017-Spectrochimica.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.saa.2017.04.077 10.1016/j.saa.2017.04.077 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.saa.2017.04.077 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.saa.2017.04.077 |
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U30 - Méthodes de recherche L73 - Maladies des animaux 000 - Autres thèmes antibiotique Escherichia coli céfotaxime spectroscopie adn méthode statistique Résistance aux antibiotiques http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_36175 http://aims.fao.org/aos/agrovoc/c_14498 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8848 U30 - Méthodes de recherche L73 - Maladies des animaux 000 - Autres thèmes antibiotique Escherichia coli céfotaxime spectroscopie adn méthode statistique Résistance aux antibiotiques http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_36175 http://aims.fao.org/aos/agrovoc/c_14498 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8848 |
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U30 - Méthodes de recherche L73 - Maladies des animaux 000 - Autres thèmes antibiotique Escherichia coli céfotaxime spectroscopie adn méthode statistique Résistance aux antibiotiques http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_36175 http://aims.fao.org/aos/agrovoc/c_14498 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8848 U30 - Méthodes de recherche L73 - Maladies des animaux 000 - Autres thèmes antibiotique Escherichia coli céfotaxime spectroscopie adn méthode statistique Résistance aux antibiotiques http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_36175 http://aims.fao.org/aos/agrovoc/c_14498 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8848 Nguyen, Ngoc Thanh Xuan Sarter, Samira Nguyen, N. Hai Daniel, Philippe Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy |
description |
This study aimed to test Raman (400–1800 cm− 1) and Infra-red (1900–500 cm− 1) spectroscopies followed by statistical analysis (principal component analysis) to detect molecular changes induced by antibiotics (ampicillin, cefotaxime – cell wall synthesis inhibitors, tetracycline – protein synthesis inhibitor, ciprofloxacin – DNA synthesis inhibitor) against Escherichia coli TOP10. In case of ampicillin and cefotaxime, a decrease in protein bands in both Raman (1240, 1660 cm− 1), and IR spectra (1230, 1530, 1630 cm− 1), and an increase in carbohydrate bands (1150, 1020 cm− 1) in IR spectra were observed. Tetracycline addition caused an increase in nucleic acid bands (775, 1478, 1578 cm− 1), a sharp decrease in phenylalanine (995 cm− 1) in Raman spectra and the amide I and amide II bands (1630, 1530 cm− 1) in IR spectra, an increase in DNA in both Raman (1083 cm− 1) and IR spectra (1080 cm− 1). Regarding ciprofloxacin, an increase in nucleic acids (775, 1478, 1578 cm− 1) in Raman spectra and in protein bands (1230, 1520, 1630 cm− 1), in DNA (1080 cm− 1) in IR spectra were detected. Clear discrimination of antibiotic-treated samples compared to the control was recorded, showing that Raman and IR spectroscopies, coupled to principal component analysis for data, could be used to detect molecular modifications in bacteria exposed to different classes of antibiotics. These findings contribute to the understanding of the mechanisms of action of antibiotics in bacteria. |
format |
article |
topic_facet |
U30 - Méthodes de recherche L73 - Maladies des animaux 000 - Autres thèmes antibiotique Escherichia coli céfotaxime spectroscopie adn méthode statistique Résistance aux antibiotiques http://aims.fao.org/aos/agrovoc/c_492 http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_36175 http://aims.fao.org/aos/agrovoc/c_14498 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8848 |
author |
Nguyen, Ngoc Thanh Xuan Sarter, Samira Nguyen, N. Hai Daniel, Philippe |
author_facet |
Nguyen, Ngoc Thanh Xuan Sarter, Samira Nguyen, N. Hai Daniel, Philippe |
author_sort |
Nguyen, Ngoc Thanh Xuan |
title |
Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy |
title_short |
Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy |
title_full |
Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy |
title_fullStr |
Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy |
title_full_unstemmed |
Detection of molecular changes induced by antibiotics in Escherichia coli using vibrational spectroscopy |
title_sort |
detection of molecular changes induced by antibiotics in escherichia coli using vibrational spectroscopy |
url |
http://agritrop.cirad.fr/584260/ http://agritrop.cirad.fr/584260/1/Xuan%202017-Spectrochimica.pdf |
work_keys_str_mv |
AT nguyenngocthanhxuan detectionofmolecularchangesinducedbyantibioticsinescherichiacoliusingvibrationalspectroscopy AT sartersamira detectionofmolecularchangesinducedbyantibioticsinescherichiacoliusingvibrationalspectroscopy AT nguyennhai detectionofmolecularchangesinducedbyantibioticsinescherichiacoliusingvibrationalspectroscopy AT danielphilippe detectionofmolecularchangesinducedbyantibioticsinescherichiacoliusingvibrationalspectroscopy |
_version_ |
1792499273182478336 |