Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France)
In addition to being one of the most acute problems impeding chemical control of fungal diseases, the evolution of fungicide resistance is an emblematic case of local adaptation to spatially heterogeneous and temporally variable selection pressures. Here we dissected the adaptation of Botrytis cinerea (the causal agent of grey mould) populations on grapes to several fungicides. We carried out a 2-year survey (four collection dates) on three treated/untreated pairs of plots from vineyards in Champagne (France) and monitored the frequency of four resistant phenotypes that are unambiguously associated with four distinct genotypes. For two loci under selection by currently used fungicides (MDR1 and MDR2), the frequencies of resistant mutations at vintage were greater in treated plots compared to untreated plots, showing that the effect of selection is detectable even at the plot scale. This effect was not detectable for two other loci under selection by previously used fungicides (BenR1 and ImiR1). We also found that treatment with currently used fungicides reduced B. cinerea effective population size, leading to a significant decrease in genic diversity and allelic richness in treated vs. untreated plots. We further highlight that even under ample drift and migration, fungal populations can present an efficient response to selection. Finally, for the four studied loci, the costs of fungicide resistance were estimated by modelling the decrease in the frequency of resistant mutations in the absence of treatment. We discuss the importance of these estimates for defining strategies for limiting or counteracting the local adaptation of pests to fungicides.
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Language: | eng |
Subjects: | H20 - Maladies des plantes, Vitis vinifera, Botrytis cinerea, résistance aux pesticides, modélisation des cultures, fongicide, adaptation, écologie microbienne, dynamique des populations, mécanisme de défense, mutation, http://aims.fao.org/aos/agrovoc/c_8283, http://aims.fao.org/aos/agrovoc/c_26413, http://aims.fao.org/aos/agrovoc/c_25427, http://aims.fao.org/aos/agrovoc/c_9000024, http://aims.fao.org/aos/agrovoc/c_3146, http://aims.fao.org/aos/agrovoc/c_117, http://aims.fao.org/aos/agrovoc/c_24111, http://aims.fao.org/aos/agrovoc/c_6111, http://aims.fao.org/aos/agrovoc/c_35269, http://aims.fao.org/aos/agrovoc/c_5014, http://aims.fao.org/aos/agrovoc/c_1495, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/584179/ http://agritrop.cirad.fr/584179/1/Walker_et_al-2017-Molecular_Ecology.pdf |
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dig-cirad-fr-5841792024-01-29T00:15:05Z http://agritrop.cirad.fr/584179/ http://agritrop.cirad.fr/584179/ Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France). Walker Anne Sophie, Ravigné Virginie, Rieux Adrien, Ali S., Carpentier F., Fournier Elisabeth. 2017. Molecular Ecology, 26 (7), n.spéc. Microbial local adaption : 1919-1935.https://doi.org/10.1111/mec.14072 <https://doi.org/10.1111/mec.14072> Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France) Walker, Anne Sophie Ravigné, Virginie Rieux, Adrien Ali, S. Carpentier, F. Fournier, Elisabeth eng 2017 Molecular Ecology H20 - Maladies des plantes Vitis vinifera Botrytis cinerea résistance aux pesticides modélisation des cultures fongicide adaptation écologie microbienne dynamique des populations mécanisme de défense mutation http://aims.fao.org/aos/agrovoc/c_8283 http://aims.fao.org/aos/agrovoc/c_26413 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_9000024 http://aims.fao.org/aos/agrovoc/c_3146 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_5014 Champagne-Ardennes France http://aims.fao.org/aos/agrovoc/c_1495 http://aims.fao.org/aos/agrovoc/c_3081 In addition to being one of the most acute problems impeding chemical control of fungal diseases, the evolution of fungicide resistance is an emblematic case of local adaptation to spatially heterogeneous and temporally variable selection pressures. Here we dissected the adaptation of Botrytis cinerea (the causal agent of grey mould) populations on grapes to several fungicides. We carried out a 2-year survey (four collection dates) on three treated/untreated pairs of plots from vineyards in Champagne (France) and monitored the frequency of four resistant phenotypes that are unambiguously associated with four distinct genotypes. For two loci under selection by currently used fungicides (MDR1 and MDR2), the frequencies of resistant mutations at vintage were greater in treated plots compared to untreated plots, showing that the effect of selection is detectable even at the plot scale. This effect was not detectable for two other loci under selection by previously used fungicides (BenR1 and ImiR1). We also found that treatment with currently used fungicides reduced B. cinerea effective population size, leading to a significant decrease in genic diversity and allelic richness in treated vs. untreated plots. We further highlight that even under ample drift and migration, fungal populations can present an efficient response to selection. Finally, for the four studied loci, the costs of fungicide resistance were estimated by modelling the decrease in the frequency of resistant mutations in the absence of treatment. We discuss the importance of these estimates for defining strategies for limiting or counteracting the local adaptation of pests to fungicides. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/584179/1/Walker_et_al-2017-Molecular_Ecology.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/mec.14072 10.1111/mec.14072 info:eu-repo/semantics/altIdentifier/doi/10.1111/mec.14072 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/mec.14072 info:eu-repo/semantics/dataset/purl/https://doi.org/10.5061/dryad.g1hm0 |
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H20 - Maladies des plantes Vitis vinifera Botrytis cinerea résistance aux pesticides modélisation des cultures fongicide adaptation écologie microbienne dynamique des populations mécanisme de défense mutation http://aims.fao.org/aos/agrovoc/c_8283 http://aims.fao.org/aos/agrovoc/c_26413 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_9000024 http://aims.fao.org/aos/agrovoc/c_3146 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_1495 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Vitis vinifera Botrytis cinerea résistance aux pesticides modélisation des cultures fongicide adaptation écologie microbienne dynamique des populations mécanisme de défense mutation http://aims.fao.org/aos/agrovoc/c_8283 http://aims.fao.org/aos/agrovoc/c_26413 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_9000024 http://aims.fao.org/aos/agrovoc/c_3146 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_1495 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
H20 - Maladies des plantes Vitis vinifera Botrytis cinerea résistance aux pesticides modélisation des cultures fongicide adaptation écologie microbienne dynamique des populations mécanisme de défense mutation http://aims.fao.org/aos/agrovoc/c_8283 http://aims.fao.org/aos/agrovoc/c_26413 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_9000024 http://aims.fao.org/aos/agrovoc/c_3146 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_1495 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Vitis vinifera Botrytis cinerea résistance aux pesticides modélisation des cultures fongicide adaptation écologie microbienne dynamique des populations mécanisme de défense mutation http://aims.fao.org/aos/agrovoc/c_8283 http://aims.fao.org/aos/agrovoc/c_26413 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_9000024 http://aims.fao.org/aos/agrovoc/c_3146 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_1495 http://aims.fao.org/aos/agrovoc/c_3081 Walker, Anne Sophie Ravigné, Virginie Rieux, Adrien Ali, S. Carpentier, F. Fournier, Elisabeth Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France) |
description |
In addition to being one of the most acute problems impeding chemical control of fungal diseases, the evolution of fungicide resistance is an emblematic case of local adaptation to spatially heterogeneous and temporally variable selection pressures. Here we dissected the adaptation of Botrytis cinerea (the causal agent of grey mould) populations on grapes to several fungicides. We carried out a 2-year survey (four collection dates) on three treated/untreated pairs of plots from vineyards in Champagne (France) and monitored the frequency of four resistant phenotypes that are unambiguously associated with four distinct genotypes. For two loci under selection by currently used fungicides (MDR1 and MDR2), the frequencies of resistant mutations at vintage were greater in treated plots compared to untreated plots, showing that the effect of selection is detectable even at the plot scale. This effect was not detectable for two other loci under selection by previously used fungicides (BenR1 and ImiR1). We also found that treatment with currently used fungicides reduced B. cinerea effective population size, leading to a significant decrease in genic diversity and allelic richness in treated vs. untreated plots. We further highlight that even under ample drift and migration, fungal populations can present an efficient response to selection. Finally, for the four studied loci, the costs of fungicide resistance were estimated by modelling the decrease in the frequency of resistant mutations in the absence of treatment. We discuss the importance of these estimates for defining strategies for limiting or counteracting the local adaptation of pests to fungicides. |
format |
article |
topic_facet |
H20 - Maladies des plantes Vitis vinifera Botrytis cinerea résistance aux pesticides modélisation des cultures fongicide adaptation écologie microbienne dynamique des populations mécanisme de défense mutation http://aims.fao.org/aos/agrovoc/c_8283 http://aims.fao.org/aos/agrovoc/c_26413 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_9000024 http://aims.fao.org/aos/agrovoc/c_3146 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_1495 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Walker, Anne Sophie Ravigné, Virginie Rieux, Adrien Ali, S. Carpentier, F. Fournier, Elisabeth |
author_facet |
Walker, Anne Sophie Ravigné, Virginie Rieux, Adrien Ali, S. Carpentier, F. Fournier, Elisabeth |
author_sort |
Walker, Anne Sophie |
title |
Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France) |
title_short |
Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France) |
title_full |
Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France) |
title_fullStr |
Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France) |
title_full_unstemmed |
Fungal adaptation to contemporary fungicide applications: the case of Botrytis cinerea populations from Champagne vineyards (France) |
title_sort |
fungal adaptation to contemporary fungicide applications: the case of botrytis cinerea populations from champagne vineyards (france) |
url |
http://agritrop.cirad.fr/584179/ http://agritrop.cirad.fr/584179/1/Walker_et_al-2017-Molecular_Ecology.pdf |
work_keys_str_mv |
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