Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding
Cultivated Citrus result from genome admixtures of four ancestral taxa (C. reticula, C. maxima, C. medica and C. micrantha). Phylogenomics is therefore a key to decipher the origin of modern varieties and to understand the molecular basis of phenotypic variability. Next Generation Sequencing technologies have considerably modified our capacity to explore genomic variation and to understand the complex citrus genomic. However, low cost discrete scan of the genome with highly reliable markers may be sufficient to answer numerous research questions. 454 and Sanger sequencing data of gene fragments dispersed on the nine chromosomes were used to select SNPs totally discriminant of each of the four basic taxa (DSNPs). One hundred fifty selected DSNPs were successfully converted into KASPar markers and provided highly reliable genotyping in diploid and polyploid citrus. They were applied to analyse the origin of secondary citrus species and modern varieties. DSNPs are also very useful (i) to routinely identify zygotic seedlings of citrus rootstocks deriving from direct hybridization between ancestral taxa (ie: Sour orange = C. reticulata x C. maxima, Rough lemon, Volkamer lemon, Rangpur lime: C. reticula x C. medica; Alemow: C. micrantha x C. medica),.(ii) to analyse meiotic mechanisms (2n gametes, tetraploid meiosis) of secondary citrus species and (iii) to trace candidate gene phylogeny in citrus germplasm or recombining progenies. (Texte intégral)
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dig-cirad-fr-5835572020-12-15T17:11:40Z http://agritrop.cirad.fr/583557/ http://agritrop.cirad.fr/583557/ Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding. Ollitrault Patrick, Curk Franck, Ollitrault Frédérique, Garcia-Lor Andrés, Navarro Luis, Morillon Raphaël. 2016. In : Abstract book Sustainable citriculture: the role of applied knowledge. Mattos Dirceu (ed.), Fermino Carlos Eduardo (ed.), Moreira Novelli Valdenice (ed.), Alves de Azevedo Fernando (ed.), Della Coletta Filho Helvécio (ed.), Vicente Contador Zaccheo Paulo (ed.). IAC, IAPAR. Londrina : IAPAR, Résumé, 126-127. ISBN 978-85-88184-55-8 International Citrus Congress, Foz do Iguaçu, Brésil, 18 Septembre 2016/23 Septembre 2016. Researchers Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding Ollitrault, Patrick Curk, Franck Ollitrault, Frédérique Garcia-Lor, Andrés Navarro, Luis Morillon, Raphaël eng 2016 IAPAR Abstract book Sustainable citriculture: the role of applied knowledge F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie Cultivated Citrus result from genome admixtures of four ancestral taxa (C. reticula, C. maxima, C. medica and C. micrantha). Phylogenomics is therefore a key to decipher the origin of modern varieties and to understand the molecular basis of phenotypic variability. Next Generation Sequencing technologies have considerably modified our capacity to explore genomic variation and to understand the complex citrus genomic. However, low cost discrete scan of the genome with highly reliable markers may be sufficient to answer numerous research questions. 454 and Sanger sequencing data of gene fragments dispersed on the nine chromosomes were used to select SNPs totally discriminant of each of the four basic taxa (DSNPs). One hundred fifty selected DSNPs were successfully converted into KASPar markers and provided highly reliable genotyping in diploid and polyploid citrus. They were applied to analyse the origin of secondary citrus species and modern varieties. DSNPs are also very useful (i) to routinely identify zygotic seedlings of citrus rootstocks deriving from direct hybridization between ancestral taxa (ie: Sour orange = C. reticulata x C. maxima, Rough lemon, Volkamer lemon, Rangpur lime: C. reticula x C. medica; Alemow: C. micrantha x C. medica),.(ii) to analyse meiotic mechanisms (2n gametes, tetraploid meiosis) of secondary citrus species and (iii) to trace candidate gene phylogeny in citrus germplasm or recombining progenies. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/583557/1/583557.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html |
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F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie |
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F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie Ollitrault, Patrick Curk, Franck Ollitrault, Frédérique Garcia-Lor, Andrés Navarro, Luis Morillon, Raphaël Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding |
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Cultivated Citrus result from genome admixtures of four ancestral taxa (C. reticula, C. maxima, C. medica and C. micrantha). Phylogenomics is therefore a key to decipher the origin of modern varieties and to understand the molecular basis of phenotypic variability. Next Generation Sequencing technologies have considerably modified our capacity to explore genomic variation and to understand the complex citrus genomic. However, low cost discrete scan of the genome with highly reliable markers may be sufficient to answer numerous research questions. 454 and Sanger sequencing data of gene fragments dispersed on the nine chromosomes were used to select SNPs totally discriminant of each of the four basic taxa (DSNPs). One hundred fifty selected DSNPs were successfully converted into KASPar markers and provided highly reliable genotyping in diploid and polyploid citrus. They were applied to analyse the origin of secondary citrus species and modern varieties. DSNPs are also very useful (i) to routinely identify zygotic seedlings of citrus rootstocks deriving from direct hybridization between ancestral taxa (ie: Sour orange = C. reticulata x C. maxima, Rough lemon, Volkamer lemon, Rangpur lime: C. reticula x C. medica; Alemow: C. micrantha x C. medica),.(ii) to analyse meiotic mechanisms (2n gametes, tetraploid meiosis) of secondary citrus species and (iii) to trace candidate gene phylogeny in citrus germplasm or recombining progenies. (Texte intégral) |
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conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie |
author |
Ollitrault, Patrick Curk, Franck Ollitrault, Frédérique Garcia-Lor, Andrés Navarro, Luis Morillon, Raphaël |
author_facet |
Ollitrault, Patrick Curk, Franck Ollitrault, Frédérique Garcia-Lor, Andrés Navarro, Luis Morillon, Raphaël |
author_sort |
Ollitrault, Patrick |
title |
Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding |
title_short |
Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding |
title_full |
Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding |
title_fullStr |
Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding |
title_full_unstemmed |
Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding |
title_sort |
usefulness of phylogentic diagnostic snp markers of citrus ancestral taxa for genetics and breeding |
publisher |
IAPAR |
url |
http://agritrop.cirad.fr/583557/ http://agritrop.cirad.fr/583557/1/583557.pdf |
work_keys_str_mv |
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