Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding

Cultivated Citrus result from genome admixtures of four ancestral taxa (C. reticula, C. maxima, C. medica and C. micrantha). Phylogenomics is therefore a key to decipher the origin of modern varieties and to understand the molecular basis of phenotypic variability. Next Generation Sequencing technologies have considerably modified our capacity to explore genomic variation and to understand the complex citrus genomic. However, low cost discrete scan of the genome with highly reliable markers may be sufficient to answer numerous research questions. 454 and Sanger sequencing data of gene fragments dispersed on the nine chromosomes were used to select SNPs totally discriminant of each of the four basic taxa (DSNPs). One hundred fifty selected DSNPs were successfully converted into KASPar markers and provided highly reliable genotyping in diploid and polyploid citrus. They were applied to analyse the origin of secondary citrus species and modern varieties. DSNPs are also very useful (i) to routinely identify zygotic seedlings of citrus rootstocks deriving from direct hybridization between ancestral taxa (ie: Sour orange = C. reticulata x C. maxima, Rough lemon, Volkamer lemon, Rangpur lime: C. reticula x C. medica; Alemow: C. micrantha x C. medica),.(ii) to analyse meiotic mechanisms (2n gametes, tetraploid meiosis) of secondary citrus species and (iii) to trace candidate gene phylogeny in citrus germplasm or recombining progenies. (Texte intégral)

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Main Authors: Ollitrault, Patrick, Curk, Franck, Ollitrault, Frédérique, Garcia-Lor, Andrés, Navarro, Luis, Morillon, Raphaël
Format: conference_item biblioteca
Language:eng
Published: IAPAR
Subjects:F30 - Génétique et amélioration des plantes, F70 - Taxonomie végétale et phytogéographie,
Online Access:http://agritrop.cirad.fr/583557/
http://agritrop.cirad.fr/583557/1/583557.pdf
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spelling dig-cirad-fr-5835572020-12-15T17:11:40Z http://agritrop.cirad.fr/583557/ http://agritrop.cirad.fr/583557/ Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding. Ollitrault Patrick, Curk Franck, Ollitrault Frédérique, Garcia-Lor Andrés, Navarro Luis, Morillon Raphaël. 2016. In : Abstract book Sustainable citriculture: the role of applied knowledge. Mattos Dirceu (ed.), Fermino Carlos Eduardo (ed.), Moreira Novelli Valdenice (ed.), Alves de Azevedo Fernando (ed.), Della Coletta Filho Helvécio (ed.), Vicente Contador Zaccheo Paulo (ed.). IAC, IAPAR. Londrina : IAPAR, Résumé, 126-127. ISBN 978-85-88184-55-8 International Citrus Congress, Foz do Iguaçu, Brésil, 18 Septembre 2016/23 Septembre 2016. Researchers Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding Ollitrault, Patrick Curk, Franck Ollitrault, Frédérique Garcia-Lor, Andrés Navarro, Luis Morillon, Raphaël eng 2016 IAPAR Abstract book Sustainable citriculture: the role of applied knowledge F30 - Génétique et amélioration des plantes F70 - Taxonomie végétale et phytogéographie Cultivated Citrus result from genome admixtures of four ancestral taxa (C. reticula, C. maxima, C. medica and C. micrantha). Phylogenomics is therefore a key to decipher the origin of modern varieties and to understand the molecular basis of phenotypic variability. Next Generation Sequencing technologies have considerably modified our capacity to explore genomic variation and to understand the complex citrus genomic. However, low cost discrete scan of the genome with highly reliable markers may be sufficient to answer numerous research questions. 454 and Sanger sequencing data of gene fragments dispersed on the nine chromosomes were used to select SNPs totally discriminant of each of the four basic taxa (DSNPs). One hundred fifty selected DSNPs were successfully converted into KASPar markers and provided highly reliable genotyping in diploid and polyploid citrus. They were applied to analyse the origin of secondary citrus species and modern varieties. DSNPs are also very useful (i) to routinely identify zygotic seedlings of citrus rootstocks deriving from direct hybridization between ancestral taxa (ie: Sour orange = C. reticulata x C. maxima, Rough lemon, Volkamer lemon, Rangpur lime: C. reticula x C. medica; Alemow: C. micrantha x C. medica),.(ii) to analyse meiotic mechanisms (2n gametes, tetraploid meiosis) of secondary citrus species and (iii) to trace candidate gene phylogeny in citrus germplasm or recombining progenies. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/583557/1/583557.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html
institution CIRAD FR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cirad-fr
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CIRAD Francia
language eng
topic F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie
F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie
spellingShingle F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie
F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie
Ollitrault, Patrick
Curk, Franck
Ollitrault, Frédérique
Garcia-Lor, Andrés
Navarro, Luis
Morillon, Raphaël
Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding
description Cultivated Citrus result from genome admixtures of four ancestral taxa (C. reticula, C. maxima, C. medica and C. micrantha). Phylogenomics is therefore a key to decipher the origin of modern varieties and to understand the molecular basis of phenotypic variability. Next Generation Sequencing technologies have considerably modified our capacity to explore genomic variation and to understand the complex citrus genomic. However, low cost discrete scan of the genome with highly reliable markers may be sufficient to answer numerous research questions. 454 and Sanger sequencing data of gene fragments dispersed on the nine chromosomes were used to select SNPs totally discriminant of each of the four basic taxa (DSNPs). One hundred fifty selected DSNPs were successfully converted into KASPar markers and provided highly reliable genotyping in diploid and polyploid citrus. They were applied to analyse the origin of secondary citrus species and modern varieties. DSNPs are also very useful (i) to routinely identify zygotic seedlings of citrus rootstocks deriving from direct hybridization between ancestral taxa (ie: Sour orange = C. reticulata x C. maxima, Rough lemon, Volkamer lemon, Rangpur lime: C. reticula x C. medica; Alemow: C. micrantha x C. medica),.(ii) to analyse meiotic mechanisms (2n gametes, tetraploid meiosis) of secondary citrus species and (iii) to trace candidate gene phylogeny in citrus germplasm or recombining progenies. (Texte intégral)
format conference_item
topic_facet F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie
author Ollitrault, Patrick
Curk, Franck
Ollitrault, Frédérique
Garcia-Lor, Andrés
Navarro, Luis
Morillon, Raphaël
author_facet Ollitrault, Patrick
Curk, Franck
Ollitrault, Frédérique
Garcia-Lor, Andrés
Navarro, Luis
Morillon, Raphaël
author_sort Ollitrault, Patrick
title Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding
title_short Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding
title_full Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding
title_fullStr Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding
title_full_unstemmed Usefulness of phylogentic diagnostic SNP markers of citrus ancestral taxa for genetics and breeding
title_sort usefulness of phylogentic diagnostic snp markers of citrus ancestral taxa for genetics and breeding
publisher IAPAR
url http://agritrop.cirad.fr/583557/
http://agritrop.cirad.fr/583557/1/583557.pdf
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