TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469]
Dear biologist, have you ever dreamed of using the whole power of those numerous NGS tools that your bioinformatician colleagues uses through this awful list of command lines ? Dear bioinformatician, have you ever wished for a really quick way of designing a new NGS pipeline without having to retype again dozens of code lines to readapt your scripts or starting from scratch ? So, be happy ! TOGGLE is for you ! With TOGGLE (TOolbox for Generic nGs anaLysEs), you can create your own pipeline through an easy and user-friendly approach. Indeed, TOGGLE integrate a large set of NGS softwares and utilities to easily design pipelines able to handle hundreds of samples. The pipelines can start from Fastq (plain or compressed), SAM, BAM or VCF (plain or compressed) files, with parallel (by sample) and global analyses (by multi samples). Moreover, TOGGLE offers an easy way to configure your pipeline with a single configuration file: – Organizing the different steps of workflow, – Setting the parameters for the different softwares, – Managing storage space through compressing/deleting intermediate data, – Determining the way the jobs are managed (serial or parallel jobs through scheduler SGE, SLURM and LSF). TOGGLE can work on your laptop, on a single machine server as well as on a HPC system, as a local instance or in a Docker machine. The only limit will be your available space on the storage system, not the amount of samples to be treated or the number of steps. TOGGLE was used on different organisms, from a single sample to more than one hundred at a time, in RNAseq, DNAreseq/SNP discovery and GBS analyses. List of bioinformatics tools included: – Cleaning and Quality checking : FastQC, CutAdapt, fastxTrimmer – Assembly : TransAbyss, Trinity, TGI-CL – Mapping : BWA (aln/sampe and mem), TopHat – SAM/BAM management : picardTools, SAMtools, GATK – SNP calling/cleaning/annotation : SAMtools, GATK, VarScan, snpEff – ReadCount : HTseq-count – Structural Variations : BreakDancer, Pindel Web site: https://github.com/SouthGreenPlatform/TOGGLE (Texte integral)
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dig-cirad-fr-5834632022-03-14T17:01:03Z http://agritrop.cirad.fr/583463/ http://agritrop.cirad.fr/583463/ TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469]. Tranchant-Dubreuil Christine, Ravel Sébastien, Monat Cécile, Helou Laura, Diallo Abdoulaye, Sarah Gautier, Orjuela-Bouniol Julie, Sabot François. 2017. In : Proceedings Plant and Animal Genome XXV Conference. San Diego : PAG, 1 p. Plant and Animal Genome Conference. 25, San Diego, États-Unis, 14 Janvier 2017/18 Janvier 2017.https://pag.confex.com/pag/xxv/meetingapp.cgi/Paper/24651 <https://pag.confex.com/pag/xxv/meetingapp.cgi/Paper/24651> Researchers TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469] Tranchant-Dubreuil, Christine Ravel, Sébastien Monat, Cécile Helou, Laura Diallo, Abdoulaye Sarah, Gautier Orjuela-Bouniol, Julie Sabot, François eng 2017 PAG Proceedings Plant and Animal Genome XXV Conference U10 - Informatique, mathématiques et statistiques U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L10 - Génétique et amélioration des animaux Dear biologist, have you ever dreamed of using the whole power of those numerous NGS tools that your bioinformatician colleagues uses through this awful list of command lines ? Dear bioinformatician, have you ever wished for a really quick way of designing a new NGS pipeline without having to retype again dozens of code lines to readapt your scripts or starting from scratch ? So, be happy ! TOGGLE is for you ! With TOGGLE (TOolbox for Generic nGs anaLysEs), you can create your own pipeline through an easy and user-friendly approach. Indeed, TOGGLE integrate a large set of NGS softwares and utilities to easily design pipelines able to handle hundreds of samples. The pipelines can start from Fastq (plain or compressed), SAM, BAM or VCF (plain or compressed) files, with parallel (by sample) and global analyses (by multi samples). Moreover, TOGGLE offers an easy way to configure your pipeline with a single configuration file: – Organizing the different steps of workflow, – Setting the parameters for the different softwares, – Managing storage space through compressing/deleting intermediate data, – Determining the way the jobs are managed (serial or parallel jobs through scheduler SGE, SLURM and LSF). TOGGLE can work on your laptop, on a single machine server as well as on a HPC system, as a local instance or in a Docker machine. The only limit will be your available space on the storage system, not the amount of samples to be treated or the number of steps. TOGGLE was used on different organisms, from a single sample to more than one hundred at a time, in RNAseq, DNAreseq/SNP discovery and GBS analyses. List of bioinformatics tools included: – Cleaning and Quality checking : FastQC, CutAdapt, fastxTrimmer – Assembly : TransAbyss, Trinity, TGI-CL – Mapping : BWA (aln/sampe and mem), TopHat – SAM/BAM management : picardTools, SAMtools, GATK – SNP calling/cleaning/annotation : SAMtools, GATK, VarScan, snpEff – ReadCount : HTseq-count – Structural Variations : BreakDancer, Pindel Web site: https://github.com/SouthGreenPlatform/TOGGLE (Texte integral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/583463/1/ID583463.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://pag.confex.com/pag/xxv/meetingapp.cgi/Paper/24651 info:eu-repo/semantics/altIdentifier/purl/https://pag.confex.com/pag/xxv/meetingapp.cgi/Paper/24651 |
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U10 - Informatique, mathématiques et statistiques U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L10 - Génétique et amélioration des animaux U10 - Informatique, mathématiques et statistiques U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L10 - Génétique et amélioration des animaux |
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U10 - Informatique, mathématiques et statistiques U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L10 - Génétique et amélioration des animaux U10 - Informatique, mathématiques et statistiques U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L10 - Génétique et amélioration des animaux Tranchant-Dubreuil, Christine Ravel, Sébastien Monat, Cécile Helou, Laura Diallo, Abdoulaye Sarah, Gautier Orjuela-Bouniol, Julie Sabot, François TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469] |
description |
Dear biologist, have you ever dreamed of using the whole power of those numerous NGS tools that your bioinformatician colleagues uses through this awful list of command lines ? Dear bioinformatician, have you ever wished for a really quick way of designing a new NGS pipeline without having to retype again dozens of code lines to readapt your scripts or starting from scratch ? So, be happy ! TOGGLE is for you ! With TOGGLE (TOolbox for Generic nGs anaLysEs), you can create your own pipeline through an easy and user-friendly approach. Indeed, TOGGLE integrate a large set of NGS softwares and utilities to easily design pipelines able to handle hundreds of samples. The pipelines can start from Fastq (plain or compressed), SAM, BAM or VCF (plain or compressed) files, with parallel (by sample) and global analyses (by multi samples). Moreover, TOGGLE offers an easy way to configure your pipeline with a single configuration file: – Organizing the different steps of workflow, – Setting the parameters for the different softwares, – Managing storage space through compressing/deleting intermediate data, – Determining the way the jobs are managed (serial or parallel jobs through scheduler SGE, SLURM and LSF). TOGGLE can work on your laptop, on a single machine server as well as on a HPC system, as a local instance or in a Docker machine. The only limit will be your available space on the storage system, not the amount of samples to be treated or the number of steps. TOGGLE was used on different organisms, from a single sample to more than one hundred at a time, in RNAseq, DNAreseq/SNP discovery and GBS analyses. List of bioinformatics tools included: – Cleaning and Quality checking : FastQC, CutAdapt, fastxTrimmer – Assembly : TransAbyss, Trinity, TGI-CL – Mapping : BWA (aln/sampe and mem), TopHat – SAM/BAM management : picardTools, SAMtools, GATK – SNP calling/cleaning/annotation : SAMtools, GATK, VarScan, snpEff – ReadCount : HTseq-count – Structural Variations : BreakDancer, Pindel Web site: https://github.com/SouthGreenPlatform/TOGGLE (Texte integral) |
format |
conference_item |
topic_facet |
U10 - Informatique, mathématiques et statistiques U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes L10 - Génétique et amélioration des animaux |
author |
Tranchant-Dubreuil, Christine Ravel, Sébastien Monat, Cécile Helou, Laura Diallo, Abdoulaye Sarah, Gautier Orjuela-Bouniol, Julie Sabot, François |
author_facet |
Tranchant-Dubreuil, Christine Ravel, Sébastien Monat, Cécile Helou, Laura Diallo, Abdoulaye Sarah, Gautier Orjuela-Bouniol, Julie Sabot, François |
author_sort |
Tranchant-Dubreuil, Christine |
title |
TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469] |
title_short |
TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469] |
title_full |
TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469] |
title_fullStr |
TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469] |
title_full_unstemmed |
TOGGLE-3: A Framework to Build Quickly Pipelines and to Perform Large-Scale NGS Analysis. [P0469] |
title_sort |
toggle-3: a framework to build quickly pipelines and to perform large-scale ngs analysis. [p0469] |
publisher |
PAG |
url |
http://agritrop.cirad.fr/583463/ http://agritrop.cirad.fr/583463/1/ID583463.pdf |
work_keys_str_mv |
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_version_ |
1758025266876121088 |