Concentration and purification of lycopene from watermelon juice by integrated microfiltration-based processes
The concentration of thermosensitive bioactive compounds remains an important challenge. A gentle integrated process including enzymatic maceration, crossflow microfiltration, diafiltration and centrifugation was applied to watermelon juice to obtain a natural extract enriched with lycopene. Microfiltration was performed using ceramic membranes with different pore diameters (0.2 to 1.4 μm), transmembrane pressures (50 to 200 kPa) and temperatures (50 °C and 60 °C). Using the best operating conditions during microfiltration, the permeate flux was close to 110 L·h− 1·m− 2, and the lycopene concentration increased 11-fold in the retentate. Diafiltration allowed for the purification of lycopene up to 25-fold. Centrifugation enhanced the lycopene concentration up to 4-fold. cis-Isomerization remained low and similar to that of raw juice. This integrated process obtained a natural extract of lycopene that was 41 times more concentrated and 34 times purer than the initial juice, yielding an extract with up to 2% all-trans-lycopene on a dry basis.
Main Authors: | Chaparro, Luis, Dhuique-Mayer, Claudie, Castillo, Soraya, Vaillant, Fabrice, Servent, Adrien, Dornier, Manuel |
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Format: | article biblioteca |
Language: | eng |
Subjects: | Q02 - Traitement et conservation des produits alimentaires, microfiltration, purification, lycopène, concentration, http://aims.fao.org/aos/agrovoc/c_27575, http://aims.fao.org/aos/agrovoc/c_6377, http://aims.fao.org/aos/agrovoc/c_28451, http://aims.fao.org/aos/agrovoc/c_1801, |
Online Access: | http://agritrop.cirad.fr/581985/ http://agritrop.cirad.fr/581985/1/ACL%20Concentration%20and%20purification%20of%20lycopene.pdf |
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