Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal.
The objective of this study was to characterize the diversity of arbuscular mycorrhizal fungal (AMF) communities colonizing the roots of Vigna unguiculata (L.) plants cultivated in two different sites in Senegal. Roots of cowpea plants and soil samples were collected from two fields (Ngothie and Diokoul) in the rural community of Dya (Senegal). Microscopic observations of the stained roots indicated a high colonization rate in roots from Ngothie site as compared to those from Diokoul site. The partial small subunit of ribosomal DNA genes was amplified from the genomic DNA extracted from these roots by polymerase chain reaction (PCR) with the universal primer NS31 and a fungal-specific primer AML2. Nucleotide sequence analysis revealed that 22 sequences from Ngothie site and only four sequences from Diokoul site were close to those of known arbuscular mycorrhizal fungi. Also, 47.6% of the clones from Ngothie site and 89.47% from Diokoul site were not close to known AMF. A total of 15 operational taxonomic units (OUT) were identified. Phylogenetic analyses showed that these clones belonged to the genera Glomus, Sclerocystis, Rhizophagus, Scutellospora, Gigaspora, Racocetra, Acaulospora and Redeckera. The genus Glomus is the most represented with six OTU, representing 40% of all OTU.
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Subjects: | P34 - Biologie du sol, F61 - Physiologie végétale - Nutrition, http://aims.fao.org/aos/agrovoc/c_6970, |
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dig-cirad-fr-5810692022-04-15T14:10:56Z http://agritrop.cirad.fr/581069/ http://agritrop.cirad.fr/581069/ Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. Diop Ibou, Ndoye Fatou, Kane Aboubacry, Krasova-Wade Tatiana, Pontiroli Alessandra, Do Rego Francis, Noba Kandioura, Prin Yves. 2015. African Journal of Microbiology Research, 9 (21) : 1409-1418.https://doi.org/10.5897/AJMR2015.7472 <https://doi.org/10.5897/AJMR2015.7472> Researchers Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. Diop, Ibou Ndoye, Fatou Kane, Aboubacry Krasova-Wade, Tatiana Pontiroli, Alessandra Do Rego, Francis Noba, Kandioura Prin, Yves eng 2015 African Journal of Microbiology Research P34 - Biologie du sol F61 - Physiologie végétale - Nutrition Sénégal http://aims.fao.org/aos/agrovoc/c_6970 The objective of this study was to characterize the diversity of arbuscular mycorrhizal fungal (AMF) communities colonizing the roots of Vigna unguiculata (L.) plants cultivated in two different sites in Senegal. Roots of cowpea plants and soil samples were collected from two fields (Ngothie and Diokoul) in the rural community of Dya (Senegal). Microscopic observations of the stained roots indicated a high colonization rate in roots from Ngothie site as compared to those from Diokoul site. The partial small subunit of ribosomal DNA genes was amplified from the genomic DNA extracted from these roots by polymerase chain reaction (PCR) with the universal primer NS31 and a fungal-specific primer AML2. Nucleotide sequence analysis revealed that 22 sequences from Ngothie site and only four sequences from Diokoul site were close to those of known arbuscular mycorrhizal fungi. Also, 47.6% of the clones from Ngothie site and 89.47% from Diokoul site were not close to known AMF. A total of 15 operational taxonomic units (OUT) were identified. Phylogenetic analyses showed that these clones belonged to the genera Glomus, Sclerocystis, Rhizophagus, Scutellospora, Gigaspora, Racocetra, Acaulospora and Redeckera. The genus Glomus is the most represented with six OTU, representing 40% of all OTU. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/581069/1/Diop%20et%20al.%2C2015.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.5897/AJMR2015.7472 10.5897/AJMR2015.7472 info:eu-repo/semantics/altIdentifier/doi/10.5897/AJMR2015.7472 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.5897/AJMR2015.7472 info:eu-repo/semantics/dataset/purl/http://www.ncbi.nlm.nih.gov/nuccore/KC588975 info:eu-repo/semantics/dataset/purl/http://www.ncbi.nlm.nih.gov/nuccore/KC589000 |
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P34 - Biologie du sol F61 - Physiologie végétale - Nutrition http://aims.fao.org/aos/agrovoc/c_6970 P34 - Biologie du sol F61 - Physiologie végétale - Nutrition http://aims.fao.org/aos/agrovoc/c_6970 |
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P34 - Biologie du sol F61 - Physiologie végétale - Nutrition http://aims.fao.org/aos/agrovoc/c_6970 P34 - Biologie du sol F61 - Physiologie végétale - Nutrition http://aims.fao.org/aos/agrovoc/c_6970 Diop, Ibou Ndoye, Fatou Kane, Aboubacry Krasova-Wade, Tatiana Pontiroli, Alessandra Do Rego, Francis Noba, Kandioura Prin, Yves Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. |
description |
The objective of this study was to characterize the diversity of arbuscular mycorrhizal fungal (AMF) communities colonizing the roots of Vigna unguiculata (L.) plants cultivated in two different sites in Senegal. Roots of cowpea plants and soil samples were collected from two fields (Ngothie and Diokoul) in the rural community of Dya (Senegal). Microscopic observations of the stained roots indicated a high colonization rate in roots from Ngothie site as compared to those from Diokoul site. The partial small subunit of ribosomal DNA genes was amplified from the genomic DNA extracted from these roots by polymerase chain reaction (PCR) with the universal primer NS31 and a fungal-specific primer AML2. Nucleotide sequence analysis revealed that 22 sequences from Ngothie site and only four sequences from Diokoul site were close to those of known arbuscular mycorrhizal fungi. Also, 47.6% of the clones from Ngothie site and 89.47% from Diokoul site were not close to known AMF. A total of 15 operational taxonomic units (OUT) were identified. Phylogenetic analyses showed that these clones belonged to the genera Glomus, Sclerocystis, Rhizophagus, Scutellospora, Gigaspora, Racocetra, Acaulospora and Redeckera. The genus Glomus is the most represented with six OTU, representing 40% of all OTU. |
format |
article |
topic_facet |
P34 - Biologie du sol F61 - Physiologie végétale - Nutrition http://aims.fao.org/aos/agrovoc/c_6970 |
author |
Diop, Ibou Ndoye, Fatou Kane, Aboubacry Krasova-Wade, Tatiana Pontiroli, Alessandra Do Rego, Francis Noba, Kandioura Prin, Yves |
author_facet |
Diop, Ibou Ndoye, Fatou Kane, Aboubacry Krasova-Wade, Tatiana Pontiroli, Alessandra Do Rego, Francis Noba, Kandioura Prin, Yves |
author_sort |
Diop, Ibou |
title |
Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. |
title_short |
Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. |
title_full |
Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. |
title_fullStr |
Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. |
title_full_unstemmed |
Arbuscular mycorrhizal fungi (AMF) communities associated with cowpea in two ecological site conditions in Senegal. |
title_sort |
arbuscular mycorrhizal fungi (amf) communities associated with cowpea in two ecological site conditions in senegal. |
url |
http://agritrop.cirad.fr/581069/ http://agritrop.cirad.fr/581069/1/Diop%20et%20al.%2C2015.pdf |
work_keys_str_mv |
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