Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale
Background: This work aimed at characterizing 12 isolates of the genus Tuber including Tuber melanosporum (11 isolates) and Tuber brumale (one isolate). This was done using internal transcribed spacer (ITS) sequences, confirming their origin. Results: Analysis of their mating type revealed that both MAT1-1 and MAT1-2 exist within these isolates (with 3 and 8 of each, respectively). We observed that each of these cultures was consistently associated with one bacterium that was intimately linked to fungal growth. These bacterial associates failed to grow in the absence of fungus. We extracted DNA from bacterial colonies in the margin of mycelium and sequenced a nearly complete 16S rDNA gene and a partial ITS fragment. We found they all belonged to the genus Rhodopseudomonas, fitting within different phylogenetic clusters. No relationships were evidenced between bacterial and fungal strains or mating types. Rhodopseudomonas being a sister genus to Bradyrhizobium, we tested the nodulation ability of these bacteria on a promiscuously nodulating legume (Acacia mangium), without success. We failed to identify any nifH genes among these isolates, using two different sets of primers. Conclusions: While the mechanisms of interaction between Tuber and Rhodopseudomonas remain to be elucidated, their interdependency for in vitro growth seems a novel feature of this fungus.
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Language: | eng |
Subjects: | P34 - Biologie du sol, Tuber melanosporum, Rhodopseudomonas, Tuber brumale, Acacia mangium, tubercule, Fixation de l'azote, http://aims.fao.org/aos/agrovoc/c_35521, http://aims.fao.org/aos/agrovoc/c_16029, http://aims.fao.org/aos/agrovoc/c_c8714d6b, http://aims.fao.org/aos/agrovoc/c_42, http://aims.fao.org/aos/agrovoc/c_7998, http://aims.fao.org/aos/agrovoc/c_5196, |
Online Access: | http://agritrop.cirad.fr/581068/ http://agritrop.cirad.fr/581068/1/2016_Le%20Roux%20et%20al.pdf |
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dig-cirad-fr-5810682024-01-29T05:35:32Z http://agritrop.cirad.fr/581068/ http://agritrop.cirad.fr/581068/ Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale. Le Roux Christine, Tournier Estelle, Lies Adrien, Sanguin Hervé, Chevalier Gérard, Duponnois Robin, Mousain Daniel, Prin Yves. 2016. SpringerPlus, 5 (1085), 10 p.https://doi.org/10.1186/s40064-016-2756-6 <https://doi.org/10.1186/s40064-016-2756-6> Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale Le Roux, Christine Tournier, Estelle Lies, Adrien Sanguin, Hervé Chevalier, Gérard Duponnois, Robin Mousain, Daniel Prin, Yves eng 2016 SpringerPlus P34 - Biologie du sol Tuber melanosporum Rhodopseudomonas Tuber brumale Acacia mangium tubercule Fixation de l'azote http://aims.fao.org/aos/agrovoc/c_35521 http://aims.fao.org/aos/agrovoc/c_16029 http://aims.fao.org/aos/agrovoc/c_c8714d6b http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_7998 http://aims.fao.org/aos/agrovoc/c_5196 Background: This work aimed at characterizing 12 isolates of the genus Tuber including Tuber melanosporum (11 isolates) and Tuber brumale (one isolate). This was done using internal transcribed spacer (ITS) sequences, confirming their origin. Results: Analysis of their mating type revealed that both MAT1-1 and MAT1-2 exist within these isolates (with 3 and 8 of each, respectively). We observed that each of these cultures was consistently associated with one bacterium that was intimately linked to fungal growth. These bacterial associates failed to grow in the absence of fungus. We extracted DNA from bacterial colonies in the margin of mycelium and sequenced a nearly complete 16S rDNA gene and a partial ITS fragment. We found they all belonged to the genus Rhodopseudomonas, fitting within different phylogenetic clusters. No relationships were evidenced between bacterial and fungal strains or mating types. Rhodopseudomonas being a sister genus to Bradyrhizobium, we tested the nodulation ability of these bacteria on a promiscuously nodulating legume (Acacia mangium), without success. We failed to identify any nifH genes among these isolates, using two different sets of primers. Conclusions: While the mechanisms of interaction between Tuber and Rhodopseudomonas remain to be elucidated, their interdependency for in vitro growth seems a novel feature of this fungus. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/581068/1/2016_Le%20Roux%20et%20al.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/s40064-016-2756-6 10.1186/s40064-016-2756-6 info:eu-repo/semantics/altIdentifier/doi/10.1186/s40064-016-2756-6 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/s40064-016-2756-6 |
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P34 - Biologie du sol Tuber melanosporum Rhodopseudomonas Tuber brumale Acacia mangium tubercule Fixation de l'azote http://aims.fao.org/aos/agrovoc/c_35521 http://aims.fao.org/aos/agrovoc/c_16029 http://aims.fao.org/aos/agrovoc/c_c8714d6b http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_7998 http://aims.fao.org/aos/agrovoc/c_5196 P34 - Biologie du sol Tuber melanosporum Rhodopseudomonas Tuber brumale Acacia mangium tubercule Fixation de l'azote http://aims.fao.org/aos/agrovoc/c_35521 http://aims.fao.org/aos/agrovoc/c_16029 http://aims.fao.org/aos/agrovoc/c_c8714d6b http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_7998 http://aims.fao.org/aos/agrovoc/c_5196 |
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P34 - Biologie du sol Tuber melanosporum Rhodopseudomonas Tuber brumale Acacia mangium tubercule Fixation de l'azote http://aims.fao.org/aos/agrovoc/c_35521 http://aims.fao.org/aos/agrovoc/c_16029 http://aims.fao.org/aos/agrovoc/c_c8714d6b http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_7998 http://aims.fao.org/aos/agrovoc/c_5196 P34 - Biologie du sol Tuber melanosporum Rhodopseudomonas Tuber brumale Acacia mangium tubercule Fixation de l'azote http://aims.fao.org/aos/agrovoc/c_35521 http://aims.fao.org/aos/agrovoc/c_16029 http://aims.fao.org/aos/agrovoc/c_c8714d6b http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_7998 http://aims.fao.org/aos/agrovoc/c_5196 Le Roux, Christine Tournier, Estelle Lies, Adrien Sanguin, Hervé Chevalier, Gérard Duponnois, Robin Mousain, Daniel Prin, Yves Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale |
description |
Background: This work aimed at characterizing 12 isolates of the genus Tuber including Tuber melanosporum (11 isolates) and Tuber brumale (one isolate). This was done using internal transcribed spacer (ITS) sequences, confirming their origin. Results: Analysis of their mating type revealed that both MAT1-1 and MAT1-2 exist within these isolates (with 3 and 8 of each, respectively). We observed that each of these cultures was consistently associated with one bacterium that was intimately linked to fungal growth. These bacterial associates failed to grow in the absence of fungus. We extracted DNA from bacterial colonies in the margin of mycelium and sequenced a nearly complete 16S rDNA gene and a partial ITS fragment. We found they all belonged to the genus Rhodopseudomonas, fitting within different phylogenetic clusters. No relationships were evidenced between bacterial and fungal strains or mating types. Rhodopseudomonas being a sister genus to Bradyrhizobium, we tested the nodulation ability of these bacteria on a promiscuously nodulating legume (Acacia mangium), without success. We failed to identify any nifH genes among these isolates, using two different sets of primers. Conclusions: While the mechanisms of interaction between Tuber and Rhodopseudomonas remain to be elucidated, their interdependency for in vitro growth seems a novel feature of this fungus. |
format |
article |
topic_facet |
P34 - Biologie du sol Tuber melanosporum Rhodopseudomonas Tuber brumale Acacia mangium tubercule Fixation de l'azote http://aims.fao.org/aos/agrovoc/c_35521 http://aims.fao.org/aos/agrovoc/c_16029 http://aims.fao.org/aos/agrovoc/c_c8714d6b http://aims.fao.org/aos/agrovoc/c_42 http://aims.fao.org/aos/agrovoc/c_7998 http://aims.fao.org/aos/agrovoc/c_5196 |
author |
Le Roux, Christine Tournier, Estelle Lies, Adrien Sanguin, Hervé Chevalier, Gérard Duponnois, Robin Mousain, Daniel Prin, Yves |
author_facet |
Le Roux, Christine Tournier, Estelle Lies, Adrien Sanguin, Hervé Chevalier, Gérard Duponnois, Robin Mousain, Daniel Prin, Yves |
author_sort |
Le Roux, Christine |
title |
Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale |
title_short |
Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale |
title_full |
Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale |
title_fullStr |
Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale |
title_full_unstemmed |
Bacteria of the genus Rhodopseudomonas (Bradyrhizobiaceae): Obligate symbionts in mycelial cultures of the black truffles Tuber melanosporum and Tuber brumale |
title_sort |
bacteria of the genus rhodopseudomonas (bradyrhizobiaceae): obligate symbionts in mycelial cultures of the black truffles tuber melanosporum and tuber brumale |
url |
http://agritrop.cirad.fr/581068/ http://agritrop.cirad.fr/581068/1/2016_Le%20Roux%20et%20al.pdf |
work_keys_str_mv |
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