Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation
Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides (Mmm), is a severe respiratory disease of cattle responsible for major economic losses in sub-Saharan Africa. Disease control relies mainly on the use of empirically attenuated vaccines that provide limited protection. Thus, understanding the virulence mechanisms used by Mmm as well as the role of the host immune system in disease development, persistence, and control is a prerequisite for the development of new, rationally designed control strategies. The aim of this study was to assess the use of whole blood transcriptome analysis to study cattle-Mmm interactions, starting by the characterization of the bovine response to Mmm infection during the acute form of the disease. For that purpose, we compared the transcriptome profile of whole blood from six cattle, before challenge by contact with Mmm-infected animals and at the appearance of first clinical signs, using a bovine microarray. Functional analysis revealed that 680 annotated genes were differentially expressed, with an overwhelming majority of down-regulated genes characterizing an immunosuppression. The main bio-functions affected were "organismal survival", "cellular development, morphology and functions" and "cell-to cell signaling and interactions". These affected functions were consistent with the results of previous in vitro immunological studies. However, microarray and qPCR validation results did not highlight pro-inflammatory molecules (such as TNFα, TLR2, IL-12B and IL-6), whereas inflammation is one of the most characteristic traits of acute CBPP. This global gene expression pattern may be considered as the result, in blood, of the local pulmonary response and the systemic events occurring during acute CBPP. Nevertheless, to understand the immune events occurring during disease, detailed analyses on the different immune cell subpopulations, either in vivo, at the local site, or in vitro, will be required. Whole blood transcriptome analysis remains an interesting approach for the identification of bio-signatures correlating to recovery and protection, which should facilitate the evaluation and validation of novel vaccine formulations.
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dig-cirad-fr-5809642022-04-15T14:10:18Z http://agritrop.cirad.fr/580964/ http://agritrop.cirad.fr/580964/ Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation. Rodrigues Valérie, Holzmuller Philippe, Puech Carinne, Wesonga Hezron, Thiaucourt François, Manso-Silvan Lucia. 2016. In : Xth Symposium of the French network for domestic animal immunology = 10es Journées du réseau français Immunologie des Animaux Domestiques (IAD2016). ANSES. Maisons-Alfort : ANSES, Résumé, p. 34. Symposium on the French Network for Domestic Animal Immunology (IAD 2016). 10, Ploufragan, France, 17 Mars 2016/18 Mars 2016. Researchers Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation Rodrigues, Valérie Holzmuller, Philippe Puech, Carinne Wesonga, Hezron Thiaucourt, François Manso-Silvan, Lucia eng 2016 ANSES Xth Symposium of the French network for domestic animal immunology L73 - Maladies des animaux L50 - Physiologie et biochimie animales Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides (Mmm), is a severe respiratory disease of cattle responsible for major economic losses in sub-Saharan Africa. Disease control relies mainly on the use of empirically attenuated vaccines that provide limited protection. Thus, understanding the virulence mechanisms used by Mmm as well as the role of the host immune system in disease development, persistence, and control is a prerequisite for the development of new, rationally designed control strategies. The aim of this study was to assess the use of whole blood transcriptome analysis to study cattle-Mmm interactions, starting by the characterization of the bovine response to Mmm infection during the acute form of the disease. For that purpose, we compared the transcriptome profile of whole blood from six cattle, before challenge by contact with Mmm-infected animals and at the appearance of first clinical signs, using a bovine microarray. Functional analysis revealed that 680 annotated genes were differentially expressed, with an overwhelming majority of down-regulated genes characterizing an immunosuppression. The main bio-functions affected were "organismal survival", "cellular development, morphology and functions" and "cell-to cell signaling and interactions". These affected functions were consistent with the results of previous in vitro immunological studies. However, microarray and qPCR validation results did not highlight pro-inflammatory molecules (such as TNFα, TLR2, IL-12B and IL-6), whereas inflammation is one of the most characteristic traits of acute CBPP. This global gene expression pattern may be considered as the result, in blood, of the local pulmonary response and the systemic events occurring during acute CBPP. Nevertheless, to understand the immune events occurring during disease, detailed analyses on the different immune cell subpopulations, either in vivo, at the local site, or in vitro, will be required. Whole blood transcriptome analysis remains an interesting approach for the identification of bio-signatures correlating to recovery and protection, which should facilitate the evaluation and validation of novel vaccine formulations. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/580964/7/ID580964.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html info:eu-repo/semantics/reference/purl/https://www.anses.fr/fr/content/iad-2016 |
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L73 - Maladies des animaux L50 - Physiologie et biochimie animales L73 - Maladies des animaux L50 - Physiologie et biochimie animales Rodrigues, Valérie Holzmuller, Philippe Puech, Carinne Wesonga, Hezron Thiaucourt, François Manso-Silvan, Lucia Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation |
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Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides (Mmm), is a severe respiratory disease of cattle responsible for major economic losses in sub-Saharan Africa. Disease control relies mainly on the use of empirically attenuated vaccines that provide limited protection. Thus, understanding the virulence mechanisms used by Mmm as well as the role of the host immune system in disease development, persistence, and control is a prerequisite for the development of new, rationally designed control strategies. The aim of this study was to assess the use of whole blood transcriptome analysis to study cattle-Mmm interactions, starting by the characterization of the bovine response to Mmm infection during the acute form of the disease. For that purpose, we compared the transcriptome profile of whole blood from six cattle, before challenge by contact with Mmm-infected animals and at the appearance of first clinical signs, using a bovine microarray. Functional analysis revealed that 680 annotated genes were differentially expressed, with an overwhelming majority of down-regulated genes characterizing an immunosuppression. The main bio-functions affected were "organismal survival", "cellular development, morphology and functions" and "cell-to cell signaling and interactions". These affected functions were consistent with the results of previous in vitro immunological studies. However, microarray and qPCR validation results did not highlight pro-inflammatory molecules (such as TNFα, TLR2, IL-12B and IL-6), whereas inflammation is one of the most characteristic traits of acute CBPP. This global gene expression pattern may be considered as the result, in blood, of the local pulmonary response and the systemic events occurring during acute CBPP. Nevertheless, to understand the immune events occurring during disease, detailed analyses on the different immune cell subpopulations, either in vivo, at the local site, or in vitro, will be required. Whole blood transcriptome analysis remains an interesting approach for the identification of bio-signatures correlating to recovery and protection, which should facilitate the evaluation and validation of novel vaccine formulations. |
format |
conference_item |
topic_facet |
L73 - Maladies des animaux L50 - Physiologie et biochimie animales |
author |
Rodrigues, Valérie Holzmuller, Philippe Puech, Carinne Wesonga, Hezron Thiaucourt, François Manso-Silvan, Lucia |
author_facet |
Rodrigues, Valérie Holzmuller, Philippe Puech, Carinne Wesonga, Hezron Thiaucourt, François Manso-Silvan, Lucia |
author_sort |
Rodrigues, Valérie |
title |
Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation |
title_short |
Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation |
title_full |
Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation |
title_fullStr |
Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation |
title_full_unstemmed |
Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation |
title_sort |
whole blood transcriptome analysis of mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation |
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ANSES |
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http://agritrop.cirad.fr/580964/ http://agritrop.cirad.fr/580964/7/ID580964.pdf |
work_keys_str_mv |
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