Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia
The taxonomic status of eleven rhizospheric bacterial strains belonging to the genus Burkholderia and isolated from roots of Costularia (Cyperaceae), tropical herbaceous pioneer plants growing on ultramafic soils in New Caledonia, was investigated using a polyphasic taxonomic approach. The genetic analyses (16S rRNA genes, gyrB, recA, nreB and cnr) confirmed that all strains are Burkholderia and cluster into two separated groups. The DNA hybridization results showed low relatedness values to the closest relatives Burkholderia species. The phenotypic analyses confirmed that the two groups of strains could be differentiated from each other and from other known Burkholderia species. This polyphasic study revealed that these two groups of strains represent each a novel species of Burkholderia, for which the names Burkholderia novacaledonica sp. nov. (type strain STM10272T = LMG28615T = CIP110887T) and B. ultramafica sp. nov. (type strain STM10279T = LMG28614T = CIP110886T) are proposed, respectively. These strains of Burkholderia presented specific ecological traits such as the tolerance to the extreme edaphic constraints of ultramafic soils: they grew at pH between 4 and 8 and tolerate the strong unbalanced Ca/Mg ratio (1/19) and the high concentrations of heavy metals i.e. Co, Cr, Mn and Ni. Noteworthy B. ultramafica tolerated nickel until 10 mM and B. novacaledonica up to 5 mM. The presence of the nickel (nreB) and cobalt/nickel (cnr) resistance determinants encoding for protein involved in metal tolerance was found in all strains of both groups. Moreover, most of the strains were able to produce plant growth promoting molecules (ACC, IAA, NH3 and siderophores). Such ecological traits suggest that these new species of Burkholderia might be environmentally adaptable plant-associated bacteria and beneficial to plants.
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P34 - Biologie du sol F70 - Taxonomie végétale et phytogéographie F40 - Écologie végétale P36 - Érosion, conservation et récupération des sols F60 - Physiologie et biochimie végétale Rhizobactérie Burkholderiaceae flore bactérienne flore du sol Cyperaceae mycorhization type de sol chimique métal lourd tolérance adaptation génétique des populations résistance génétique variation génétique taxonomie facteur édaphique biodiversité nickel cobalt récupération des sols http://aims.fao.org/aos/agrovoc/c_25068 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_7172 http://aims.fao.org/aos/agrovoc/c_2072 http://aims.fao.org/aos/agrovoc/c_36163 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_15617 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1702 http://aims.fao.org/aos/agrovoc/c_6474 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 P34 - Biologie du sol F70 - Taxonomie végétale et phytogéographie F40 - Écologie végétale P36 - Érosion, conservation et récupération des sols F60 - Physiologie et biochimie végétale Rhizobactérie Burkholderiaceae flore bactérienne flore du sol Cyperaceae mycorhization type de sol chimique métal lourd tolérance adaptation génétique des populations résistance génétique variation génétique taxonomie facteur édaphique biodiversité nickel cobalt récupération des sols http://aims.fao.org/aos/agrovoc/c_25068 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_7172 http://aims.fao.org/aos/agrovoc/c_2072 http://aims.fao.org/aos/agrovoc/c_36163 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_15617 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1702 http://aims.fao.org/aos/agrovoc/c_6474 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 |
spellingShingle |
P34 - Biologie du sol F70 - Taxonomie végétale et phytogéographie F40 - Écologie végétale P36 - Érosion, conservation et récupération des sols F60 - Physiologie et biochimie végétale Rhizobactérie Burkholderiaceae flore bactérienne flore du sol Cyperaceae mycorhization type de sol chimique métal lourd tolérance adaptation génétique des populations résistance génétique variation génétique taxonomie facteur édaphique biodiversité nickel cobalt récupération des sols http://aims.fao.org/aos/agrovoc/c_25068 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_7172 http://aims.fao.org/aos/agrovoc/c_2072 http://aims.fao.org/aos/agrovoc/c_36163 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_15617 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1702 http://aims.fao.org/aos/agrovoc/c_6474 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 P34 - Biologie du sol F70 - Taxonomie végétale et phytogéographie F40 - Écologie végétale P36 - Érosion, conservation et récupération des sols F60 - Physiologie et biochimie végétale Rhizobactérie Burkholderiaceae flore bactérienne flore du sol Cyperaceae mycorhization type de sol chimique métal lourd tolérance adaptation génétique des populations résistance génétique variation génétique taxonomie facteur édaphique biodiversité nickel cobalt récupération des sols http://aims.fao.org/aos/agrovoc/c_25068 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_7172 http://aims.fao.org/aos/agrovoc/c_2072 http://aims.fao.org/aos/agrovoc/c_36163 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_15617 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1702 http://aims.fao.org/aos/agrovoc/c_6474 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 Guentas, Linda Gensous, Simon Cavaloc, Yvon Ducousso, Marc Amir, Hamid De Georges de Ledenon, Benjamin Moulin, Lionel Jourand, Philippe Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia |
description |
The taxonomic status of eleven rhizospheric bacterial strains belonging to the genus Burkholderia and isolated from roots of Costularia (Cyperaceae), tropical herbaceous pioneer plants growing on ultramafic soils in New Caledonia, was investigated using a polyphasic taxonomic approach. The genetic analyses (16S rRNA genes, gyrB, recA, nreB and cnr) confirmed that all strains are Burkholderia and cluster into two separated groups. The DNA hybridization results showed low relatedness values to the closest relatives Burkholderia species. The phenotypic analyses confirmed that the two groups of strains could be differentiated from each other and from other known Burkholderia species. This polyphasic study revealed that these two groups of strains represent each a novel species of Burkholderia, for which the names Burkholderia novacaledonica sp. nov. (type strain STM10272T = LMG28615T = CIP110887T) and B. ultramafica sp. nov. (type strain STM10279T = LMG28614T = CIP110886T) are proposed, respectively. These strains of Burkholderia presented specific ecological traits such as the tolerance to the extreme edaphic constraints of ultramafic soils: they grew at pH between 4 and 8 and tolerate the strong unbalanced Ca/Mg ratio (1/19) and the high concentrations of heavy metals i.e. Co, Cr, Mn and Ni. Noteworthy B. ultramafica tolerated nickel until 10 mM and B. novacaledonica up to 5 mM. The presence of the nickel (nreB) and cobalt/nickel (cnr) resistance determinants encoding for protein involved in metal tolerance was found in all strains of both groups. Moreover, most of the strains were able to produce plant growth promoting molecules (ACC, IAA, NH3 and siderophores). Such ecological traits suggest that these new species of Burkholderia might be environmentally adaptable plant-associated bacteria and beneficial to plants. |
format |
article |
topic_facet |
P34 - Biologie du sol F70 - Taxonomie végétale et phytogéographie F40 - Écologie végétale P36 - Érosion, conservation et récupération des sols F60 - Physiologie et biochimie végétale Rhizobactérie Burkholderiaceae flore bactérienne flore du sol Cyperaceae mycorhization type de sol chimique métal lourd tolérance adaptation génétique des populations résistance génétique variation génétique taxonomie facteur édaphique biodiversité nickel cobalt récupération des sols http://aims.fao.org/aos/agrovoc/c_25068 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_7172 http://aims.fao.org/aos/agrovoc/c_2072 http://aims.fao.org/aos/agrovoc/c_36163 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_15617 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1702 http://aims.fao.org/aos/agrovoc/c_6474 http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Guentas, Linda Gensous, Simon Cavaloc, Yvon Ducousso, Marc Amir, Hamid De Georges de Ledenon, Benjamin Moulin, Lionel Jourand, Philippe |
author_facet |
Guentas, Linda Gensous, Simon Cavaloc, Yvon Ducousso, Marc Amir, Hamid De Georges de Ledenon, Benjamin Moulin, Lionel Jourand, Philippe |
author_sort |
Guentas, Linda |
title |
Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia |
title_short |
Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia |
title_full |
Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia |
title_fullStr |
Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia |
title_full_unstemmed |
Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia |
title_sort |
burkholderia novacaledonica sp. nov. and b. ultramafica sp. nov. isolated from roots of costularia spp. pioneer plants of ultramafic soils in new caledonia |
url |
http://agritrop.cirad.fr/580371/ http://agritrop.cirad.fr/580371/1/580371.pdf |
work_keys_str_mv |
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dig-cirad-fr-5803712024-01-28T23:27:32Z http://agritrop.cirad.fr/580371/ http://agritrop.cirad.fr/580371/ Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia. Guentas Linda, Gensous Simon, Cavaloc Yvon, Ducousso Marc, Amir Hamid, De Georges de Ledenon Benjamin, Moulin Lionel, Jourand Philippe. 2016. Systematic and Applied Microbiology, 39 (3) : 151-159.https://doi.org/10.1016/j.syapm.2016.03.008 <https://doi.org/10.1016/j.syapm.2016.03.008> Burkholderia novacaledonica sp. nov. and B. ultramafica sp. nov. isolated from roots of Costularia spp. pioneer plants of ultramafic soils in New Caledonia Guentas, Linda Gensous, Simon Cavaloc, Yvon Ducousso, Marc Amir, Hamid De Georges de Ledenon, Benjamin Moulin, Lionel Jourand, Philippe eng 2016 Systematic and Applied Microbiology P34 - Biologie du sol F70 - Taxonomie végétale et phytogéographie F40 - Écologie végétale P36 - Érosion, conservation et récupération des sols F60 - Physiologie et biochimie végétale Rhizobactérie Burkholderiaceae flore bactérienne flore du sol Cyperaceae mycorhization type de sol chimique métal lourd tolérance adaptation génétique des populations résistance génétique variation génétique taxonomie facteur édaphique biodiversité nickel cobalt récupération des sols http://aims.fao.org/aos/agrovoc/c_25068 http://aims.fao.org/aos/agrovoc/c_37846 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_7172 http://aims.fao.org/aos/agrovoc/c_2072 http://aims.fao.org/aos/agrovoc/c_36163 http://aims.fao.org/aos/agrovoc/c_1519 http://aims.fao.org/aos/agrovoc/c_2223 http://aims.fao.org/aos/agrovoc/c_37740 http://aims.fao.org/aos/agrovoc/c_117 http://aims.fao.org/aos/agrovoc/c_34326 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_15617 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_5172 http://aims.fao.org/aos/agrovoc/c_1702 http://aims.fao.org/aos/agrovoc/c_6474 Nouvelle-Calédonie France http://aims.fao.org/aos/agrovoc/c_5155 http://aims.fao.org/aos/agrovoc/c_3081 The taxonomic status of eleven rhizospheric bacterial strains belonging to the genus Burkholderia and isolated from roots of Costularia (Cyperaceae), tropical herbaceous pioneer plants growing on ultramafic soils in New Caledonia, was investigated using a polyphasic taxonomic approach. The genetic analyses (16S rRNA genes, gyrB, recA, nreB and cnr) confirmed that all strains are Burkholderia and cluster into two separated groups. The DNA hybridization results showed low relatedness values to the closest relatives Burkholderia species. The phenotypic analyses confirmed that the two groups of strains could be differentiated from each other and from other known Burkholderia species. This polyphasic study revealed that these two groups of strains represent each a novel species of Burkholderia, for which the names Burkholderia novacaledonica sp. nov. (type strain STM10272T = LMG28615T = CIP110887T) and B. ultramafica sp. nov. (type strain STM10279T = LMG28614T = CIP110886T) are proposed, respectively. These strains of Burkholderia presented specific ecological traits such as the tolerance to the extreme edaphic constraints of ultramafic soils: they grew at pH between 4 and 8 and tolerate the strong unbalanced Ca/Mg ratio (1/19) and the high concentrations of heavy metals i.e. Co, Cr, Mn and Ni. Noteworthy B. ultramafica tolerated nickel until 10 mM and B. novacaledonica up to 5 mM. The presence of the nickel (nreB) and cobalt/nickel (cnr) resistance determinants encoding for protein involved in metal tolerance was found in all strains of both groups. Moreover, most of the strains were able to produce plant growth promoting molecules (ACC, IAA, NH3 and siderophores). Such ecological traits suggest that these new species of Burkholderia might be environmentally adaptable plant-associated bacteria and beneficial to plants. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/580371/1/580371.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.syapm.2016.03.008 10.1016/j.syapm.2016.03.008 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.syapm.2016.03.008 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.syapm.2016.03.008 |