Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil
The use of insecticidal proteins known as Cry or Bt, either as biopesticides used in agriculture or as vector control or originating from commercial genetically modified crops (GM), is increasing rapidly. The fate of these proteins in the environment depends strongly on their adsorption on the organo–mineral complexes of soil. Environmental monitoring requires the quantification of the proteins and this entails their chemical extraction from soil. Three Cry proteins, Cry1Ac, Cry1C and Cry2A, present in commercial biopesticide formulations or synthesized by GM plants or both were studied. The adsorption of trace amounts of Cry proteins on over 40 types of soil with contrasting properties was measured in dilute suspension. After a short incubation the extraction yield was measured with a previously tested alkaline solution that contained surfactant and another protein. Each of the proteins had a strong affinity for soil. No soil property was observed to determine either the affinity for soil or the extraction yield. There was no simple relation between the affinity (assessed from the distribution coefficient, Kd) and the extraction yield, although there was a significant inverse relation (P < 0.05) for two of the proteins, Cry1Ac and Cry2A. The proteins differ in both their affinity for soil and their extraction yields. We conclude that these insecticidal proteins will be largely immobile in soil, but that routine environmental monitoring can give only semi-quantitative values for protein in soil.
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Subjects: | H02 - Pesticides, P02 - Pollution, P33 - Chimie et physique du sol, Bacillus thuringiensis, biopesticide, protéine bactérienne, type de sol, persistance des pesticides, pollution du sol, pollution par l'agriculture, adsorption, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_27467, http://aims.fao.org/aos/agrovoc/c_24141, http://aims.fao.org/aos/agrovoc/c_7204, http://aims.fao.org/aos/agrovoc/c_5735, http://aims.fao.org/aos/agrovoc/c_7183, http://aims.fao.org/aos/agrovoc/c_28744, http://aims.fao.org/aos/agrovoc/c_137, http://aims.fao.org/aos/agrovoc/c_3081, |
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dig-cirad-fr-5800332024-01-28T23:23:42Z http://agritrop.cirad.fr/580033/ http://agritrop.cirad.fr/580033/ Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil. Hung Truong Phuc, Truong Le Van, Binh Ngo Dinh, Frutos Roger, Quiquampoix Hervé, Staunton Siobhán. 2016. European Journal of Soil Science, 67 (1), n.spéc. issue on Including Landmark Papers 5 : 90-98.https://doi.org/10.1111/ejss.12313 <https://doi.org/10.1111/ejss.12313> Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán eng 2016 European Journal of Soil Science H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide protéine bactérienne type de sol persistance des pesticides pollution du sol pollution par l'agriculture adsorption http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_7204 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_137 France http://aims.fao.org/aos/agrovoc/c_3081 The use of insecticidal proteins known as Cry or Bt, either as biopesticides used in agriculture or as vector control or originating from commercial genetically modified crops (GM), is increasing rapidly. The fate of these proteins in the environment depends strongly on their adsorption on the organo–mineral complexes of soil. Environmental monitoring requires the quantification of the proteins and this entails their chemical extraction from soil. Three Cry proteins, Cry1Ac, Cry1C and Cry2A, present in commercial biopesticide formulations or synthesized by GM plants or both were studied. The adsorption of trace amounts of Cry proteins on over 40 types of soil with contrasting properties was measured in dilute suspension. After a short incubation the extraction yield was measured with a previously tested alkaline solution that contained surfactant and another protein. Each of the proteins had a strong affinity for soil. No soil property was observed to determine either the affinity for soil or the extraction yield. There was no simple relation between the affinity (assessed from the distribution coefficient, Kd) and the extraction yield, although there was a significant inverse relation (P < 0.05) for two of the proteins, Cry1Ac and Cry2A. The proteins differ in both their affinity for soil and their extraction yields. We conclude that these insecticidal proteins will be largely immobile in soil, but that routine environmental monitoring can give only semi-quantitative values for protein in soil. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/580033/1/580033.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/ejss.12313 10.1111/ejss.12313 info:eu-repo/semantics/altIdentifier/doi/10.1111/ejss.12313 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/ejss.12313 |
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H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide protéine bactérienne type de sol persistance des pesticides pollution du sol pollution par l'agriculture adsorption http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_7204 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_3081 H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide protéine bactérienne type de sol persistance des pesticides pollution du sol pollution par l'agriculture adsorption http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_7204 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_3081 |
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H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide protéine bactérienne type de sol persistance des pesticides pollution du sol pollution par l'agriculture adsorption http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_7204 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_3081 H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide protéine bactérienne type de sol persistance des pesticides pollution du sol pollution par l'agriculture adsorption http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_7204 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_3081 Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil |
description |
The use of insecticidal proteins known as Cry or Bt, either as biopesticides used in agriculture or as vector control or originating from commercial genetically modified crops (GM), is increasing rapidly. The fate of these proteins in the environment depends strongly on their adsorption on the organo–mineral complexes of soil. Environmental monitoring requires the quantification of the proteins and this entails their chemical extraction from soil. Three Cry proteins, Cry1Ac, Cry1C and Cry2A, present in commercial biopesticide formulations or synthesized by GM plants or both were studied. The adsorption of trace amounts of Cry proteins on over 40 types of soil with contrasting properties was measured in dilute suspension. After a short incubation the extraction yield was measured with a previously tested alkaline solution that contained surfactant and another protein. Each of the proteins had a strong affinity for soil. No soil property was observed to determine either the affinity for soil or the extraction yield. There was no simple relation between the affinity (assessed from the distribution coefficient, Kd) and the extraction yield, although there was a significant inverse relation (P < 0.05) for two of the proteins, Cry1Ac and Cry2A. The proteins differ in both their affinity for soil and their extraction yields. We conclude that these insecticidal proteins will be largely immobile in soil, but that routine environmental monitoring can give only semi-quantitative values for protein in soil. |
format |
article |
topic_facet |
H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide protéine bactérienne type de sol persistance des pesticides pollution du sol pollution par l'agriculture adsorption http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_7204 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán |
author_facet |
Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán |
author_sort |
Hung, Truong Phuc |
title |
Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil |
title_short |
Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil |
title_full |
Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil |
title_fullStr |
Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil |
title_full_unstemmed |
Comparison of the affinity and extraction yield of trace amounts of three Cry proteins from Bacillus thuringiensis in contrasting types of soil |
title_sort |
comparison of the affinity and extraction yield of trace amounts of three cry proteins from bacillus thuringiensis in contrasting types of soil |
url |
http://agritrop.cirad.fr/580033/ http://agritrop.cirad.fr/580033/1/580033.pdf |
work_keys_str_mv |
AT hungtruongphuc comparisonoftheaffinityandextractionyieldoftraceamountsofthreecryproteinsfrombacillusthuringiensisincontrastingtypesofsoil AT truonglevan comparisonoftheaffinityandextractionyieldoftraceamountsofthreecryproteinsfrombacillusthuringiensisincontrastingtypesofsoil AT binhngodinh comparisonoftheaffinityandextractionyieldoftraceamountsofthreecryproteinsfrombacillusthuringiensisincontrastingtypesofsoil AT frutosroger comparisonoftheaffinityandextractionyieldoftraceamountsofthreecryproteinsfrombacillusthuringiensisincontrastingtypesofsoil AT quiquampoixherve comparisonoftheaffinityandextractionyieldoftraceamountsofthreecryproteinsfrombacillusthuringiensisincontrastingtypesofsoil AT stauntonsiobhan comparisonoftheaffinityandextractionyieldoftraceamountsofthreecryproteinsfrombacillusthuringiensisincontrastingtypesofsoil |
_version_ |
1792499025297014784 |