Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway
The gaseous plant hormone ethylene has a wide variety of applications in agriculture and horticulture. Ethephon, an ethylene releaser, is used to stimulate natural rubber production in Hevea brasiliensis latex cells. Ethylene Response Factors (ERF) are the last transcription factors if the ethylene signalling pathway and control a large number of ethylene-responsive genes. Two Hevea ERF, HbERF-IXc4 ans HbERF-IXc5, are orthologs to ERF1 a jey regulator at the crosstalk of ethylene and jasmonate signalling pathways. These genes were suggested to play an important role in regulating latex cell metabolism in response to tapping and ethephon stimulation. We regenerated transgenic lines overexpressing HbERF-IXc4 ans HbERF-IXc5 under the control of two promoters, 35S CaMV and HEV2.1, respectively. The latter was shown to direct latex-specific expression in non-photosynthetic tissues. The somatic embryogenesis process was affected by these modifications. however, successful plant regeneration has been obtained. Further characterization of this plant material is in progress to understand the function HbERF-IXc4 ans HbERF-IXc5 in latex. (Texte intégral)
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Prince of Songkla University
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Subjects: | F62 - Physiologie végétale - Croissance et développement, F30 - Génétique et amélioration des plantes, F02 - Multiplication végétative des plantes, |
Online Access: | http://agritrop.cirad.fr/579157/ http://agritrop.cirad.fr/579157/1/Lestari%202015%20IRRDB%20Conference.pdf |
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dig-cirad-fr-5791572021-09-23T07:04:54Z http://agritrop.cirad.fr/579157/ http://agritrop.cirad.fr/579157/ Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway. Lestari Retno, Rio Maryannick, Martin Florence, Pirrello Julien, Dessailly Florence, Leclercq Julie, Suharsono Sony, Montoro Pascal. 2015. In : APRC Asia Pacific Rubber Conference 2015. Phuket : Prince of Songkla University, Résumé, 1 p. Asia Pacific Rubber Conference. 2, Phuket, Thaïlande, 15 Octobre 2015/16 Octobre 2015. Researchers Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway Lestari, Retno Rio, Maryannick Martin, Florence Pirrello, Julien Dessailly, Florence Leclercq, Julie Suharsono, Sony Montoro, Pascal eng 2015 Prince of Songkla University APRC Asia Pacific Rubber Conference 2015 F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes The gaseous plant hormone ethylene has a wide variety of applications in agriculture and horticulture. Ethephon, an ethylene releaser, is used to stimulate natural rubber production in Hevea brasiliensis latex cells. Ethylene Response Factors (ERF) are the last transcription factors if the ethylene signalling pathway and control a large number of ethylene-responsive genes. Two Hevea ERF, HbERF-IXc4 ans HbERF-IXc5, are orthologs to ERF1 a jey regulator at the crosstalk of ethylene and jasmonate signalling pathways. These genes were suggested to play an important role in regulating latex cell metabolism in response to tapping and ethephon stimulation. We regenerated transgenic lines overexpressing HbERF-IXc4 ans HbERF-IXc5 under the control of two promoters, 35S CaMV and HEV2.1, respectively. The latter was shown to direct latex-specific expression in non-photosynthetic tissues. The somatic embryogenesis process was affected by these modifications. however, successful plant regeneration has been obtained. Further characterization of this plant material is in progress to understand the function HbERF-IXc4 ans HbERF-IXc5 in latex. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/579157/1/Lestari%202015%20IRRDB%20Conference.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html |
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F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes |
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F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes Lestari, Retno Rio, Maryannick Martin, Florence Pirrello, Julien Dessailly, Florence Leclercq, Julie Suharsono, Sony Montoro, Pascal Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway |
description |
The gaseous plant hormone ethylene has a wide variety of applications in agriculture and horticulture. Ethephon, an ethylene releaser, is used to stimulate natural rubber production in Hevea brasiliensis latex cells. Ethylene Response Factors (ERF) are the last transcription factors if the ethylene signalling pathway and control a large number of ethylene-responsive genes. Two Hevea ERF, HbERF-IXc4 ans HbERF-IXc5, are orthologs to ERF1 a jey regulator at the crosstalk of ethylene and jasmonate signalling pathways. These genes were suggested to play an important role in regulating latex cell metabolism in response to tapping and ethephon stimulation. We regenerated transgenic lines overexpressing HbERF-IXc4 ans HbERF-IXc5 under the control of two promoters, 35S CaMV and HEV2.1, respectively. The latter was shown to direct latex-specific expression in non-photosynthetic tissues. The somatic embryogenesis process was affected by these modifications. however, successful plant regeneration has been obtained. Further characterization of this plant material is in progress to understand the function HbERF-IXc4 ans HbERF-IXc5 in latex. (Texte intégral) |
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conference_item |
topic_facet |
F62 - Physiologie végétale - Croissance et développement F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes |
author |
Lestari, Retno Rio, Maryannick Martin, Florence Pirrello, Julien Dessailly, Florence Leclercq, Julie Suharsono, Sony Montoro, Pascal |
author_facet |
Lestari, Retno Rio, Maryannick Martin, Florence Pirrello, Julien Dessailly, Florence Leclercq, Julie Suharsono, Sony Montoro, Pascal |
author_sort |
Lestari, Retno |
title |
Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway |
title_short |
Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway |
title_full |
Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway |
title_fullStr |
Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway |
title_full_unstemmed |
Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway |
title_sort |
production of hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway |
publisher |
Prince of Songkla University |
url |
http://agritrop.cirad.fr/579157/ http://agritrop.cirad.fr/579157/1/Lestari%202015%20IRRDB%20Conference.pdf |
work_keys_str_mv |
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