Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis
Hevea brasiliensisis cloned by grafting on non-selected rootstock material. In-vitro techniques such as microcutting and somatic embryogenesis were used for propagation of self-rooted plant material. However, these non-commercial techniques are still restricted to a few numbers of clones. To date, long-term somatic embryogenesis is the most promising technique for large-scale propagation. At CIRAD, successful plant regeneration and genetic modification have been obtained for clone PB 260. This paper aims at developing and cryopreserving embryogenic callus lines for clone RRIM 600. Culture conditions were modified for suitable callus growth and embryogenic induction of the low multiplication rate of RRIM600 callus compared with the clone PB 260 one. Several friable callus lines with high embryogenic potential were selected and cryopreserved in large quantity for further field test and functional genomics studies. Application of this technology to other commercial clones can be now considered based on this experience on clones PB 260 and RRIM 600.
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Prince of Songkla University
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Subjects: | F02 - Multiplication végétative des plantes, F30 - Génétique et amélioration des plantes, |
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dig-cirad-fr-5791542022-04-15T12:54:23Z http://agritrop.cirad.fr/579154/ http://agritrop.cirad.fr/579154/ Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis. Dessailly Florence, Besrest Pierre, Martin Florence, Lardet Ludovic, Granet Françoise, Rio Maryannick, Montoro Pascal. 2015. . Phuket : Prince of Songkla University, 4 p. Asia Pacific Rubber Conference. 2, Phuket, Thaïlande, 15 Octobre 2015/16 Octobre 2015. Researchers Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis Dessailly, Florence Besrest, Pierre Martin, Florence Lardet, Ludovic Granet, Françoise Rio, Maryannick Montoro, Pascal eng 2015 Prince of Songkla University F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Hevea brasiliensisis cloned by grafting on non-selected rootstock material. In-vitro techniques such as microcutting and somatic embryogenesis were used for propagation of self-rooted plant material. However, these non-commercial techniques are still restricted to a few numbers of clones. To date, long-term somatic embryogenesis is the most promising technique for large-scale propagation. At CIRAD, successful plant regeneration and genetic modification have been obtained for clone PB 260. This paper aims at developing and cryopreserving embryogenic callus lines for clone RRIM 600. Culture conditions were modified for suitable callus growth and embryogenic induction of the low multiplication rate of RRIM600 callus compared with the clone PB 260 one. Several friable callus lines with high embryogenic potential were selected and cryopreserved in large quantity for further field test and functional genomics studies. Application of this technology to other commercial clones can be now considered based on this experience on clones PB 260 and RRIM 600. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/579154/1/Establishment%20and%20cryopreservation%20of%20embryogenic%20callus%20lines%20for%20clone%20RRIM%20600%20in%20Hevea%20brasiliensis.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html |
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F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes Dessailly, Florence Besrest, Pierre Martin, Florence Lardet, Ludovic Granet, Françoise Rio, Maryannick Montoro, Pascal Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis |
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Hevea brasiliensisis cloned by grafting on non-selected rootstock material. In-vitro techniques such as microcutting and somatic embryogenesis were used for propagation of self-rooted plant material. However, these non-commercial techniques are still restricted to a few numbers of clones. To date, long-term somatic embryogenesis is the most promising technique for large-scale propagation. At CIRAD, successful plant regeneration and genetic modification have been obtained for clone PB 260. This paper aims at developing and cryopreserving embryogenic callus lines for clone RRIM 600. Culture conditions were modified for suitable callus growth and embryogenic induction of the low multiplication rate of RRIM600 callus compared with the clone PB 260 one. Several friable callus lines with high embryogenic potential were selected and cryopreserved in large quantity for further field test and functional genomics studies. Application of this technology to other commercial clones can be now considered based on this experience on clones PB 260 and RRIM 600. |
format |
conference_item |
topic_facet |
F02 - Multiplication végétative des plantes F30 - Génétique et amélioration des plantes |
author |
Dessailly, Florence Besrest, Pierre Martin, Florence Lardet, Ludovic Granet, Françoise Rio, Maryannick Montoro, Pascal |
author_facet |
Dessailly, Florence Besrest, Pierre Martin, Florence Lardet, Ludovic Granet, Françoise Rio, Maryannick Montoro, Pascal |
author_sort |
Dessailly, Florence |
title |
Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis |
title_short |
Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis |
title_full |
Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis |
title_fullStr |
Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis |
title_full_unstemmed |
Establishment and cryopreservation of embryogenic callus lines for clone RRIM 600 in Hevea brasiliensis |
title_sort |
establishment and cryopreservation of embryogenic callus lines for clone rrim 600 in hevea brasiliensis |
publisher |
Prince of Songkla University |
url |
http://agritrop.cirad.fr/579154/ http://agritrop.cirad.fr/579154/1/Establishment%20and%20cryopreservation%20of%20embryogenic%20callus%20lines%20for%20clone%20RRIM%20600%20in%20Hevea%20brasiliensis.pdf |
work_keys_str_mv |
AT dessaillyflorence establishmentandcryopreservationofembryogeniccalluslinesforclonerrim600inheveabrasiliensis AT besrestpierre establishmentandcryopreservationofembryogeniccalluslinesforclonerrim600inheveabrasiliensis AT martinflorence establishmentandcryopreservationofembryogeniccalluslinesforclonerrim600inheveabrasiliensis AT lardetludovic establishmentandcryopreservationofembryogeniccalluslinesforclonerrim600inheveabrasiliensis AT granetfrancoise establishmentandcryopreservationofembryogeniccalluslinesforclonerrim600inheveabrasiliensis AT riomaryannick establishmentandcryopreservationofembryogeniccalluslinesforclonerrim600inheveabrasiliensis AT montoropascal establishmentandcryopreservationofembryogeniccalluslinesforclonerrim600inheveabrasiliensis |
_version_ |
1758024818649726976 |