Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus
Background Receptor-like kinases are well-known to play key roles in disease resistance. Among them, the Wall-associated kinases (WAKs) have been shown to be positive regulators of fungal disease resistance in several plant species. WAK genes are often transcriptionally regulated during infection but the pathways involved in this regulation are not known. In rice, the OsWAK gene family is significantly amplified compared to Arabidopsis. The possibility that several WAKs participate in different ways to basal defense has not been addressed. Moreover, the direct requirement of rice OSWAK genes in regulating defense has not been explored. Results Here we show using rice (Oryza sativa) loss-of-function mutants of four selected OsWAK genes, that individual OsWAKs are required for quantitative resistance to the rice blast fungus, Magnaporthe oryzae. While OsWAK14, OsWAK91 and OsWAK92 positively regulate quantitative resistance, OsWAK112d is a negative regulator of blast resistance. In addition, we show that the very early transcriptional regulation of the rice OsWAK genes is triggered by chitin and is partially under the control of the chitin receptor CEBiP. Finally, we show that OsWAK91 is required for H2O2 production and sufficient to enhance defense gene expression during infection. Conclusions We conclude that the rice OsWAK genes studied are part of basal defense response, potentially mediated by chitin from fungal cell walls. This work also shows that some OsWAKs, like OsWAK112d, may act as negative regulators of disease resistance.
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dig-cirad-fr-5791182024-01-28T23:13:10Z http://agritrop.cirad.fr/579118/ http://agritrop.cirad.fr/579118/ Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus. Delteil Amandine, Gobbato Enrico, Cayrol Bastien, Estevan Joan, Michel-Romiti Corinne, Diévart Anne, Kroj Thomas, Morel Jean-Benoit. 2016. BMC Plant Biology, 16 (17), 10 p.https://doi.org/10.1186/s12870-016-0711-x <https://doi.org/10.1186/s12870-016-0711-x> Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus Delteil, Amandine Gobbato, Enrico Cayrol, Bastien Estevan, Joan Michel-Romiti, Corinne Diévart, Anne Kroj, Thomas Morel, Jean-Benoit eng 2016 BMC Plant Biology H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Magnaporthe grisea Oryza sativa résistance aux maladies résistance génétique expression des gènes mécanisme de défense cellulaire kinase chitine mutation mutant analyse quantitative interactions biologiques fonction physiologique immunité génie génétique PCR Transcription génique transfert de gène Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_37090 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_1561 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_32660 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_5837 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_33292 France http://aims.fao.org/aos/agrovoc/c_3081 Background Receptor-like kinases are well-known to play key roles in disease resistance. Among them, the Wall-associated kinases (WAKs) have been shown to be positive regulators of fungal disease resistance in several plant species. WAK genes are often transcriptionally regulated during infection but the pathways involved in this regulation are not known. In rice, the OsWAK gene family is significantly amplified compared to Arabidopsis. The possibility that several WAKs participate in different ways to basal defense has not been addressed. Moreover, the direct requirement of rice OSWAK genes in regulating defense has not been explored. Results Here we show using rice (Oryza sativa) loss-of-function mutants of four selected OsWAK genes, that individual OsWAKs are required for quantitative resistance to the rice blast fungus, Magnaporthe oryzae. While OsWAK14, OsWAK91 and OsWAK92 positively regulate quantitative resistance, OsWAK112d is a negative regulator of blast resistance. In addition, we show that the very early transcriptional regulation of the rice OsWAK genes is triggered by chitin and is partially under the control of the chitin receptor CEBiP. Finally, we show that OsWAK91 is required for H2O2 production and sufficient to enhance defense gene expression during infection. Conclusions We conclude that the rice OsWAK genes studied are part of basal defense response, potentially mediated by chitin from fungal cell walls. This work also shows that some OsWAKs, like OsWAK112d, may act as negative regulators of disease resistance. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/579118/1/AOI%20-%20Delteil-Morel%20-%20BMC%20Plant%20Biology%20-%202016-16-1%20.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/s12870-016-0711-x 10.1186/s12870-016-0711-x info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-016-0711-x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/s12870-016-0711-x |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Magnaporthe grisea Oryza sativa résistance aux maladies résistance génétique expression des gènes mécanisme de défense cellulaire kinase chitine mutation mutant analyse quantitative interactions biologiques fonction physiologique immunité génie génétique PCR Transcription génique transfert de gène Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_37090 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_1561 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_32660 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_5837 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Magnaporthe grisea Oryza sativa résistance aux maladies résistance génétique expression des gènes mécanisme de défense cellulaire kinase chitine mutation mutant analyse quantitative interactions biologiques fonction physiologique immunité génie génétique PCR Transcription génique transfert de gène Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_37090 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_1561 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_32660 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_5837 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3081 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Magnaporthe grisea Oryza sativa résistance aux maladies résistance génétique expression des gènes mécanisme de défense cellulaire kinase chitine mutation mutant analyse quantitative interactions biologiques fonction physiologique immunité génie génétique PCR Transcription génique transfert de gène Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_37090 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_1561 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_32660 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_5837 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Magnaporthe grisea Oryza sativa résistance aux maladies résistance génétique expression des gènes mécanisme de défense cellulaire kinase chitine mutation mutant analyse quantitative interactions biologiques fonction physiologique immunité génie génétique PCR Transcription génique transfert de gène Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_37090 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_1561 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_32660 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_5837 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3081 Delteil, Amandine Gobbato, Enrico Cayrol, Bastien Estevan, Joan Michel-Romiti, Corinne Diévart, Anne Kroj, Thomas Morel, Jean-Benoit Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus |
description |
Background Receptor-like kinases are well-known to play key roles in disease resistance. Among them, the Wall-associated kinases (WAKs) have been shown to be positive regulators of fungal disease resistance in several plant species. WAK genes are often transcriptionally regulated during infection but the pathways involved in this regulation are not known. In rice, the OsWAK gene family is significantly amplified compared to Arabidopsis. The possibility that several WAKs participate in different ways to basal defense has not been addressed. Moreover, the direct requirement of rice OSWAK genes in regulating defense has not been explored. Results Here we show using rice (Oryza sativa) loss-of-function mutants of four selected OsWAK genes, that individual OsWAKs are required for quantitative resistance to the rice blast fungus, Magnaporthe oryzae. While OsWAK14, OsWAK91 and OsWAK92 positively regulate quantitative resistance, OsWAK112d is a negative regulator of blast resistance. In addition, we show that the very early transcriptional regulation of the rice OsWAK genes is triggered by chitin and is partially under the control of the chitin receptor CEBiP. Finally, we show that OsWAK91 is required for H2O2 production and sufficient to enhance defense gene expression during infection. Conclusions We conclude that the rice OsWAK genes studied are part of basal defense response, potentially mediated by chitin from fungal cell walls. This work also shows that some OsWAKs, like OsWAK112d, may act as negative regulators of disease resistance. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Magnaporthe grisea Oryza sativa résistance aux maladies résistance génétique expression des gènes mécanisme de défense cellulaire kinase chitine mutation mutant analyse quantitative interactions biologiques fonction physiologique immunité génie génétique PCR Transcription génique transfert de gène Arabidopsis thaliana http://aims.fao.org/aos/agrovoc/c_37090 http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_1561 http://aims.fao.org/aos/agrovoc/c_5014 http://aims.fao.org/aos/agrovoc/c_5013 http://aims.fao.org/aos/agrovoc/c_32660 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_5837 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_24846 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Delteil, Amandine Gobbato, Enrico Cayrol, Bastien Estevan, Joan Michel-Romiti, Corinne Diévart, Anne Kroj, Thomas Morel, Jean-Benoit |
author_facet |
Delteil, Amandine Gobbato, Enrico Cayrol, Bastien Estevan, Joan Michel-Romiti, Corinne Diévart, Anne Kroj, Thomas Morel, Jean-Benoit |
author_sort |
Delteil, Amandine |
title |
Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus |
title_short |
Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus |
title_full |
Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus |
title_fullStr |
Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus |
title_full_unstemmed |
Several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus |
title_sort |
several wall-associated kinases participate positively and negatively in basal defense against rice blast fungus |
url |
http://agritrop.cirad.fr/579118/ http://agritrop.cirad.fr/579118/1/AOI%20-%20Delteil-Morel%20-%20BMC%20Plant%20Biology%20-%202016-16-1%20.pdf |
work_keys_str_mv |
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_version_ |
1792498975147819008 |