Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection
Cocoa black pod caused by Phytophthora is one of the most serious diseases affecting cocoa production. This project set out was to improve our knowledge of the molecular mechanisms involved in Theobroma cacao resistance. We performed a transcriptional screening using macroarrays containing 89 ESTs from subtractive libraries enriched with genes differentially expressed in response to T. cacao/Phytophthora interactions. A gene expression analysis revealed the induction of genes involved in stress signal transduction, general and specific defence genes and genes involved in hormone signalling pathways. RT-qPCR showed that the level expression of two genes in the resistant plant was higher during the first eight hours after pathogen inoculation. Three genes from a family encoding a protease inhibitor displayed different levels of expression depending on resistant and susceptible T. cacao clones. A comparison of promoter sequences revealed a motif present only in genes that were up-expressed in the resistant plant.
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dig-cirad-fr-5774142024-04-30T09:06:51Z http://agritrop.cirad.fr/577414/ http://agritrop.cirad.fr/577414/ Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection. Legavre Thierry, Ducamp Michel, Sabau Xavier, Argout Xavier, Fouet Olivier, Dedieu Frédéric, Surujdeo-Maharaj Surendra, Garcia Dominique, Paulin Didier, Lanaud Claire. 2015. Physiological and Molecular Plant Pathology, 92 : 1-13.https://doi.org/10.1016/j.pmpp.2015.08.005 <https://doi.org/10.1016/j.pmpp.2015.08.005> Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection Legavre, Thierry Ducamp, Michel Sabau, Xavier Argout, Xavier Fouet, Olivier Dedieu, Frédéric Surujdeo-Maharaj, Surendra Garcia, Dominique Paulin, Didier Lanaud, Claire eng 2015 Physiological and Molecular Plant Pathology F30 - Génétique et amélioration des plantes H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Theobroma cacao Phytophthora megasperma résistance génétique expression des gènes relation hôte pathogène inhibiteur d'enzyme protéase PCR hybridation physiologie végétale gène http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_27305 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2599 http://aims.fao.org/aos/agrovoc/c_6242 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3706 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3214 Papouasie-Nouvelle-Guinée Trinité-et-Tobago France http://aims.fao.org/aos/agrovoc/c_5555 http://aims.fao.org/aos/agrovoc/c_7933 http://aims.fao.org/aos/agrovoc/c_3081 Cocoa black pod caused by Phytophthora is one of the most serious diseases affecting cocoa production. This project set out was to improve our knowledge of the molecular mechanisms involved in Theobroma cacao resistance. We performed a transcriptional screening using macroarrays containing 89 ESTs from subtractive libraries enriched with genes differentially expressed in response to T. cacao/Phytophthora interactions. A gene expression analysis revealed the induction of genes involved in stress signal transduction, general and specific defence genes and genes involved in hormone signalling pathways. RT-qPCR showed that the level expression of two genes in the resistant plant was higher during the first eight hours after pathogen inoculation. Three genes from a family encoding a protease inhibitor displayed different levels of expression depending on resistant and susceptible T. cacao clones. A comparison of promoter sequences revealed a motif present only in genes that were up-expressed in the resistant plant. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/577414/1/577414.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.pmpp.2015.08.005 10.1016/j.pmpp.2015.08.005 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.pmpp.2015.08.005 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.pmpp.2015.08.005 info:eu-repo/semantics/reference/purl/http://esttik.cirad.fr/index.html |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Theobroma cacao Phytophthora megasperma résistance génétique expression des gènes relation hôte pathogène inhibiteur d'enzyme protéase PCR hybridation physiologie végétale gène http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_27305 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2599 http://aims.fao.org/aos/agrovoc/c_6242 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3706 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5555 http://aims.fao.org/aos/agrovoc/c_7933 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Theobroma cacao Phytophthora megasperma résistance génétique expression des gènes relation hôte pathogène inhibiteur d'enzyme protéase PCR hybridation physiologie végétale gène http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_27305 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2599 http://aims.fao.org/aos/agrovoc/c_6242 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3706 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5555 http://aims.fao.org/aos/agrovoc/c_7933 http://aims.fao.org/aos/agrovoc/c_3081 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Theobroma cacao Phytophthora megasperma résistance génétique expression des gènes relation hôte pathogène inhibiteur d'enzyme protéase PCR hybridation physiologie végétale gène http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_27305 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2599 http://aims.fao.org/aos/agrovoc/c_6242 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3706 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5555 http://aims.fao.org/aos/agrovoc/c_7933 http://aims.fao.org/aos/agrovoc/c_3081 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Theobroma cacao Phytophthora megasperma résistance génétique expression des gènes relation hôte pathogène inhibiteur d'enzyme protéase PCR hybridation physiologie végétale gène http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_27305 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2599 http://aims.fao.org/aos/agrovoc/c_6242 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3706 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5555 http://aims.fao.org/aos/agrovoc/c_7933 http://aims.fao.org/aos/agrovoc/c_3081 Legavre, Thierry Ducamp, Michel Sabau, Xavier Argout, Xavier Fouet, Olivier Dedieu, Frédéric Surujdeo-Maharaj, Surendra Garcia, Dominique Paulin, Didier Lanaud, Claire Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection |
description |
Cocoa black pod caused by Phytophthora is one of the most serious diseases affecting cocoa production. This project set out was to improve our knowledge of the molecular mechanisms involved in Theobroma cacao resistance. We performed a transcriptional screening using macroarrays containing 89 ESTs from subtractive libraries enriched with genes differentially expressed in response to T. cacao/Phytophthora interactions. A gene expression analysis revealed the induction of genes involved in stress signal transduction, general and specific defence genes and genes involved in hormone signalling pathways. RT-qPCR showed that the level expression of two genes in the resistant plant was higher during the first eight hours after pathogen inoculation. Three genes from a family encoding a protease inhibitor displayed different levels of expression depending on resistant and susceptible T. cacao clones. A comparison of promoter sequences revealed a motif present only in genes that were up-expressed in the resistant plant. |
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article |
topic_facet |
F30 - Génétique et amélioration des plantes H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Theobroma cacao Phytophthora megasperma résistance génétique expression des gènes relation hôte pathogène inhibiteur d'enzyme protéase PCR hybridation physiologie végétale gène http://aims.fao.org/aos/agrovoc/c_7713 http://aims.fao.org/aos/agrovoc/c_27305 http://aims.fao.org/aos/agrovoc/c_35130 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_2599 http://aims.fao.org/aos/agrovoc/c_6242 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3706 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_5555 http://aims.fao.org/aos/agrovoc/c_7933 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Legavre, Thierry Ducamp, Michel Sabau, Xavier Argout, Xavier Fouet, Olivier Dedieu, Frédéric Surujdeo-Maharaj, Surendra Garcia, Dominique Paulin, Didier Lanaud, Claire |
author_facet |
Legavre, Thierry Ducamp, Michel Sabau, Xavier Argout, Xavier Fouet, Olivier Dedieu, Frédéric Surujdeo-Maharaj, Surendra Garcia, Dominique Paulin, Didier Lanaud, Claire |
author_sort |
Legavre, Thierry |
title |
Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection |
title_short |
Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection |
title_full |
Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection |
title_fullStr |
Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection |
title_full_unstemmed |
Identification of Theobroma cacao genes differentially expressed during Phytophthora megakarya infection |
title_sort |
identification of theobroma cacao genes differentially expressed during phytophthora megakarya infection |
url |
http://agritrop.cirad.fr/577414/ http://agritrop.cirad.fr/577414/1/577414.pdf |
work_keys_str_mv |
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