Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica
Sequence variation analysis of promoter and coding regions of DREB1D gene from 16 coffee plants (10 from C. Arabica and 4 from C. canephora), displaying different phenotypes (tolerance vs.susceptibility) regarding to drought, indicated a low level of diversity at protein level but a high level of diversity in the promoters within coffee plants. Furthermore, we found several indications of association between drought tolerance and the genetic variations on DREB1D promoter region, but not with those from the coding region. A comparison of predicted cis-acting elements for all the promoter sequences suggest the DREB1Dis evolving by the rearrangement of cis-regulatory elements and, as result the alteration of expression patterns of this gene might play a fundamental role on drought adaptation in coffee. Three haplotypes of DREB1D loci likely to be associated to contrasting phenotypes regarding to drought, H_16 and H_15 (tolerant), and H_17 (susceptible) are under characterization by homologous transformation in C. arabica.
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dig-cirad-fr-5773572022-04-15T09:41:37Z http://agritrop.cirad.fr/577357/ http://agritrop.cirad.fr/577357/ Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica. Alves Gabriel Sergio Costa, Dechamp Eveline, Freire Luciana Pereira, Pot David, This Dominique, Paiva L.V., Andrade Alan Carvalho, Marraccini Pierre, Etienne Hervé. 2015. In : Proceedings of the 25th International Conference on Coffee Science. ASIC. Paris : ASIC, 42-47. ISBN 978-2-900212-24-0 International Conference on Coffee Science. 25, Armenia, Colombie, 8 Septembre 2014/13 Septembre 2014. Researchers Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica Alves, Gabriel Sergio Costa Dechamp, Eveline Freire, Luciana Pereira Pot, David This, Dominique Paiva, L.V. Andrade, Alan Carvalho Marraccini, Pierre Etienne, Hervé eng 2015 ASIC Proceedings of the 25th International Conference on Coffee Science F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes F60 - Physiologie et biochimie végétale Sequence variation analysis of promoter and coding regions of DREB1D gene from 16 coffee plants (10 from C. Arabica and 4 from C. canephora), displaying different phenotypes (tolerance vs.susceptibility) regarding to drought, indicated a low level of diversity at protein level but a high level of diversity in the promoters within coffee plants. Furthermore, we found several indications of association between drought tolerance and the genetic variations on DREB1D promoter region, but not with those from the coding region. A comparison of predicted cis-acting elements for all the promoter sequences suggest the DREB1Dis evolving by the rearrangement of cis-regulatory elements and, as result the alteration of expression patterns of this gene might play a fundamental role on drought adaptation in coffee. Three haplotypes of DREB1D loci likely to be associated to contrasting phenotypes regarding to drought, H_16 and H_15 (tolerant), and H_17 (susceptible) are under characterization by homologous transformation in C. arabica. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/577357/1/pb251_alves.pdf text cc_by_nd info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by-nd/4.0/ http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=218437 |
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F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes F60 - Physiologie et biochimie végétale |
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F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes F60 - Physiologie et biochimie végétale Alves, Gabriel Sergio Costa Dechamp, Eveline Freire, Luciana Pereira Pot, David This, Dominique Paiva, L.V. Andrade, Alan Carvalho Marraccini, Pierre Etienne, Hervé Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica |
description |
Sequence variation analysis of promoter and coding regions of DREB1D gene from 16 coffee plants (10 from C. Arabica and 4 from C. canephora), displaying different phenotypes (tolerance vs.susceptibility) regarding to drought, indicated a low level of diversity at protein level but a high level of diversity in the promoters within coffee plants. Furthermore, we found several indications of association between drought tolerance and the genetic variations on DREB1D promoter region, but not with those from the coding region. A comparison of predicted cis-acting elements for all the promoter sequences suggest the DREB1Dis evolving by the rearrangement of cis-regulatory elements and, as result the alteration of expression patterns of this gene might play a fundamental role on drought adaptation in coffee. Three haplotypes of DREB1D loci likely to be associated to contrasting phenotypes regarding to drought, H_16 and H_15 (tolerant), and H_17 (susceptible) are under characterization by homologous transformation in C. arabica. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes H50 - Troubles divers des plantes F60 - Physiologie et biochimie végétale |
author |
Alves, Gabriel Sergio Costa Dechamp, Eveline Freire, Luciana Pereira Pot, David This, Dominique Paiva, L.V. Andrade, Alan Carvalho Marraccini, Pierre Etienne, Hervé |
author_facet |
Alves, Gabriel Sergio Costa Dechamp, Eveline Freire, Luciana Pereira Pot, David This, Dominique Paiva, L.V. Andrade, Alan Carvalho Marraccini, Pierre Etienne, Hervé |
author_sort |
Alves, Gabriel Sergio Costa |
title |
Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica |
title_short |
Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica |
title_full |
Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica |
title_fullStr |
Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica |
title_full_unstemmed |
Characterization of CcDREB1D promoter region from contrasting genotypes of Coffea canephora by homologous transformation in Coffea arabica |
title_sort |
characterization of ccdreb1d promoter region from contrasting genotypes of coffea canephora by homologous transformation in coffea arabica |
publisher |
ASIC |
url |
http://agritrop.cirad.fr/577357/ http://agritrop.cirad.fr/577357/1/pb251_alves.pdf |
work_keys_str_mv |
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