The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants
In plants, host response to pathogenic microbes is driven both by microbial perception and detection of modified-self. The Xanthomonas campestris effector protein AvrAC/XopAC uridylylates the Arabidopsis BIK1 kinase to dampen basal resistance and thereby promotes bacterial virulence. Here we show that PBL2, a paralog of BIK1, is similarly uridylylated by AvrAC. However, in contrast to BIK1, PBL2 uridylylation is specifically required for host recognition of AvrAC to trigger immunity, but not AvrAC virulence. PBL2 thus acts as a decoy and enables AvrAC detection. AvrAC recognition also requires the RKS1 pseudokinase of the ZRK family and the NOD-like receptor ZAR1, which is known to recognize the Pseudomonas syringae effector HopZ1a. ZAR1 forms a stable complex with RKS1, which specifically recruits PBL2 when the latter is uridylylated by AvrAC, triggering ZAR1-mediated immunity. The results illustrate how decoy substrates and pseudokinases can specify and expand the capacity of the plant immune system.
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dig-cirad-fr-5773112024-01-28T22:51:38Z http://agritrop.cirad.fr/577311/ http://agritrop.cirad.fr/577311/ The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants. Wang Guoxun, Roux Brice, Feng Feng, Guy Endrick, Li Lin, Li Nannan, Zhang Xiaojuan, Lautier Martine, Jardinaud Marie-Françoise, Chabannes Matthieu, Arlat Mathieu, Chen She, He Chaozu, Noel Laurent D., Zhou Jian-Min. 2015. Cell Host and Microbe, 18 (3) : 285-295.https://doi.org/10.1016/j.chom.2015.08.004 <https://doi.org/10.1016/j.chom.2015.08.004> The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants Wang, Guoxun Roux, Brice Feng, Feng Guy, Endrick Li, Lin Li, Nannan Zhang, Xiaojuan Lautier, Martine Jardinaud, Marie-Françoise Chabannes, Matthieu Arlat, Mathieu Chen, She He, Chaozu Noel, Laurent D. Zhou, Jian-Min eng 2015 Cell Host and Microbe H20 - Maladies des plantes Xanthomonas campestris mécanisme de défense cellulaire effecteur moléculaire immunologie pouvoir pathogène résistance aux maladies Arabidopsis génie génétique génétique moléculaire Pseudomonas syringae plante de culture plante hôte kinase http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_11621 http://aims.fao.org/aos/agrovoc/c_11977 France Chine http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1556 In plants, host response to pathogenic microbes is driven both by microbial perception and detection of modified-self. The Xanthomonas campestris effector protein AvrAC/XopAC uridylylates the Arabidopsis BIK1 kinase to dampen basal resistance and thereby promotes bacterial virulence. Here we show that PBL2, a paralog of BIK1, is similarly uridylylated by AvrAC. However, in contrast to BIK1, PBL2 uridylylation is specifically required for host recognition of AvrAC to trigger immunity, but not AvrAC virulence. PBL2 thus acts as a decoy and enables AvrAC detection. AvrAC recognition also requires the RKS1 pseudokinase of the ZRK family and the NOD-like receptor ZAR1, which is known to recognize the Pseudomonas syringae effector HopZ1a. ZAR1 forms a stable complex with RKS1, which specifically recruits PBL2 when the latter is uridylylated by AvrAC, triggering ZAR1-mediated immunity. The results illustrate how decoy substrates and pseudokinases can specify and expand the capacity of the plant immune system. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/577311/1/Wang%20et%20al.%2C%202015.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.chom.2015.08.004 10.1016/j.chom.2015.08.004 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chom.2015.08.004 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.chom.2015.08.004 |
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H20 - Maladies des plantes Xanthomonas campestris mécanisme de défense cellulaire effecteur moléculaire immunologie pouvoir pathogène résistance aux maladies Arabidopsis génie génétique génétique moléculaire Pseudomonas syringae plante de culture plante hôte kinase http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_11621 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1556 H20 - Maladies des plantes Xanthomonas campestris mécanisme de défense cellulaire effecteur moléculaire immunologie pouvoir pathogène résistance aux maladies Arabidopsis génie génétique génétique moléculaire Pseudomonas syringae plante de culture plante hôte kinase http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_11621 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1556 |
spellingShingle |
H20 - Maladies des plantes Xanthomonas campestris mécanisme de défense cellulaire effecteur moléculaire immunologie pouvoir pathogène résistance aux maladies Arabidopsis génie génétique génétique moléculaire Pseudomonas syringae plante de culture plante hôte kinase http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_11621 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1556 H20 - Maladies des plantes Xanthomonas campestris mécanisme de défense cellulaire effecteur moléculaire immunologie pouvoir pathogène résistance aux maladies Arabidopsis génie génétique génétique moléculaire Pseudomonas syringae plante de culture plante hôte kinase http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_11621 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1556 Wang, Guoxun Roux, Brice Feng, Feng Guy, Endrick Li, Lin Li, Nannan Zhang, Xiaojuan Lautier, Martine Jardinaud, Marie-Françoise Chabannes, Matthieu Arlat, Mathieu Chen, She He, Chaozu Noel, Laurent D. Zhou, Jian-Min The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants |
description |
In plants, host response to pathogenic microbes is driven both by microbial perception and detection of modified-self. The Xanthomonas campestris effector protein AvrAC/XopAC uridylylates the Arabidopsis BIK1 kinase to dampen basal resistance and thereby promotes bacterial virulence. Here we show that PBL2, a paralog of BIK1, is similarly uridylylated by AvrAC. However, in contrast to BIK1, PBL2 uridylylation is specifically required for host recognition of AvrAC to trigger immunity, but not AvrAC virulence. PBL2 thus acts as a decoy and enables AvrAC detection. AvrAC recognition also requires the RKS1 pseudokinase of the ZRK family and the NOD-like receptor ZAR1, which is known to recognize the Pseudomonas syringae effector HopZ1a. ZAR1 forms a stable complex with RKS1, which specifically recruits PBL2 when the latter is uridylylated by AvrAC, triggering ZAR1-mediated immunity. The results illustrate how decoy substrates and pseudokinases can specify and expand the capacity of the plant immune system. |
format |
article |
topic_facet |
H20 - Maladies des plantes Xanthomonas campestris mécanisme de défense cellulaire effecteur moléculaire immunologie pouvoir pathogène résistance aux maladies Arabidopsis génie génétique génétique moléculaire Pseudomonas syringae plante de culture plante hôte kinase http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_37523 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_1972 http://aims.fao.org/aos/agrovoc/c_11621 http://aims.fao.org/aos/agrovoc/c_11977 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1556 |
author |
Wang, Guoxun Roux, Brice Feng, Feng Guy, Endrick Li, Lin Li, Nannan Zhang, Xiaojuan Lautier, Martine Jardinaud, Marie-Françoise Chabannes, Matthieu Arlat, Mathieu Chen, She He, Chaozu Noel, Laurent D. Zhou, Jian-Min |
author_facet |
Wang, Guoxun Roux, Brice Feng, Feng Guy, Endrick Li, Lin Li, Nannan Zhang, Xiaojuan Lautier, Martine Jardinaud, Marie-Françoise Chabannes, Matthieu Arlat, Mathieu Chen, She He, Chaozu Noel, Laurent D. Zhou, Jian-Min |
author_sort |
Wang, Guoxun |
title |
The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants |
title_short |
The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants |
title_full |
The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants |
title_fullStr |
The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants |
title_full_unstemmed |
The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants |
title_sort |
decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants |
url |
http://agritrop.cirad.fr/577311/ http://agritrop.cirad.fr/577311/1/Wang%20et%20al.%2C%202015.pdf |
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