Molecular epidemiology of cassava mosaic disease in Madagascar
Cassava is the staple food for hundreds of millions of people in Africa but its cultivation is seriously constrained by cassava mosaic disease (CMD) in Madagascar, and in Africa in general. This study identified the cassava mosaic geminiviruses (CMGs) involved in CMD in Madagascar and their associated epidemiological characteristics from countrywide surveys. Molecular characterization of CMGs in Madagascar revealed an unprecedented diversity and co-occurrence of six viruses: African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Kenya virus (EACMKV), East African cassava mosaic virus (EACMV), South African cassava mosaic virus (SACMV) and the recently described Cassava mosaic Madagascar virus (CMMGV). Distinct geographical distributions were observed for the six viruses. While ACMV was more prevalent in the central highlands, EACMV and EACMKV were prevalent in lowlands and coastal regions. Both EACMCV and SACMV occurred in almost all the localities visited. PCR diagnosis revealed that mixed infection (up to four co-infected viruses) occurred in 21% of the samples and were associated with higher symptom severity scores. Pairwise comparisons of virus associations showed that EACMCV was found in mixed infections more often than expected while ACMV and SACMV were mostly found in single infections. A greater abundance of whiteflies was observed in lowland and coastal areas. Nevertheless, infected cuttings remain the primary source of CMD propagation (95%) in Madagascar.
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Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, virus des végétaux, Manihot esculenta, Geminiviridae, épidémiologie, diagnostic, biologie moléculaire, PCR, distribution géographique, virus mosaïque manioc, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_4579, http://aims.fao.org/aos/agrovoc/c_32859, http://aims.fao.org/aos/agrovoc/c_2615, http://aims.fao.org/aos/agrovoc/c_2238, http://aims.fao.org/aos/agrovoc/c_4891, http://aims.fao.org/aos/agrovoc/c_34079, http://aims.fao.org/aos/agrovoc/c_5083, http://aims.fao.org/aos/agrovoc/c_33962, http://aims.fao.org/aos/agrovoc/c_4510, |
Online Access: | http://agritrop.cirad.fr/576323/ http://agritrop.cirad.fr/576323/1/576323.pdf |
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dig-cirad-fr-5763232024-01-28T22:45:00Z http://agritrop.cirad.fr/576323/ http://agritrop.cirad.fr/576323/ Molecular epidemiology of cassava mosaic disease in Madagascar. Harimalala Mireille Aurélie, Chiroleu Frédéric, Giraud-Carrier Charlotte, Hoareau Marie Murielle, Zinga Innocent, Randriamampianina Jean Augustin, Velombola S., Ranomenjanahary Sahondramalala, Andrianjaka Alice, Reynaud Bernard, Lefeuvre Pierre, Lett Jean-Michel. 2015. Plant Pathology, 64 (3) : 501-507.https://doi.org/10.1111/ppa.12277 <https://doi.org/10.1111/ppa.12277> Molecular epidemiology of cassava mosaic disease in Madagascar Harimalala, Mireille Aurélie Chiroleu, Frédéric Giraud-Carrier, Charlotte Hoareau, Marie Murielle Zinga, Innocent Randriamampianina, Jean Augustin Velombola, S. Ranomenjanahary, Sahondramalala Andrianjaka, Alice Reynaud, Bernard Lefeuvre, Pierre Lett, Jean-Michel eng 2015 Plant Pathology H20 - Maladies des plantes U30 - Méthodes de recherche virus des végétaux Manihot esculenta Geminiviridae épidémiologie diagnostic biologie moléculaire PCR distribution géographique virus mosaïque manioc http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_4579 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_33962 Madagascar http://aims.fao.org/aos/agrovoc/c_4510 Cassava is the staple food for hundreds of millions of people in Africa but its cultivation is seriously constrained by cassava mosaic disease (CMD) in Madagascar, and in Africa in general. This study identified the cassava mosaic geminiviruses (CMGs) involved in CMD in Madagascar and their associated epidemiological characteristics from countrywide surveys. Molecular characterization of CMGs in Madagascar revealed an unprecedented diversity and co-occurrence of six viruses: African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Kenya virus (EACMKV), East African cassava mosaic virus (EACMV), South African cassava mosaic virus (SACMV) and the recently described Cassava mosaic Madagascar virus (CMMGV). Distinct geographical distributions were observed for the six viruses. While ACMV was more prevalent in the central highlands, EACMV and EACMKV were prevalent in lowlands and coastal regions. Both EACMCV and SACMV occurred in almost all the localities visited. PCR diagnosis revealed that mixed infection (up to four co-infected viruses) occurred in 21% of the samples and were associated with higher symptom severity scores. Pairwise comparisons of virus associations showed that EACMCV was found in mixed infections more often than expected while ACMV and SACMV were mostly found in single infections. A greater abundance of whiteflies was observed in lowland and coastal areas. Nevertheless, infected cuttings remain the primary source of CMD propagation (95%) in Madagascar. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/576323/1/576323.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/ppa.12277 10.1111/ppa.12277 info:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.12277 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/ppa.12277 |
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H20 - Maladies des plantes U30 - Méthodes de recherche virus des végétaux Manihot esculenta Geminiviridae épidémiologie diagnostic biologie moléculaire PCR distribution géographique virus mosaïque manioc http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_4579 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_33962 http://aims.fao.org/aos/agrovoc/c_4510 H20 - Maladies des plantes U30 - Méthodes de recherche virus des végétaux Manihot esculenta Geminiviridae épidémiologie diagnostic biologie moléculaire PCR distribution géographique virus mosaïque manioc http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_4579 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_33962 http://aims.fao.org/aos/agrovoc/c_4510 |
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H20 - Maladies des plantes U30 - Méthodes de recherche virus des végétaux Manihot esculenta Geminiviridae épidémiologie diagnostic biologie moléculaire PCR distribution géographique virus mosaïque manioc http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_4579 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_33962 http://aims.fao.org/aos/agrovoc/c_4510 H20 - Maladies des plantes U30 - Méthodes de recherche virus des végétaux Manihot esculenta Geminiviridae épidémiologie diagnostic biologie moléculaire PCR distribution géographique virus mosaïque manioc http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_4579 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_33962 http://aims.fao.org/aos/agrovoc/c_4510 Harimalala, Mireille Aurélie Chiroleu, Frédéric Giraud-Carrier, Charlotte Hoareau, Marie Murielle Zinga, Innocent Randriamampianina, Jean Augustin Velombola, S. Ranomenjanahary, Sahondramalala Andrianjaka, Alice Reynaud, Bernard Lefeuvre, Pierre Lett, Jean-Michel Molecular epidemiology of cassava mosaic disease in Madagascar |
description |
Cassava is the staple food for hundreds of millions of people in Africa but its cultivation is seriously constrained by cassava mosaic disease (CMD) in Madagascar, and in Africa in general. This study identified the cassava mosaic geminiviruses (CMGs) involved in CMD in Madagascar and their associated epidemiological characteristics from countrywide surveys. Molecular characterization of CMGs in Madagascar revealed an unprecedented diversity and co-occurrence of six viruses: African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Kenya virus (EACMKV), East African cassava mosaic virus (EACMV), South African cassava mosaic virus (SACMV) and the recently described Cassava mosaic Madagascar virus (CMMGV). Distinct geographical distributions were observed for the six viruses. While ACMV was more prevalent in the central highlands, EACMV and EACMKV were prevalent in lowlands and coastal regions. Both EACMCV and SACMV occurred in almost all the localities visited. PCR diagnosis revealed that mixed infection (up to four co-infected viruses) occurred in 21% of the samples and were associated with higher symptom severity scores. Pairwise comparisons of virus associations showed that EACMCV was found in mixed infections more often than expected while ACMV and SACMV were mostly found in single infections. A greater abundance of whiteflies was observed in lowland and coastal areas. Nevertheless, infected cuttings remain the primary source of CMD propagation (95%) in Madagascar. |
format |
article |
topic_facet |
H20 - Maladies des plantes U30 - Méthodes de recherche virus des végétaux Manihot esculenta Geminiviridae épidémiologie diagnostic biologie moléculaire PCR distribution géographique virus mosaïque manioc http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_4579 http://aims.fao.org/aos/agrovoc/c_32859 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_2238 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_5083 http://aims.fao.org/aos/agrovoc/c_33962 http://aims.fao.org/aos/agrovoc/c_4510 |
author |
Harimalala, Mireille Aurélie Chiroleu, Frédéric Giraud-Carrier, Charlotte Hoareau, Marie Murielle Zinga, Innocent Randriamampianina, Jean Augustin Velombola, S. Ranomenjanahary, Sahondramalala Andrianjaka, Alice Reynaud, Bernard Lefeuvre, Pierre Lett, Jean-Michel |
author_facet |
Harimalala, Mireille Aurélie Chiroleu, Frédéric Giraud-Carrier, Charlotte Hoareau, Marie Murielle Zinga, Innocent Randriamampianina, Jean Augustin Velombola, S. Ranomenjanahary, Sahondramalala Andrianjaka, Alice Reynaud, Bernard Lefeuvre, Pierre Lett, Jean-Michel |
author_sort |
Harimalala, Mireille Aurélie |
title |
Molecular epidemiology of cassava mosaic disease in Madagascar |
title_short |
Molecular epidemiology of cassava mosaic disease in Madagascar |
title_full |
Molecular epidemiology of cassava mosaic disease in Madagascar |
title_fullStr |
Molecular epidemiology of cassava mosaic disease in Madagascar |
title_full_unstemmed |
Molecular epidemiology of cassava mosaic disease in Madagascar |
title_sort |
molecular epidemiology of cassava mosaic disease in madagascar |
url |
http://agritrop.cirad.fr/576323/ http://agritrop.cirad.fr/576323/1/576323.pdf |
work_keys_str_mv |
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